Plasma factor Xa inhibition can predict antithrombotic effects of oral direct factor Xa inhibitors in rabbit atherothrombosis models

Toshiyuki Funatsu; Atsushi Yamashita; Seiji Kaku; Yoshiyuki Iwatsuki; Yujiro Asada

doi:10.1160/TH12-04-0267

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 2012; 108(05): 896-902
DOI: 10.1160/TH12-04-0267

Blood Coagulation, Fibrinolysis and Cellular Haemostasis

Schattauer GmbH

Plasma factor Xa inhibition can predict antithrombotic effects of oral direct factor Xa inhibitors in rabbit atherothrombosis models

Toshiyuki Funatsu

¹Applied Pharmacology Research Laboratories, Ibaraki, Japan

,

Atsushi Yamashita

²Department of Pathology, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan

,

Seiji Kaku

¹Applied Pharmacology Research Laboratories, Ibaraki, Japan

,

Yoshiyuki Iwatsuki

¹Applied Pharmacology Research Laboratories, Ibaraki, Japan

,

Yujiro Asada

²Department of Pathology, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan

› Institutsangaben

Abstract

Summary

We evaluated the relationship between antithrombotic effects and pharmacodynamic (PD) marker changes produced by the novel factor (F)Xa inhibitors darexaban (YM150) and rivaroxaban in a rabbit model of plaque disruption-induced arterial thrombosis. Animals were subjected to catheter-induced endothelial denudation via the femoral artery followed by a two-week high-cholesterol diet. Plaque disruption was induced by balloon angioplasty, and then stasis was achieved by ligation at the distal side of the injured segment. Darexaban and rivaroxaban were administered orally 1 hour (h) before and 9 h after plaque disruption, and their antithrombotic effects were evaluated 24 h after the initiation of ligation. Prothrombin time (PT), activated partial thromboplastin time (APTT), and plasma FXa activity were measured using blood samples collected before and 1 h after administration. Darexaban and rivaroxaban significantly reduced thrombus formation. The thrombus weight obtained in the 30 mg/kg darexaban group was comparable to that in the 1 mg/kg rivaroxaban group (2.17 ± 0.63 and 3.23 ± 1.64 mg, respectively, vs. 8.01 ± 1.08 mg in the control group). Plasma FXa activity correlated with the antithrombotic effects of darexaban and rivaroxaban, while PT only correlated with those of darexaban. Our findings suggest that the degree of plasma FXa inhibition may be useful for predicting antithrombotic effects of darexaban and rivaroxaban in arterial thrombosis. PT may also be useful in evaluating antithrombotic effects of darexaban in particular.

Keywords

Animal model - darexaban - FXa assay - rivaroxaban - thrombosis

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