Endoscopy 2019; 51(02): 174-178
DOI: 10.1055/a-0641-5091
Innovations and brief communications
© Georg Thieme Verlag KG Stuttgart · New York

Multiphoton imaging provides a superior optical biopsy to that of confocal laser endomicroscopy imaging for colorectal lesions

Jun Yan*
1  Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, P.R. China
2  Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education & Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fuzhou, P.R. China
,
Xiaoling Zheng*
1  Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, P.R. China
3  Fujian Provincial Hospital, Teaching Hospital of Fujian Medical University, Fuzhou, Fujian, P.R. China
,
Zhangyuanzhu Liu
1  Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, P.R. China
,
Wenju Liu
1  Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, P.R. China
4  Fujian Provincial Cancer Hospital, Teaching Hospital of Fujian Medical University, Fuzhou, Fujian, P.R. China
,
Dajia Lin
3  Fujian Provincial Hospital, Teaching Hospital of Fujian Medical University, Fuzhou, Fujian, P.R. China
,
Dexin Chen
1  Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, P.R. China
,
Kai Li
1  Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, P.R. China
,
Wei Jiang
1  Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, P.R. China
,
Zhiming Li
1  Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, P.R. China
,
Ning Zuo
2  Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education & Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fuzhou, P.R. China
,
Gang Chen
4  Fujian Provincial Cancer Hospital, Teaching Hospital of Fujian Medical University, Fuzhou, Fujian, P.R. China
,
Jianping Lu
4  Fujian Provincial Cancer Hospital, Teaching Hospital of Fujian Medical University, Fuzhou, Fujian, P.R. China
,
Jianxin Chen
2  Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education & Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fuzhou, P.R. China
,
Guoxin Li
1  Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, P.R. China
,
Chris Xu
5  School of Applied and Engineering Physics, Cornell University, Ithaca, New York, USA
,
Shuangmu Zhuo
2  Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education & Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fuzhou, P.R. China
› Author Affiliations
Further Information

Publication History

submitted 11 November 2017

accepted after revision 11 May 2018

Publication Date:
11 July 2018 (eFirst)

Abstract

Background Confocal laser endomicroscopy (CLE) requires fluorescence agents, the use of which leads to blurred images and low diagnostic accuracy owing to fluorescein leakage. We aimed to explore whether multiphoton imaging (MPI) could serve as a better method of optical biopsy.

Methods First, a pilot study was performed to set up the optical diagnostic criteria of MPI for benign or malignant colorectal lesions in 30 patients. Then, a blinded study was conducted to compare the sensitivity, specificity, and accuracy of MPI versus CLE imaging in 79 patients.

Results In the pilot study, MPI revealed regular tissue architecture and cell morphology in the normal tissue, and irregular tubular structures, and cellular and nuclear pleomorphism in the abnormal tissue. In the blinded study, compared with CLE imaging, MPI significantly improved the diagnostic sensitivity, specificity, and accuracy of the optical biopsy (89.74 % vs. 61.54 %, P = 0.008; 92.5 % vs. 67.5 %, P = 0.009; and 91.14 % vs. 64.56 %, P = 0.001, respectively).

Conclusions MPI can provide a superior optical biopsy to that of CLE imaging for colorectal lesions.

* These authors contributed equally to this work.


Table e1, e3 and e4