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CC BY 4.0 · Endosc Int Open 2025; 13: a25934172
DOI: 10.1055/a-2593-4172
Original article

Benefits of macroscopic on-site evaluation in endoscopic ultrasound-guided tissue acquisition for comprehensive genomic profiling

Authors

  • Junya Sato 

    1   Division of Endoscopy, Shizuoka Cancer Center, Sunto-gun, Japan (Ringgold ID: RIN38471)
    2   Gastroenterology, St Marianna University School of Medicine, Kawasaki, Japan (Ringgold ID: RIN12927)

  • Hirotoshi Ishiwatari 

    1   Division of Endoscopy, Shizuoka Cancer Center, Sunto-gun, Japan (Ringgold ID: RIN38471)

  • Kazuma Ishikawa

    3   Department of Medical Oncology, Sapporo Medical University, Sapporo, Japan (Ringgold ID: RIN13035)
    1   Division of Endoscopy, Shizuoka Cancer Center, Sunto-gun, Japan (Ringgold ID: RIN38471)

  • Hiroki Sakamoto

    1   Division of Endoscopy, Shizuoka Cancer Center, Sunto-gun, Japan (Ringgold ID: RIN38471)

  • Takuya Doi 

    1   Division of Endoscopy, Shizuoka Cancer Center, Sunto-gun, Japan (Ringgold ID: RIN38471)

  • Masahiro Yamamura

    1   Division of Endoscopy, Shizuoka Cancer Center, Sunto-gun, Japan (Ringgold ID: RIN38471)

  • Kazunori Takada

    1   Division of Endoscopy, Shizuoka Cancer Center, Sunto-gun, Japan (Ringgold ID: RIN38471)

  • Yoichi Yamamoto

    1   Division of Endoscopy, Shizuoka Cancer Center, Sunto-gun, Japan (Ringgold ID: RIN38471)

  • Masao Yoshida 

    1   Division of Endoscopy, Shizuoka Cancer Center, Sunto-gun, Japan (Ringgold ID: RIN38471)

  • Sayo Ito 

    1   Division of Endoscopy, Shizuoka Cancer Center, Sunto-gun, Japan (Ringgold ID: RIN38471)

  • Noboru Kawata

    1   Division of Endoscopy, Shizuoka Cancer Center, Sunto-gun, Japan (Ringgold ID: RIN38471)

  • Kenichiro Imai

    1   Division of Endoscopy, Shizuoka Cancer Center, Sunto-gun, Japan (Ringgold ID: RIN38471)

  • Kinichi Hotta

    1   Division of Endoscopy, Shizuoka Cancer Center, Sunto-gun, Japan (Ringgold ID: RIN38471)

  • Hiroyuki Ono

    1   Division of Endoscopy, Shizuoka Cancer Center, Sunto-gun, Japan (Ringgold ID: RIN38471)
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Abstract

Background and study aims

Matched therapy based on comprehensive genomic profiling is a potential treatment option for patients with inoperable pancreatic cancer; however, the optimal method for obtaining tissue samples suitable for comprehensive genomic profiling using endoscopic ultrasound-guided tissue acquisition remains unclear. This study aimed to determine the optimal endoscopic ultrasound-guided tissue acquisition method to obtain samples for comprehensive genomic profiling.

Patients and methods

This retrospective study included 86 consecutive patients with pancreatic cancer who underwent comprehensive genomic profiling using FoundationOne CDx (Foundation Medicine Inc.) and endoscopic ultrasound-guided tissue acquisition between June 2019 and January 2023. Macroscopic visible core length was measured using on-site macroscopic evaluation in all patients. Foundation Medicine Inc. reported analysis results categorized as passed (successful FoundationOne CDx), qualified, or failed. We investigated factors predicting successful FoundationOne CDx treatment.

Results

Needles sized 22, 20, and 19 gauge were used in 63, one, and 23 patients, respectively. The stylet slow-pull and suction techniques were performed in 43 and 41 patients, respectively. Median total macroscopic visible core length in the formalin-fixed paraffin-embedded blocks subjected to FoundationOne CDx was 41 mm. The success rate for FoundationOne CDx was 66%. Multiple linear regression analysis revealed that macroscopic visible core length independently affected successful FoundationOne CDx (P = 0.0019).

Conclusions

In tissue specimens obtained using endoscopic ultrasound-guided tissue acquisition, macroscopic visible core length can be associated with an appropriate sample for FoundationOne CDx.

Keywords

Endoscopic ultrasonography - Pancreas - Tissue diagnosis


Publication History

Received: 04 December 2024

Accepted after revision: 19 March 2025

Article published online:
16 May 2025

© 2025. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/).

Georg Thieme Verlag KG
Oswald-Hesse-Straße 50, 70469 Stuttgart, Germany

Bibliographical Record
Junya Sato, Hirotoshi Ishiwatari, Kazuma Ishikawa, Hiroki Sakamoto, Takuya Doi, Masahiro Yamamura, Kazunori Takada, Yoichi Yamamoto, Masao Yoshida, Sayo Ito, Noboru Kawata, Kenichiro Imai, Kinichi Hotta, Hiroyuki Ono. Benefits of macroscopic on-site evaluation in endoscopic ultrasound-guided tissue acquisition for comprehensive genomic profiling. Endosc Int Open 2025; 13: a25934172.
DOI: 10.1055/a-2593-4172
 

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