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Thromb Haemost 1989; 61(02): 259-261
DOI: 10.1055/s-0038-1646571
Original Article
Schattauer GmbH Stuttgart

Monoclonal Antibodies to Tissue-Type Plasminogen Activator which Prolong its Clearance In Vivo

Authors

  • Thomas M Reilly

    The E. I. du Pont de Nemours and Company, Medical Products Department, Experimental Station, Wilmington, Delaware, USA

  • Robert M Knabb

    The E. I. du Pont de Nemours and Company, Medical Products Department, Experimental Station, Wilmington, Delaware, USA

  • Andrew T Chiu

    The E. I. du Pont de Nemours and Company, Medical Products Department, Experimental Station, Wilmington, Delaware, USA

  • David L Bradfute

    The E. I. du Pont de Nemours and Company, Medical Products Department, Experimental Station, Wilmington, Delaware, USA

  • Pieter B M W M Timmermans

    The E. I. du Pont de Nemours and Company, Medical Products Department, Experimental Station, Wilmington, Delaware, USA
Further Information

Publication History

Received 16 September 1988

Accepted after revision 06 December 1988

Publication Date:
30 June 2018 (online)

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Summary

Three murine monoclonal antibodies to tissue-type plasminogen activator (t-PA) were evaluated for their effects on the binding of iodinated t-PA to cultured human hepatoma cells (Hep G2), and on extending the half-life of t-PA injected into rabbits. Two of the antibodies, AE5 and EG2, significantly inhibited t-PA binding in vitro, and extended the in vivo half-life of t-PA four to five-fold. A third antibody, BA10, which had a much smaller inhibitory effect on t-PA binding, had no influence in extending t-PA’s half-life. MOPC-21, a control antibody not directed to t-PA, had no effect on either test. Our results are the first to correlate different compounds’ effects on t-PA binding with their ability to retard t-PA clearance in vivo, and provide additional evidence for the importance of a liver cell receptor in the t-PA clearance process.

Keywords

Tissue-type plasminogen activator - Hep G2 human hepatoma cells