Stent-induced neutrophil activation is associated with an oxidative burst in the inflammatory process, leading to neointimal thickening

Teruo Inoue; Toru Kato; Yutaka Hikichi; Shigemasa Hashimoto; Tetsuaki Hirase; Toshifumi Morooka; Yoshitaka Imoto; Yuji Takeda; Fujiro Sendo; Koichi Node

doi:10.1160/TH05-08-0591

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2006; 95(01): 43-48
DOI: 10.1160/TH05-08-0591

Theme Issue Article

Schattauer GmbH

Stent-induced neutrophil activation is associated with an oxidative burst in the inflammatory process, leading to neointimal thickening

Authors

Teruo Inoue

¹Department of Cardiovascular and Renal Medicine, Saga University Faculty of Medicine, Saga, Japan
Toru Kato

¹Department of Cardiovascular and Renal Medicine, Saga University Faculty of Medicine, Saga, Japan
Yutaka Hikichi

¹Department of Cardiovascular and Renal Medicine, Saga University Faculty of Medicine, Saga, Japan
Shigemasa Hashimoto

¹Department of Cardiovascular and Renal Medicine, Saga University Faculty of Medicine, Saga, Japan
Tetsuaki Hirase

¹Department of Cardiovascular and Renal Medicine, Saga University Faculty of Medicine, Saga, Japan
Toshifumi Morooka

¹Department of Cardiovascular and Renal Medicine, Saga University Faculty of Medicine, Saga, Japan
Yoshitaka Imoto

²Yufu Itonaga Co., Ltd., Tokyo Japan
Yuji Takeda

³Japan Immunoresearch Institute, Takasaki, Japan
Fujiro Sendo

⁴Yamagata University, Yamagata, Japan
Koichi Node

¹Department of Cardiovascular and Renal Medicine, Saga University Faculty of Medicine, Saga, Japan

Abstract

Summary

Activation of leukocytes plays an essential role in the mechanism of restenosis. Prior research has focused on monocytes and little is known about the role of neutrophils in this process. Neutrophils are known to contribute to tissue injury through oxygen-derived free radicals that nitrate tyrosine. This study was designed to elucidate clinically the role of neutrophil-mediated oxidative burst in the regulation of the post-stent inflammatory process. In 36 patients undergoing coronary stenting, we serially measured serum levels of glycosyl-phosphatidil-inositol-anchored protein (GPI)-80, a modulator of Mac-1 on the surface of neutrophils, in samples of coronary sinus as well as peripheral blood. We also simultaneously measured the serum 3-nitrotyrosine/tyrosine ratio as an index of oxidative stress. The GPI-80 level and the 3-nitrotyrosine/tyrosine ratio increased in the coronary sinus after coronary stenting in a time-dependent manner; with the maximum increase of GPI-80 level (3.1±2.9 to 8.6±4.3 ng/ml, P<0.01) at 48 hours, and 3-nitrotyrosine/tyrosine ratio at 24 hrs (5.2±4.8 to 28.4±13.2 ×10⁻⁴, P<0.01), more strikingly than in the peripheral blood. In the coronary sinus blood, the 3-nitrotyrosine/tyrosine ratio was correlated with GPI-80 levels at 24 hr (R=0.58, P<0. 001) and at 48 hr (R=0.41, P<0.01). Multiple regressions analysis showed that the maximum responses of GPI-80 level and 3-nitrotyrosine/tyrosine ratio were independent predictors of angiographic late lumen loss. Our results may supporta hypothesis that Mac-1-dependent activation of neutrophils causes oxidative burst in the post-stent inflammatory process, possibly leading to restenosis.

Keywords

Neutrophil - Mac-1 - GPI-80 - nitrotyrosine - restenosis

Full Text

References

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