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Nuklearmedizin 1969; 08(02): 196-206
DOI: 10.1055/s-0038-1624672
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Einfluß der Glucose auf die [14C] Thymidin-Einbaurate von Ehrlich-Ascitescarcinomzellen in vitro

Influence of Glucose on the Incorporation of [14C] Thymidine in Ehrlich Ascites-Carcinoma Cells in VitroL’influence de la glucose sur le taux de fixation de la 14C-Thymidine dans les cellules de carcinome d’Ehrlich in vitro
Dieter. Kummer
1   Aus der Chirurgischen Klinik der Universität Tübingen (Direktor: Prof. Dr. L. Koslowski)
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Eingegangen am: 15 January 1969

Publication Date:
22 January 2018 (online)

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Zusammenfassung

In nahezu glucosefreier Suspension von Ehrlich-Ascitescarcinomzellen bewirkt die Zufuhr von Glucose 2,5 × 10–4 bis 10–2 M:

1. Hemmung der [14C] Thymidin-Einbaurate in die Zellen.

2. Aktivierung des Ribonucleotid-Reductase-Systems und damit Stimulierung der Desoxyribonucleotidsynthese (auch der Thymidintriphosphat-de-novo-Synthese).

3. Blockierung der Thymidinkinase über Endprodukthemmung, wodurch die Minderung des [14C] Thymidin-Einbaus in die Zellen erklärbar ist.

A glucose free medium was employed to study the effect of additional glucose 2.5 × 10–4 to 10–2 M on Ehrlich ascites-carcinoma cells:

1. The incorporation of [14C] thymidine in ascites-cells was inhibited.

2. Ribonucleotide reductase activity was stimulated, which would indicate an enhanced deoxyribonucleotide synthesis.

3. Thymidine kinase was blocked over the end-products suggesting a diminished [14C] thymidine incorporation into the cells.

Dans une suspension de cellules de carcinome d’Ehrlich libre de glucose une adition de glucose a les effects suivants.

1. Réduction du taux de fixation de Thymidine dans les cellules.

2. Activation du système de Reductase de Ribonucleotide, une stimulation de la synthèse de Desoxiribonucléotide.

3. Blocquage de la cinase de Thymidine par inhibition du produit finale, ce qui explique la diminution de la fixation de Thymidine dans les cellules.

 

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