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Thromb Haemost 2003; 89(02): 235-242
DOI: 10.1055/s-0037-1613437
Blood Coagulation, Fibrinolysis and Cellular Haemostasis
Schattauer GmbH

Effect of factor VIII on tissue factor-initiated spatial clot growth

Mikhail V. Ovanesov
1   Laboratory of Physical Biochemistry of Blood, Russian Academy of Medical Sciences, Moscow, Russia
,
Elena G. Lopatina
2   Haemophilia Center, Research Center for Hematology, Russian Academy of Medical Sciences, Moscow, Russia
,
Evgueni L. Saenko
3   Jerome H. Holland Laboratory for the Biomedical Sciences, American Red Cross, Rockville, Maryland, USA
4   George Washington University School of Medicine, Washington, District of Columbia, USA
,
Natalya M. Ananyeva
3   Jerome H. Holland Laboratory for the Biomedical Sciences, American Red Cross, Rockville, Maryland, USA
,
Ljudmila I. Ul’yanova
5   Department of Genetics and Histology, Institute of Immunology, Ministry of Health, Moscow, Russia
,
Olga P. Plyushch
2   Haemophilia Center, Research Center for Hematology, Russian Academy of Medical Sciences, Moscow, Russia
,
Andrey A. Butilin
6   Moscow State University, Moscow, Russia
,
Fazly I. Ataullakhanov
1   Laboratory of Physical Biochemistry of Blood, Russian Academy of Medical Sciences, Moscow, Russia
6   Moscow State University, Moscow, Russia
› Author Affiliations
Further Information

Publication History

Received 04 March 2002

Accepted after resubmission 07 November 2002

Publication Date:
07 December 2017 (online)

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Summary

Using time-lapse videomicroscopy, we studied the role of coagulation factor VIII (fVIII) in tissue factor-initiated spatial clot growth on fibroblast monolayers in a thin layer of non-stirred recalcified plasma from healthy donors or patients with severe Haemophilia A. Analysis of temporal evolution of light-scattering profiles from a growing clot revealed existence of two phases in the clot growth-initiation phase in a narrow (0.2 mm) zone adjacent to activator surface and elongation phase in plasma volume. While the initiation phase did not differ in normal and haemophilic plasmas, the rate of clot growth in the elongation phase in haemophilic plasma constituted only 30% of that in normal plasma. Supplementation of haemophilic plasma with 0.05 U/ml fVIII restored the normal clot growth rate (44.9 ± 2.5 μm/min) at high but not at low fibroblast density. Our results indicate that the functioning of the intrinsic tenase complex is critical for normal spatial clot growth.

Keywords

Factor VIII - Haemophilia A - spatial clot growth - fibroblast - time-lapse light-scattering videomicroscopy
 

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