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Thromb Haemost 1998; 79(01): 186-194
DOI: 10.1055/s-0037-1614248
Review Article
Schattauer GmbH

Expression of Thrombospondin 1 on the Surface of Activated Platelets Mediates their Interaction with the Heavy Chains of Human Kininogens through Lys 244-Pro 254

Raul A. DeLa Cadena
1   From the Sol Sherry Thrombosis Research Center, Philadelphia, USA
2   From the Department of Pathology and Laboratory Medicine, Philadelphia, USA
,
Satya P. Kunapuli
1   From the Sol Sherry Thrombosis Research Center, Philadelphia, USA
4   From the Department of Physiology, Temple University School of Medicine, Philadelphia, USA
,
Daniel A. Walz
5   From the Department of Physiology, Wayne State University School of Medicine, Detroit, USA
,
Robert W. Colman
1   From the Sol Sherry Thrombosis Research Center, Philadelphia, USA
3   From the Department of Medicine, Philadelphia, USA
4   From the Department of Physiology, Temple University School of Medicine, Philadelphia, USA
› Author Affiliations
Further Information

Publication History

Received 26 February 1997

Accepted after resubmission 03 September 1997

Publication Date:
08 December 2017 (online)

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Summary

Platelet thrombospondin (TSP1) forms a complex with high (HK) and low (LK) molecular weight kininogens. We isolated a proteolytic fragment from HK and LK heavy chains (12 kDa) recognized by TSP1 with a N-terminal sequence, K244ICVGCPRDIP254. Lys244-Pro254 oxidized to cyclic form prevented binding of 125I-LK to TSP1. This effect was abolished by reduction and alkylation. Oxidized peptide KICVGCPRDIP (100 μM) reversed the known inhibitory effects of LK or HK (1 μM), on thrombin-induced platelet activation, suggesting this peptide forms part of the cell binding site on HK and LK for activated platelets. KICVGCPRDIP completely inhibited the binding of 125I-LK to activated platelets. However, the peptide only partially inhibited binding of 125I-HK to platelets, suggesting an additional binding site on the HK light chain. Fluorescein-labeled KICVGCPRDIP bound directly and specifically to activated platelets. A monoclonal antibody directed to TSP1 partially inhibited the binding of 125I-HK to activated but not inactivated platelets. We conclude residues Lys244-Pro254 on kininogen heavy chain is responsible for binding to thrombospondin on the surface of activated platelets.

 

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