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Methods Inf Med 2007; 46(02): 206-211
DOI: 10.1055/s-0038-1625408
Original Article
Schattauer GmbH

New Methods for Time-resolved Fluorescence Spectroscopy Data Analysis Based on the Laguerre Expansion Technique

Applications in Tissue Diagnosis
J. A. Jo
1   Department of Biomedical Engineering, Texas A&M University, College Station, TX, USA
,
L. Marcu
2   Department of Biomedical Engineering, University of California Davis, Davis, California, USA
,
Q. Fang
3   Department of Engineering Physics, McMaster University, Hamilton, Ontario, Canada
,
T. Papaioannou
4   Department of Surgery, Cedars-Sinai Medical Center, Los Angeles, California, USA
,
J. H. Qiao
5   Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, USA
,
M. C. Fishbein
5   Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, USA
,
B. Beseth
6   Department of Surgery, Division of Vascular Surgery, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, USA
,
A. H. Dorafshar
6   Department of Surgery, Division of Vascular Surgery, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, USA
,
T. Reil
6   Department of Surgery, Division of Vascular Surgery, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, USA
,
D. Baker
6   Department of Surgery, Division of Vascular Surgery, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, USA
,
J. Freischlag
7   Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
› Author Affiliations
Further Information

Publication History

Publication Date:
11 January 2018 (online)

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Summary

Objectives : A new deconvolution method for the analysis of time-resolved laser-induced fluorescence spectroscopy (TR-LIFS) data is introduced and applied for tissue diagnosis.

Method : The intrinsic TR-LIFS decays are expanded on a Laguerre basis, and the computed Laguerre expansion coefficients (LEC) are used to characterize the sample fluorescence emission. The method was applied for the diagnosis of atherosclerotic vulnerable plaques.

Results : At a first stage, using a rabbit atherosclerotic model, 73 TR-LIFS in-vivo measurements from the normal and atherosclerotic aorta segments of eight rabbits were taken. The Laguerre deconvolution technique was able to accurately deconvolve the TR-LIFS measurements. More interesting, the LEC reflected the changes in the arterial biochemical composition and provided discrimination of lesions rich in macrophages/foamcells with high sensitivity (> 85%) and specificity (> 95%). At a second stage, 348 TR-LIFS measurements were obtained from the explanted carotid arteries of 30 patients. Lesions with significant inflammatory cells (macrophages/foam-cells and lymphocytes) were detected with high sensitivity (> 80%) and specificity (> 90%), using LEC-based classifiers.

Conclusion : This study has demonstrated the potential of using TR-LIFS information by means of LEC for in- vivo tissue diagnosis, and specifically for detecting inflammation in atherosclerotic lesions, a key marker of plaque vulnerability.

Keywords

Time-resolved fluorescence spectroscopy - Laguerre deconvolution - atherosclerosis