Determination of glyceryl trinitrate and its two main metabolites in human plasma using a new sensitive gas chromatography method

Mohammadreza Ghandforoush-Sattari; Parvin Zakeri-Milani; Siminozar Mashayekhi; Hadi Valizadeh

doi:10.1055/s-0031-1296180

Arzneimittelforschung, Table of Contents

Arzneimittelforschung 2011; 61(3): 141-147
DOI: 10.1055/s-0031-1296180

Cardiac Drugs · Cardiac Stimulants · Coronary Drugs

Editio Cantor Verlag Aulendorf (Germany)

Determination of glyceryl trinitrate and its two main metabolites in human plasma using a new sensitive gas chromatography method

Mohammadreza Ghandforoush-Sattari

¹Faculty of Pharmacy, Tabriz University of Medical Science, Tabriz, Iran

²Haematology and Oncology Research Center, Tabriz University of Medical Science, Tabriz, Iran

³Infectious and Tropical Diseases Research Center, Tabriz University of Medical Science, Tabriz, Iran

,

Parvin Zakeri-Milani

⁴Drug Applied Research Center, Tabriz University of Medical Science, Tabriz, Iran

¹Faculty of Pharmacy, Tabriz University of Medical Science, Tabriz, Iran

,

Siminozar Mashayekhi

¹Faculty of Pharmacy, Tabriz University of Medical Science, Tabriz, Iran

⁵National Public Health Management Centre (NPMC), Tabriz University of Medical Science, Tabriz, Iran

,

Hadi Valizadeh

⁶Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Science, Tabriz, Iran

¹Faculty of Pharmacy, Tabriz University of Medical Science, Tabriz, Iran

› Author Affiliations

Abstract

The aim of the present study was to determine the concentrations of nitroglycerin (glyceryl trinitrate, GTN, CAS 55-63-0) and its two main stable metabolites; 1,2-dinitroglycerin (1,2-glyceryl dinitrate, GDN, CAS 621-65-8) and 1,3-dinitrogly-cerin (1,3-GDN, CAS 623-87-0) in human plasma using a capillary gas chromatography method with an electron capture detection. Using the GC conditions, linear calibrations were obtained for 1,3-GDN from 0.14 to 3 ng/mL, for 1,2-GDN from 0.06 to 6 ng/mL, and for GTN from 0.01 to 0.3 ng/mL in plasma samples by the following calibration curve equations: [y = 0.1924x − 0.0088 (r² = 0.999)], [y = 0.2273x + 0.0164 (r² = 0.995)], [y = 17.434x − 0.0751] for 1,3-GDN, 1,2-GDN, and GTN respectively. The calculated limits of quantification values for GTN, 1,2-GDN, and 1,3-GDN were 0.03 ng/mL, 0.2 ng/mL, and 0.15 ng/mL respectively. This method was verified with a bioequivalence study of an Iranian brand of oral sustained release nitroglycerin with an innovator formulation.

Key words

CAS 55-63-0 - Gas chromatography - Glyceryl trinitrate - Bioequivalence - Nitroglycerin - Pharmacokinetics

Full Text

References

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