Thromb Haemost 1997; 78(03): 1138-1141
DOI: 10.1055/s-0038-1657700
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Tissue Factor Pathway Inhibitor Inhibits Aortic Smooth Muscle Cell Migration Induced by Tissue Factor/Factor VIIa Complex

Yuichiro Sato
1   The First Department of Pathology, Miyazaki Medical College, Miyazaki, Japan
,
Yujiro Asada
1   The First Department of Pathology, Miyazaki Medical College, Miyazaki, Japan
,
Kousuke Marutsuka
1   The First Department of Pathology, Miyazaki Medical College, Miyazaki, Japan
,
Kinta Hatakeyama
2   The Chemo-Therapeutic Research Institute, Kumamoto, Japan
,
Yuichi Kamikubo
2   The Chemo-Therapeutic Research Institute, Kumamoto, Japan
,
Akinobu Sumiyoshi
1   The First Department of Pathology, Miyazaki Medical College, Miyazaki, Japan
› Author Affiliations
Further Information

Publication History

Received 11 1996

Accepted after revision 22 April 1997

Publication Date:
30 July 2018 (online)

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Summary

Tissue factor (TF), a transmembrane glycoprotein, forms a high affinity complex with factor Vll/VIIa (FVIIa) and thereby initiates blood coagulation. Tissue factor pathway inhibitor (TFPI) is an endogenous protease inhibitor of TF/FVIIa-initiated coagulation. We previously reported that TF was a strong chemotactic factor for cultured vascular smooth muscle cells (SMCs). In this study, we examined the contribution of FVIIa and the effect of TFPI to TF-induced cultured SMC migration. TF/FVIIa complex showed a strong migration ability, however, neither TF alone nor FVIIa induced SMC migration. TF/FVIIa treated by a serine protease inhibitor and the complex of TF and inactivated FVIIa (DEGR-FVIIa) did not stimulate SMC migration. Pretreatment with hirudin and the antibodies to a-thrombin and factor X had no effect on TF/FVIIa-induced SMC migration, although a-thrombin and factor Xa also induced SMC migration respectively. TFPI markedly inhibited TF/FVIIa-induced SMC migration in a concentration-dependent manner, but did not affect the SMC migration induced by platelet-derived growth factor (PDGF)-BB, basic fibro blast-growth factor (bFGF), or a-thrombin. These results indicate that the catalytic activity of TF/FVIIa complex is important on SMC migration, and TFPI can reduce SMC migration as well as thrombosis.