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Journal of Pediatric Biochemistry 2014; 04(03): 153-157
DOI: 10.1055/s-0036-1586475
Review Article
Georg Thieme Verlag KG Stuttgart – New York

Measurement of troponin-T in dried blood spots and dried plasma spots: A pilot study

Yotam Almagor
a   Department of Neonatology, Hadassah and Hebrew University Hospital, Jerusalem, Israel
,
Smadar Eventov-Friedman
a   Department of Neonatology, Hadassah and Hebrew University Hospital, Jerusalem, Israel
,
Amiram Nir
b   Pediatric Cardiology Unit, Hadassah and Hebrew University Hospital, Jerusalem, Israel
,
Awad Sror
c   Central Clinical Laboratory, Hadassah and Hebrew University Hospital, Jerusalem, Israel
,
Ann Saada
d   Monique and Jacques Roboh Department of Genetic Research, and Department of Genetics and Metabolic Diseases, Hadassah and Hebrew University Hospital, Jerusalem, Israel
› Author Affiliations
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Publication History

07 July 2013

23 September 2013

Publication Date:
03 August 2016 (online)

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Abstract

Troponin-T (TnT) is a biomarker for cardiac insult, but its significance as a cardiac biomarker in neonates is unclear. A method for measuring TnT in dried blood spots (DBS), which are used worldwide for neonatal screening, will enable access to a very large potential database. We tested concentrations of TnT in dried blood and dried plasma and compared results to the TnT concentrations in the original fresh blood. Troponin results in dried blood were significantly changed and did not allow sufficient test resolution. In contrast, troponin concentrations in dried plasma correlated well to those of all original samples tested. Our preliminary results suggest that TnT can accurately be measured in dried plasma, but TnT concentrations in dried blood are detectable only if troponin values are significantly elevated.

Keywords

troponin - TnT - dried blood spots - dried plasma spots - Guthrie