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DOI: 10.1055/s-0028-1087170
© Georg Thieme Verlag KG Stuttgart · New York
Uniaxial Cyclic Stretch Increases Glucose Uptake into C2C12 Myotubes through a Signaling Pathway Independent of Insulin-like Growth Factor I
Publication History
received 26.02.2008
accepted 04.06.2008
Publication Date:
07 October 2008 (online)
Abstract
Insulin-like growth factor I (IGF-I), an autocrine/paracrine growth factor involved in myogenesis, has rapid effects on muscle metabolism. In a manner analogous to insulin and mechanical stimuli such as stretch, IGF-I stimulates glucose transport through recruitment of glucose transporters to surface membranes in skeletal muscles. It is known that IGF-I is secreted from skeletal muscle cells in response to stretch. Therefore, we examined whether IGF-I is involved in the mechanism by which mechanical stretch regulates glucose transport using cultured C2C12 myotubes. IGF-I increased 2-deoxy-D-glucose (2-DG) uptake, and this created an additive effect with mechanical stretch, suggesting that these stimuli enhance glucose transport through different mechanisms. In fact, IGF-I-stimulated 2-DG uptake was not blocked by dantrolene (an inhibitor of Ca2+release from sarcoplasmic reticulum), whereas the stretch-stimulated effect was abolished. Conversely, the IGF-I-stimulated 2-DG uptake was prevented by phosphatidylinositol 3-kinase inhibitor wortmannin, which did not prevent the stretch-stimulated 2-DG uptake. In addition, experiments using media conditioned by stretched myotubes indicated that a mechanically induced release of locally acting autocrine/paracrine growth factors was not sufficient for induction of 2-DG uptake. Thus, our results demonstrate that mechanical stretch signaling for glucose transport is independent of the mechanism through which IGF-I increases this transport.
Key words
growth factor - skeletal muscle - autocrine/paracrine
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Correspondence
S. SuzukiPhD
Program in Physical and Occupational Therapy
Nagoya University Graduate School of Medicine
1-1-20 Daikominami
Higashi-ku
Nagoya 461-8673
Japan
Phone: +81/52/719 13 62
Fax: +81/52/719 13 62
Email: suzuki@met.nagoya-u.ac.jp