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Endoscopy 2016; 48(11): 1016-1022
DOI: 10.1055/s-0042-113597
Innovations and brief communication
© Georg Thieme Verlag KG Stuttgart · New York

Successful application of endoscopic ultrasound-guided fine needle biopsy to establish pancreatic patient-derived tumor xenografts: a pilot study

Els Hermans   *
1   Laboratory of Gynecologic Oncology, Department of Oncology, University of Leuven, Leuven, Belgium
,
Schalk W. Van der Merwe   *
2   Department of Gastroenterology and Hepatology, University of Leuven, Leuven, Belgium
3   Laboratory of Hepatology, Department of Clinical and Experimental Medicine, University of Leuven, Leuven, Belgium
,
Jeroen Depreeuw
1   Laboratory of Gynecologic Oncology, Department of Oncology, University of Leuven, Leuven, Belgium
4   Laboratory for Translational Genetics, Department of Oncology, University of Leuven, Leuven, Belgium
5   VIB, Vesalius Research Center, Leuven, Belgium
,
Jeroen Dekervel
2   Department of Gastroenterology and Hepatology, University of Leuven, Leuven, Belgium
3   Laboratory of Hepatology, Department of Clinical and Experimental Medicine, University of Leuven, Leuven, Belgium
,
Enrico Radaelli
6   Center for the Biology of Disease, KU Leuven Center for Human Genetics – InfraMouse, VIB, University of Leuven, Leuven, Belgium
,
Tania Roskams
7   Division of Translational Cell & Tissue Research, Department of Imaging and Pathology, University of Leuven, Leuven, Belgium
,
Jos van Pelt Jos
2   Department of Gastroenterology and Hepatology, University of Leuven, Leuven, Belgium
,
Baki Topal
8   Department of Abdominal Surgery, University of Leuven, Leuven, Belgium
,
Chris Verslype
2   Department of Gastroenterology and Hepatology, University of Leuven, Leuven, Belgium
3   Laboratory of Hepatology, Department of Clinical and Experimental Medicine, University of Leuven, Leuven, Belgium
,
Hans Prenen
2   Department of Gastroenterology and Hepatology, University of Leuven, Leuven, Belgium
,
Werner Van Steenbergen
2   Department of Gastroenterology and Hepatology, University of Leuven, Leuven, Belgium
,
Frederik Nevens
2   Department of Gastroenterology and Hepatology, University of Leuven, Leuven, Belgium
,
Diether Lambrechts
4   Laboratory for Translational Genetics, Department of Oncology, University of Leuven, Leuven, Belgium
5   VIB, Vesalius Research Center, Leuven, Belgium
,
Frédéric Amant
1   Laboratory of Gynecologic Oncology, Department of Oncology, University of Leuven, Leuven, Belgium
9   Antoni van Leeuwenhoek, Nederlands Kanker Instituut, Centrum Gynecologische Oncologie Amsterdam (CGOA), Amsterdam, The Netherlands
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Publikationsverlauf

submitted 06. Mai 2016

accepted after revision 14. Juni 2016

Publikationsdatum:
14. September 2016 (online)

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Background and study aim: Typically, pancreatic patient-derived tumor xenografts (PDXs) are established by transplanting large tumor biopsies obtained through invasive surgery approaches into immunocompromised mice. We aimed to develop pancreatic PDXs by transplanting tumor tissue acquired by endoscopic ultrasound (EUS)-guided fine needle biopsies (FNB), assess take rates compared to surgery-derived PDXs, and demonstrate the histological and genetic resemblance to the original tumor.

Patients and methods: Biopsies of untreated pancreatic carcinoma were collected at surgery and during EUS and processed to generate PDXs.

Results: By centrifugation of FNB-derived tissue prior to engraftment, we achieved an engraftment rate of 60 % (6/10). Despite a decrease in stromal tissue, the general morphology of FNB-derived PDXs was conserved as assessed by histopathology. At the genetic level, somatic mutation and copy number profiles were largely similar to the primary tumor.

Conclusion: We show that it is technically feasible to establish pancreatic PDXs using a minimally invasive sampling technique, such as EUS-FNB. Although only a limited amount of tumor tissue was acquired, we obtained results similar to those from surgery-derived PDXs.

* These authors contributed equally.


 

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