Thromb Haemost 1989; 61(03): 448-453
DOI: 10.1055/s-0038-1646612
Original Article
Schattauer GmbH Stuttgart

Isolation and Characterization of Human Blood Platelet mRNA and Construction of a cDNA Library in λgt11

Confirmation of the Platelet Derivation by Identification of GPIb Coding mRNA and Cloning of a GPIb Coding cDNA Insert
Andreas N Wicki
*   Theodor Kocher Institute, Bern, Switzerland
,
Alfred Walz
*   Theodor Kocher Institute, Bern, Switzerland
,
Susan N Gerber-Huber
**   Institut für Biochemie und Molekularbiologie, Bern, Switzerland
,
Roland H Wenger
*   Theodor Kocher Institute, Bern, Switzerland
,
Rolf Vornhagen
***   Biotest Serum GmbH, Offenbach, FRG
,
Kenneth J Clemetson
*   Theodor Kocher Institute, Bern, Switzerland
› Author Affiliations
Further Information

Publication History

Received 26 September 1988

Accepted after revision 18 January 1989

Publication Date:
24 July 2018 (online)

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Summary

We have developed a purification method for the isolation of platelet-specific poly (A+) RNA and demonstrated that human blood platelets, despite the absence of a nucleus, contain stable mRNA. The poly (A+) RNA was used to construct a platelet- specific cDNA expression library in λgtll. The platelet derivation of the purified mRNA was confirmed by identification of membrane glycoprotein Ib (GPIb) message by immunoprecipitation of rabbit reticulocyte lysate translation products with poly- and monoclonal antibodies against GPIbα and by sequencing of a GPIbα cDNA clone