PDF herunterladen
Drug Res (Stuttg) 2014; 64(6): 287-295
DOI: 10.1055/s-0033-1357192
Original Article
© Georg Thieme Verlag KG Stuttgart · New York

To Investigate Antihyperglycemic and Antihyperlipidemic Potential of Safrole in Rodents by in-vivo and in-vitro Study

S. Rani
1   Division of Pharmacology, Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Haryana, India
,
S. Sharma
1   Division of Pharmacology, Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Haryana, India
,
S. Kumar
1   Division of Pharmacology, Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Haryana, India
› Institutsangaben
Weitere Informationen

Publikationsverlauf

received 12. September 2013

accepted 22. September 2013

Publikationsdatum:
16. Oktober 2013 (online)

Auch verfügbar auf
    Lizenzen und Reprints

Abstract

In the present investigation anti-diabetic and in-vitro antioxidant potential of safrole were evaluated (100 and 200 mg/kg p.o.) in acute and chronic Streptozotocin-nicotinamide (STZ) induced antihyperglycemic rat model. The oral administration of safrole for 30 days affects the level of blood glucose, glycosylated hemoglobin (HbA1C), total cholesterol (TC), triglycerides (TG), phospholipids, high density lipoprotein (HDL), body weight, insulin level, liver glycogen content, antioxidant parameters, lipase, α-amylase in normal and STZ induced diabetic rats. The oral administration of safrole at dose 100 & 200 mg/kg p.o. significantly improve the diabetic condition in Streptozotocin-induced diabetic rats. In enzymatic assay, the IC50 value of the safrole for α-amylase and lipase was found to be 702.78 and 861.35 μg/ml respectively which was found comparable with the standard drug (ascorbic acid) as 252.12 μg/ml. Further studies can be performed on safrole for mechanistic and toxicological aspects so that it can be investigated as a new substance for the management of various diseases.

Key words

antihyperglycemic activity - streptozotocin - safrole - lipase - amylase
 

  • References

  • 1 Rother KI. Diabetes treatment – Bridging the Divide. N Engl J Med 356: 1499-1501
    PubMedGoogle Scholar
  • 2 Betteridge J. Lipid disorders in diabetes mellitus. In: Pickup JC, Williams G. (eds.) Text Book of Diabetes. Blackwell Science Publishers; London: 1997: 1-35
    Google Scholar
  • 3 Xu H, Blair NT, Clapham DE. Camphor activates and strongly desensitizes the transient receptor potential vanilloid subtype 1 channel in a vanilloid-independent mechanism. J Neur 2005; 25: 8924-8937
    PubMedGoogle Scholar
  • 4 Jochen GW, Theis MD. Camphorated oil: still endangering the lives of Canadian children. Canadian Medical Association journal 1995; 152: 1821-1824
    PubMedGoogle Scholar
  • 5 Inoue Y, Takeuchi S. Expectorant-like action of camphor derivatives. Nippon Ika Daigaku Zasshi 1969; 36: 351-354
    PubMedGoogle Scholar
  • 6 Guilland-Cumming DF, Smith GJ. The effect of camphor on mitochondrial respiration. Experientia 1982; 38: 236-237
    CrossrefPubMedGoogle Scholar
  • 7 Goel HC, Roa AR. Radio sensitizing effect of camphor on transplantable mammary adenocarcinoma in mice. Canc Lett 1988; 43: 21-27
    CrossrefPubMedGoogle Scholar
  • 8 Akram J, Javed S, Ali AS et al. Effects of camphor oil on sexual behaviour in male rats. Iranian journal of pharmaceutical sciences 2006; 2: 209-214
    PubMedGoogle Scholar
  • 9 Morsy TA, Rahem MA, El-Sharkawy EM et al. Eucalyptus globulus (camphor oil) against the zoonotic scabies, Sarcoptes scabiei. J Egypt Soc Parasit 2003; 33: 47-53
    PubMedGoogle Scholar
  • 10 Sudha P, Smita SZ, Shobha YB et al. Potent α-amylase inhibitory activity of Indian Ayurvedic medicinal plants. BMC Compl Alt Med 2011; 11: 5
    CrossrefPubMedGoogle Scholar
  • 11 Mukherjee M. Human digestive and metabolic lipases – a brief review. J Mol Catal B: Enzym 2003; 22: 369-376
    CrossrefPubMedGoogle Scholar
  • 12 Marudamuthu AS, Leelavinothan P. Effect of pterostilbene on lipids and lipid profiles in Streptozotocin – Nicotinamide induced type 2 diabetes mellitus. J Appl Biomed 2008; 6: 31-37
    PubMedGoogle Scholar
  • 13 Kumar J, Sharma S, Kumar S. In-vivo anti-diabetic and antioxidant potential of Psoralea corylifolia seeds in STZ induced type-2 diabetic rats. J health sci 2011; 57: 225-235
    CrossrefPubMedGoogle Scholar
  • 14 Kumar S, Vasudena N, Sharma S. GC-MS analysis and screening of antidiabetic, antioxidant and hypolipidemic potential of Cinnamomum tamala oil in streptozotocin induced diabetes mellitus in rats. Cardiovas Diabetol 2012; 11: 95
    PubMedGoogle Scholar
  • 15 Sedlak J, Lindsay RH. Estimation of total protein bound and non protein bound sulfhydryl group in tissue with ellman’s reagent. Anal Biochem 1968; 15: 192-205
    PubMedGoogle Scholar
  • 16 Scifter S, Dayton S, Molic B et al. The estimation of glycogen with the anthrone reagent. In Archive Biochem 1950; 25: 191
    PubMedGoogle Scholar
  • 17 Kumar S, Singh R, Vasudeva N et al. Acute and chronic animal models for the evaluation of anti-diabetic agents. Cardiovas Diabetol 2012; 11: 9
    PubMedGoogle Scholar
  • 18 Xiao Z, Storms R, Tsang A. A quantitative starch-iodine method for measuring alpha-amylase and glucoamylase activities. Anal Biochem 2006; 351: 146-148
    CrossrefPubMedGoogle Scholar
  • 19 Yoshikawa M, Shimoda H, Nishida N et al. Salacia reticulata and its polyphenolic constituents with lipase inhibitory and lipolytic activities have mild anti-obesity effects in rats. J Nutr 2002; 132: 1819-1824
    PubMedGoogle Scholar
  • 20 Han LK, Kimura Y, Okuda H. Reduction in fat storage during chitin-chitosan treatment in mice fed a high-fat diet. Int J Obes 1999; 23: 174-179
    CrossrefPubMedGoogle Scholar
  • 21 Egesie UG, Adelaiye AB, Ibu JO et al. Safety and hypoglycemic properties of aqueous leave extract of Ocimum gratissium in streptozotocin induced diabetic rabbits. Nig J Physiol Sci 2006; 21: 31-35
    PubMedGoogle Scholar
  • 22 Ahmed I, Cummings E, Adeghate E et al. Beneficial effects and mechanism of action of Momordica charantia fruit juice in the treatment of streptozotocin-induced diabetes mellitus in rats. Mol Cell Biochem 2004; 26: 63-70
    PubMedGoogle Scholar
  • 23 Sarkar S, Pranava M, Manita R. Demonstration of the hypoglycemic action of Momordica charantia in a validated animal model of diabetes. Pharmacological Research 2007; 33: 1-4
    PubMedGoogle Scholar
  • 24 Karunanayake EH, Jeevathayaparan S, Tennekoon KH. Effect of Momordica charantia fruit juice on streptozotocin-induced diabetes in rats. J Ethnopharmacol 1990; 30: 199-204
    CrossrefPubMedGoogle Scholar
  • 25 Bailey CJ, Day C, Turner SL et al. Cerasee, a traditional treatment for diabetes. Studies in normal and streptozotocin diabetic mice. Diabetes Res 1985; 2: 81-84
    PubMedGoogle Scholar
  • 26 Betteridge J. Lipid disorders in diabetes mellitus. In: Pickup JC, Williams G. (eds.) Text Book of Diabetes. Blackwell Science Publishers; London: 1997: 1-35
    Google Scholar
  • 27 Taskinen M. Quantitative and qualitative lipoprotein abnormalities in diabetes mellitus. Diabetes 1992; 41: 12-17
    PubMedGoogle Scholar
  • 28 Giugliano D, Ceriello A, Paolisso G. Oxidative stress and diabetic vascular level and the P.E. Fraction showed a moderate increase in GSHcomplications. Diab Car 1996; 19: 257-267
    PubMedGoogle Scholar
  • 29 Song-Hwan B, Hyung-Joo S. Antioxidant activities of five different mulberry cultivars in Korea. LWT 2007; 40: 955-962
    CrossrefPubMedGoogle Scholar
  • 30 Pavana P, Sethupathy S, Santa K et al. Effects of Tephrosia purpurea aqueous seed extract on blood glucose and antioxidant enzyme activities in streptozotocin induced diabetic rats. Afr J Trad CAM 2009; 6: 78-86
    PubMedGoogle Scholar
  • 31 Gallaher D, Schneeman BO. Nutritional and metabolic response to plant inhibitors of digestive enzymes. Adv Exp Med Biol 1986; 199: 167-184
    PubMedGoogle Scholar
  • 32 Yasser B, Mohammad M, Mohammad H et al. Screening of Some Medicinal Plants for their Pancreatic Lipase Inhibitory Potential. Jord J Pharm Sci 2011; 4: 81
    PubMedGoogle Scholar