Tierarztl Prax Ausg G Grosstiere Nutztiere 2016; 44(04): 259-265
DOI: 10.15653/TPG-160100
Kasuistik
Schattauer GmbH

Influenza-Ausbruch bei Aufzuchtferkeln unter Beteiligung von Mycoplasma hyorhinis und Haemophilus parasuis

Ein FallberichtInfluenza outbreak in weaners with involvement of Mycoplasma hyorhinis and Haemophilus parasuis A case report
Christine Unterweger
1   Universitätsklinik für Schweine, Veterinärmedizinische Universität Wien, Österreich
,
Bettina Wöchtl
1   Universitätsklinik für Schweine, Veterinärmedizinische Universität Wien, Österreich
,
Joachim Spergser
2   Institut für Mikrobiologie, Veterinärmedizinische Universität Wien, Österreich
,
Rene Brunthaler
3   Institut für Pathologie, Veterinärmedizinische Universität Wien, Österreich
,
Matthias Untersperger
4   Tierarztpraxis Untersperger, Bad Hall, Österreich
,
Kathrin Lillie-Jaschniski
5   IDT Biologika GmbH, Dessau-Rosslau, Deutschland
,
Ralf Dürrwald
5   IDT Biologika GmbH, Dessau-Rosslau, Deutschland
,
Isabel Hennig-Pauka
1   Universitätsklinik für Schweine, Veterinärmedizinische Universität Wien, Österreich
› Author Affiliations
Further Information

Publication History

Received: 20 January 2016

Accepted after major revision: 13 April 2016

Publication Date:
23 December 2017 (online)

Zusammenfassung

In einem geschlossenen Ferkelerzeugerbetrieb erkrankten etwa 80% der 7 Wochen alten Ferkel in der Aufzucht an einer fieberhaften Atemwegsinfektion mit einer Letalität von 5%. Neben eitrigen Broncho - pneumonien in Kombination mit interstitiellen Pneumonien insbesondere im Spitzen- und Mittellappenbereich lagen fibrinöse Serositiden in der Brust- und Bauchhöhle vor. Weiterführend gelang der Nachweis des nichtpandemischen Stamms von porzinem Influenza-A-Virus (FLUAVsw) Subtyp H1avN1. Die molekulargenetischen Untersuchungen auf Mycoplasma (M.) hyopneumoniae und PRRSV verliefen negativ, doch konnten M. hyorhinis und Haemophilus parasuis aus serösen Häuten nach - gewiesen werden. Die möglicherweise unterschätzte Bedeutung von M. hyorhinis als Kofaktor bei Virusinfektionen und Verursacher von Spitzenlappenpneumonien wird hervorgehoben und aufgezeigt. Die Muttersauen des Bestands waren mit einem Influenzaimpfstoff geimpft worden, der den Subtyp H1avN1 abdeckt. Nur 33% der untersuchten Ferkel hatten in der 7. Lebenswoche noch maternale Anti körper. Die Schwierigkeit einer Prophylaxe von Erkrankungen durch FLUAVsw bei Ferkeln mit nachlassender maternaler Immunität wird deutlich, wenn Impfstoffe erst ab dem 56. Lebenstag eingesetzt werden dürfen.

Summary

In a closed farrow-to-finish piglet producing farm 80% of 7-week-old piglets displayed respiratory disease with a 5% mortality rate. In addition to purulent bronchopneumonia in combination with interstitial pneumonia predominantly in the apical and middle lobes, fibrinous sero sitis was present in the thoracic and abdominal cavities. Further in vestigations succeeded in confirming the non-pandemic strain of porcine influenza A virus (FLUAVsw) subtype H1avN1. The molecular genetic studies on Mycoplasma (M.) hyopneumoniae and porcine reproductive and respiratory syndrome virus were negative, whereas M. hyorhinis and Haemophilus parasuis were isolated from serous membranes. The possible importance of the underrated M. hyorhinis as a cofactor for viral infections should be emphasized and we de - monstrated that the cause of apical lobe pneumonia is not restricted to M. hyopneumoniae. Mother pigs had been vaccinated with an influenza vaccine covering the subtype H1avN1. Only 33% of the exa - mined piglets had maternal antibodies in the 7th week of life. The difficulty of prophylaxis of infections by FLUAVsw in weaners due to lack of vaccine authorization for piglets before their 56th day is reflected by this observation.

 
  • Literatur

  • 1 Allerson MW, Cardona CJ, Torremorell M. Indirect transmission of Influenza A Virus between pig populations under two different biosecurity settings. PloS One 2013; 8: e67293.
  • 2 Corn JL, Cumbee JC, Barfoot R, Erickson GA. Pathogen exposure in feral swine populations geographically associated with high densities of transitional swine premises and commercial swine production. J Wildl Dis 2009; 45: 713-721.
  • 3 Corzo CA, Allerson M, Gramer M, Morrison RB, Torremorell M. Detection of airborne influenza A virus in experimentally infected pigs with maternally derived antibodies. Transbound Emerg Dis 2014; 61: 28-36.
  • 4 Corzo CA, Gramer MR, Kuhn M, Mohr M, Morrison R. Observations regarding influenza A virus shedding in a swine breeding farm after mass vaccination. J Swine Health Prod 2012; 20: 283-289.
  • 5 Gois M, Kuksa F. Intranasal infection of gnotobiotic piglets with M. hyorhinis: differences in virulence of the strains and influence of age on the development of infection. Zbl Vet Med B 1974; 21: 352-361.
  • 6 Hansen MS, Pors SE, Jensen HE, Bille-Hansen V, Bisgaard M, Flachs EM, Nielsen OL. An investigation of the pathology and pathogens associated with porcine respiratory disease complex in Denmark. J Comp Path 2010; 143: 120-131.
  • 7 Heinen PP, van Nieuwstadt AP, Pol JM, de Boer-Luijtze EA, van Oirschot JT, Bianchi AT. Systemic and mucosal isotype-specific antibody responses in pigs to experimental influenza virus infection. Viral Immunol 2000; 13: 237-247.
  • 8 Kang I, Kim D, Han K, Seo HW, Oh Y, Park C, Lee I, Gottschalk M, Chae C. Optimized protocol for multiplex nested polymerase chain reaction to detect and differentiate Haemophilus parasuis, Streptococcus suis, and Mycoplasma hyorhinis in formalin-fixed, paraffin-embedded tissues from pigs with polyserositis. Can J Vet Res 2012; 76: 195-200.
  • 9 Kinne J, Johannsen U, Neumann R, Mehlhorn G, Pfützner H. The pathology and pathogenesis of experimental Mycoplasma hyorhinis infection of piglets with and without thermomotoric stress. 1. Pathologico-anatomic, histologic and immunomorphologic study results. Zentralbl Veterinarmed A 1991; 38: 306-320.
  • 10 Kitikoon P, Nilubo D, Erickson BJ, Janke BH, Hoover TC, Sornsen SA, Thacker EL. The immune response and maternal antibody interference to a heterologous H1N1 swine influenza virus infection following vaccination. Vet Immunol Immunopathol 2006; 112: 117-128.
  • 11 Kitikoon P, Gauger PC, Vincent AL. Hemagglutinin inhibition assay with swine sera. Animal Influenza Virus. Methods in Molecular Biology.. Spackmann E. New York (USA): Springer; 2014. 1161 295-301.
  • 12 Kobisch M. Pathogenicity of Mycoplasma hyorhinis . Yale J Biol Med 1983; 56: 922-923.
  • 13 Kurth KT, Hsu T, Snook ER, Thacker EL, Thacker BJ, Minion FC. Use of a Mycoplasma hyopneumoniae nested polymerase chain reaction test to determine the optimal sampling sites in swine. J Vet Diagn Invest 2002; 14: 463-469.
  • 14 Kyriakis CS, Olsen CW, Carman S, Brown IH, Brookes SM, Doorsselaere JV, Van Reeth K. Serologic cross-reactivity with pandemic (H1N1) 2009 virus in pigs, Europe. Emerg Infect Dis 2010; 16: 96-99.
  • 15 Kyriakis CS, Brown IH, Foni E, Kuntz-Simon G, Maldonado J, Madec F, Essen SC, Chiapponi C, Van Reeth K. Virological surveillance and preliminary antigenic characterization of influenza viruses in pigs in five European countries from 2006 to 2008. Zoonoses Public Health 2011; 58: 93-101.
  • 16 Lange J, Groth M, Schlegel M, Krumbholz A, Wieczorek K, Ulrich R, Köppen S, Schulz K, Appl D, Selbitz HJ, Sauerbrei A, Platzer M, Zell R, Dürrwald R. Reassortants of the pandemic (H1N1) 2009 virus and establishment of a novel porcine H1N2 influenza virus, lineage in Germany. Vet Microbiol 2013; 167: 345-356.
  • 17 Lin JH, Chen SP, Yeh KS, Weng CN. Mycoplasma hyorhinis in Taiwan: diagnosis and isolation of swine pneumonia pathogen. Vet Microbiol 2006; 115: 111-116.
  • 18 Loeffen WL, Heinen PP, Bianchi AT, Hunneman WA, Verheijden JH. Effect of maternally derived antibodies on the clinical signs and immune response in pigs after primary and secondary infection with an influenza H1N1 virus. Vet Immunol Immunopathol 2003; 92: 23-35.
  • 19 Loeffen WL, Hunneman WA, Quak J, Verheijden HJ, Stegeman JA. Population dynamics of swine influenza virus in farrow-to-finish and specialised finishing herds in the Netherlands. Vet Microbiol 2009; 137: 45-50.
  • 20 Ma W, Kahn RE, Richt JA. The pig as a mixing vessel for influenza viruses: Human and veterinary implications. J Mol Genet Med 2009; 3: 158-166.
  • 21 Markowska-Daniel I, Pomorska-Mol M, Pejsak Z. The influence of age and maternal antibodies on the postvaccinal response against swine influenza viruses in pigs. Vet Immunol Immunopathol 2011; 142: 81-86.
  • 22 Meiners C, Loesken S, Doehring S, Starick E, Pesch S, Maas A, Noe T, Beer M, Harder T, Grosse Beilage E. Field study on swine influenza virus (SIV) infection in weaner pigs and sows. Tierarztl Prax 2014; 42(G): 351-359.
  • 23 Olsen CW, Karasin AI, Carman S, Li Y, Bastien N, Ojkic D, Alves D, Charbonneau G, Henning BM, Low DE, Burton L, Broukhanski G. Triple reassortant H3N2 influenza A viruses, Canada, 2005. Emerg Infect Dis 2006; 12: 1132-1135.
  • 24 Palzer A, Ritzmann M, Wolf G, Heinritzi K. Associations between pathogens in healthy pigs and pigs with pneumonia. Vet Rec 2008; 162: 267-271.
  • 25 Palzer A, Haedke K, Heinritzi K, Zoels S, Ladinig A, Ritzmann M. Associations among Haemophilus parasuis, Mycoplasma hyorhinis, and porcine reproductive and respiratory syndrome virus infections in pigs with polyserositis. Can Vet J 2015; 56: 285-287.
  • 26 Palzer A, Kolb K, Strutzberg-Minder K, Zoels S, Eddicks M, Heinritzi K, Ritzmann M. Serological course investigations of Haemophilus parasuis and Mycoplasma hyorhinis in three pig farms. Schweiz Arch Tierheilkd 2015; 157: 97-103.
  • 27 Romagosa A, Allerson M, Gramer M, Joo HS, Deen J, Detmer S, Torremorell M. Vaccination of influenza A virus decreases transmission rates in pigs. Vet Res 2011; 42: 120-135.
  • 28 Sandbulte MR, Spickler AR, Zaabel PK, Roth JA. Optimal use of vaccines for control of influenza A Virus in swine. Vaccines 2015; 3: 22-73.
  • 29 Simon G, Larsen LE, Dürrwald R, Foni E, Harder T, Van Reeth K, Markowska-Daniel I, Reid SM, Dan A, Maldonado J, Huovilainen A, Billinis C, Davidson I, Aguero M, Vila T, Herve S, Breum SO, Chiappon C, Urbaniak K, Kyriakis CS, Brown IH, Loeffen W. European surveillance network for influenza in pigs: surveillance programs, diagnostic tools and Swine influenza virus subtypes identified in 14 European countries from 2010 to 2013. PloS One 2014; 9: e115815.
  • 30 Strain EL, Madsen ML, Minion FC, Christopher-Hennings J, Dammen M, Jones KR, Thacker EL, Real-Time PCR. assays to address genetic diversity among strains of Mycoplasma hyopneumoniae . J Clin Microbiol 2008; 46: 2491-2498.
  • 31 Thacker EL, Thacker BJ, Janke BH. Interaction between Mycoplasma hyopneumoniae and swine influenza virus. J Clin Microbiol 2001; 39: 2525-2530.
  • 32 Thacker EL, Minion FC. Mycoplasmosis. Diseases of Swine. 10th Zimmerman JJ, Karriker LA, Ramirez A, Schwartz KJ, Stevenson GW. Ames (Iowa, USA): Wiley-Blackwell; 779-797.
  • 33 Tse M, Kim M, Chan CH, Ho PL, Ma SK, Guan Y, Peiris JSM. Evaluation of three commercially available influenza A type-specific blocking enzymelinked immunosorbent assays for seroepidemiological studies of influenza A virus infection in pigs. Clin Vaccine Immunol 2012; 19: 334-337.
  • 34 Van Reeth K, Nauwynck H, Pensaert M. Dual infections of feeder pigs with porcine reproductive and respiratory syndrome virus followed by porcine respiratory coronavirus or swine influenza virus: a clinical and virological study. Vet Microbiol 1996; 48: 325-335.
  • 35 Van Reeth K, Brown IH, Dürrwald R, Foni E, Labarque G, Lenihan P, Maldonado J, Markowska-Daniel I, Pensaert M, Pospisil Z, Koch G. Seroprevalence of H1N1, H3N2, H1N2 influenza viruses in pigs in seven European countries in 2002-2003. Influenza Other Respir Viruses 2008; 2: 99-105.
  • 36 Van Reeth K, Brown IH, Brown CW. Swine influenza. Diseases of Swine. 10th Zimmerman JJ, Karriker LA, Ramirez A, Schwartz KJ, Stevenson GW. Ames (Iowa, USA): Wiley-Blackwell; 2012: 557-571.
  • 37 Wang Y, Gagnon CA, Savard C, Music N, Srednik M, Segura M, Lachance C, Bellehumeur C, Gottschalk M. Capsular sialic acid of Streptococcus suis serotype 2 binds to swine influenza virus and enhances bacterial interactions with virus-infected tracheal epithelial cells. Infect Immun 2013; 81: 4498-4508.
  • 38 Wills RW, Doster AR, Galeota JA, Sur JH, Osorio FA. Duration of infection and proportion of pigs persistently infected with porcine reproductive and respiratory syndrome virus. J Clin Microbiol 2003; 41: 58-62.
  • 39 Yazawa S, Okada M, Ono M, Fujii S, Okuda Y, Shibata I, Kida H. Experimental dual infection of pigs with an H1N1 swine influenza virus (A/Sw/Hok/2/81) and Mycoplasma hyopneumoniae . Vet Microbiol 2004; 98: 221-228.
  • 40 Zell R, Motzke S, Krumbholz A, Wutzler P, Herwig V, Dürrwald R. Novel reassortant of swine influenza H1N2 virus in Germany. J Gen Virol 2008; 89: 1-6.