Measurement of Serum Osteopontin and Interleukin-17 in Asthmatic Children in Saudi Arabia

Ali Khairallah Alzahrani; Nader Mohammed Mohammed; Humaid Al-Swat; Rehab Ahmed Karam

doi:10.1055/s-0035-1554779

Journal of Pediatric Biochemistry, Table of Contents

Journal of Pediatric Biochemistry 2015; 05(01): 034-037
DOI: 10.1055/s-0035-1554779

Original Article

Georg Thieme Verlag KG Stuttgart · New York

Measurement of Serum Osteopontin and Interleukin-17 in Asthmatic Children in Saudi Arabia

Authors

Ali Khairallah Alzahrani

¹Department of Paediatrics and Neonatology, Taif University, Al Taif, Saudi Arabia
Nader Mohammed Mohammed

¹Department of Paediatrics and Neonatology, Taif University, Al Taif, Saudi Arabia

²Department of Paediatrics, Zagazig General Hospital, Zagazig, Egypt
Humaid Al-Swat

³Children Hospital, Al Taif, Saudi Arabia
Rehab Ahmed Karam

⁴Department of Biochemistry, Taif University, Al Taif, Saudi Arabia

⁵Department of Biochemistry, Zagazig University, Zagazig, Egypt

Abstract

Cytokines play an important role in the pathophysiology of asthma by affecting airway remodeling and allergic reactions. We aimed to measure the serum concentrations of osteopontin (OPN) and interleukin (IL)-17 in a group of asthmatic and normal children to test the association of these two cytokines with the severity of asthma in Saudi children. Our study included 50 asthmatic and 50 normal children. Serum cytokines were measured by ELISA technique. Pulmonary function tests were performed in the asthmatic children. Serum levels of OPN and IL-17 were significantly increased in asthmatic children in comparison to control group. Also, the concentrations of OPN and IL-17 were significantly associated with asthma severity. There was a significant positive correlation between OPN and IL-17 levels. In conclusion, our study suggests that OPN and IL-17 may play a role in childhood asthma and are strongly related to the disease severity.

Keywords

interleukin-17 - osteopontin - bronchial asthma

Full Text

References

Reference
1 Al Frayh AR, Shakoor Z, Gad El Rab MO, Hasnain SM. Increased prevalence of asthma in Saudi Arabia. Ann Allergy Asthma Immunol 2001; 86 (3) 292-296
2 Broide DH. Immunologic and inflammatory mechanisms that drive asthma progression to remodeling. J Allergy Clin Immunol 2008; 121 (3) 560-570 , quiz 571–572
3 O'Regan A. The role of osteopontin in lung disease. Cytokine Growth Factor Rev 2003; 14 (6) 479-488
4 Ashkar S, Weber GF, Panoutsakopoulou V , et al. Eta-1 (osteopontin): an early component of type-1 (cell-mediated) immunity. Science 2000; 287 (5454) 860-864
5 Konno S, Kurokawa M, Uede T, Nishimura M, Huang SK. Role of osteopontin, a multifunctional protein, in allergy and asthma. Clin Exp Allergy 2011; 41 (10) 1360-1366
6 Xanthou G, Alissafi T, Semitekolou M , et al. Osteopontin has a crucial role in allergic airway disease through regulation of dendritic cell subsets. Nat Med 2007; 13 (5) 570-578
7 Kohan M, Bader R, Puxeddu I, Levi-Schaffer F, Breuer R, Berkman N. Enhanced osteopontin expression in a murine model of allergen-induced airway remodelling. Clin Exp Allergy 2007; 37 (10) 1444-1454
8 Takahashi A, Kurokawa M, Konno S , et al. Osteopontin is involved in migration of eosinophils in asthma. Clin Exp Allergy 2009; 39 (8) 1152-1159
9 Louten J, Boniface K, de Waal Malefyt R. Development and function of TH17 cells in health and disease. J Allergy Clin Immunol 2009; 123 (5) 1004-1011
10 Bullens DM, Truyen E, Coteur L , et al. IL-17 mRNA in sputum of asthmatic patients: linking T cell driven inflammation and granulocytic influx?. Respir Res 2006; 7: 135
11 Wang YH, Wills-Karp M. The potential role of interleukin-17 in severe asthma. Curr Allergy Asthma Rep 2011; 11 (5) 388-394
12 American Thoracic Society. Standards for the diagnosis and care of patients with chronic obstructive pulmonary disease (COPD) and asthma, November 1986. Am Rev Respir Dis 1987; 136 (1) 225-244
13 National Heart, Lung, and Blood Institute (US) National Asthma Education and Prevention Program Expert Panel Report 3: Guidelines for the Diagnosis and Management of Asthma. NIH Publication 2007; Number 08-5846
14 Fagerland MW, Sandvik L. The Wilcoxon-Mann-Whitney test under scrutiny. Stat Med 2009; 28 (10) 1487-1497
15 Denhardt DT, Noda M, O'Regan AW, Pavlin D, Berman JS. Osteopontin as a means to cope with environmental insults: regulation of inflammation, tissue remodeling, and cell survival. J Clin Invest 2001; 107 (9) 1055-1061
16 Frenzel DF, Weiss JM. Osteopontin and allergic disease: pathophysiology and implications for diagnostics and therapy. Expert Rev Clin Immunol 2011; 7 (1) 93-109
17 Annunziato F, Cosmi L, Liotta F, Maggi E, Romagnani S. Type 17 T helper cells—origins, features and possible roles in rheumatic disease. Nat Rev Rheumatol 2009; 5 (6) 325-331
18 Pernis AB. Th17 cells in rheumatoid arthritis and systemic lupus erythematosus. J Intern Med 2009; 265 (6) 644-652
19 Akelma AZ, Cizmeci MN, Kanburoglu MK , et al. Elevated level of serum osteopontin in school-age children with asthma. Allergol Immunopathol (Madr) 2014; 42 (4) 275-281
20 Simoes DC, Xanthou G, Petrochilou K, Panoutsakopoulou V, Roussos C, Gratziou C. Osteopontin deficiency protects against airway remodeling and hyperresponsiveness in chronic asthma. Am J Respir Crit Care Med 2009; 179 (10) 894-902
21 Samitas K, Zervas E, Vittorakis S , et al. Osteopontin expression and relation to disease severity in human asthma. Eur Respir J 2011; 37 (2) 331-341
22 Zhao JJ, Yang L, Zhao FQ, Shi SM, Tan P. Osteopontin levels are elevated in patients with asthma. J Int Med Res 2011; 39 (4) 1402-1407
23 Delimpoura V, Bakakos P, Tseliou E , et al. Increased levels of osteopontin in sputum supernatant in severe refractory asthma. Thorax 2010; 65 (9) 782-786
24 Yao Z, Fanslow WC, Seldin MF , et al. Herpesvirus Saimiri encodes a new cytokine, IL-17, which binds to a novel cytokine receptor. Immunity 1995; 3 (6) 811-821
25 Molet S, Hamid Q, Davoine F , et al. IL-17 is increased in asthmatic airways and induces human bronchial fibroblasts to produce cytokines. J Allergy Clin Immunol 2001; 108 (3) 430-438
26 Hellings PW, Kasran A, Liu Z , et al. Interleukin-17 orchestrates the granulocyte influx into airways after allergen inhalation in a mouse model of allergic asthma. Am J Respir Cell Mol Biol 2003; 28 (1) 42-50
27 Wang YH, Liu YJ. The IL-17 cytokine family and their role in allergic inflammation. Curr Opin Immunol 2008; 20 (6) 697-702
28 Chakir J, Shannon J, Molet S , et al. Airway remodeling-associated mediators in moderate to severe asthma: effect of steroids on TGF-β, IL-11, IL-17, and type I and type III collagen expression. J Allergy Clin Immunol 2003; 111 (6) 1293-1298
29 Shinohara ML, Kim JH, Garcia VA, Cantor H. Engagement of the type I interferon receptor on dendritic cells inhibits T helper 17 cell development: role of intracellular osteopontin. Immunity 2008; 29 (1) 68-78
30 Chen M, Chen G, Nie H , et al. Regulatory effects of IFN-beta on production of osteopontin and IL-17 by CD4+ T Cells in MS. Eur J Immunol 2009; 39 (9) 2525-2536
31 Ivanov S, Lindén A. Interleukin-17 as a drug target in human disease. Trends Pharmacol Sci 2009; 30 (2) 95-103
32 Park SJ, Lee YC. Interleukin-17 regulation: an attractive therapeutic approach for asthma. Respir Res 2010; 11: 78