Synlett 2012; 23(12): 1840-1842
DOI: 10.1055/s-0032-1316554
letter
© Georg Thieme Verlag Stuttgart · New York

Monitoring Biocatalytic Transformations Mediated by Polyketide Synthase Enzymes in Cell Lysate via Fluorine NMR

Shawn K. Piasecki
a  Institute for Cellular and Molecular Biology, The University of Texas at Austin, 1 University Station A5300, Austin, TX 78712, USA
,
Adrian T. Keatinge-Clay*
a  Institute for Cellular and Molecular Biology, The University of Texas at Austin, 1 University Station A5300, Austin, TX 78712, USA
b  Department of Chemistry and Biochemistry, The University of Texas at Austin, 1 University Station A5300, Austin, TX 78712, USA, Fax: +1(512)4718696   Email: adriankc@mail.utexas.edu
› Author Affiliations
Further Information

Publication History

Received: 12 April 2012

Accepted after revision: 25 May 2012

Publication Date:
04 July 2012 (online)


Abstract

The biocatalytic employment of modular polyketide synthase enzymes in cell lysate has become a viable route to preparative quantities of synthetically valuable polyketide fragments. We report the quantitative, uninvasive, and continuous monitoring of such biocatalytic reactions by observing trifluoromethyl-bearing substrates via 19F NMR spectroscopic analysis. To demonstrate the utility of this technique, we followed reactions catalyzed by a thioesterase and several ketoreductases.

Supporting Information

 
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