Endoscopy 2019; 51(04): S60
DOI: 10.1055/s-0039-1681346
ESGE Days 2019 oral presentations
Friday, April 5, 2019 14:30 – 16:30: EUS therapeutic pancreas South Hall 2B
Georg Thieme Verlag KG Stuttgart · New York

FEASIBILITY OF A THREE-DIMENSIONAL SPHEROID CULTURE USING ENDOSCOPIC ULTRASOUND-GUIDED TISSUE SAMPLING IN PANCREATIC CANCER

CM Cho
1   Internal Medicine, School of Medicine, Kyungpook National University, Daegu, Korea, Republic of
,
SY Nam
1   Internal Medicine, School of Medicine, Kyungpook National University, Daegu, Korea, Republic of
,
GC Kim
2   Radiology, School of Medicine, Kyungpook National University, Daegu, Korea, Republic of
,
AN Seo
3   Pathology, School of Medicine, Kyungpook National University, Daegu, Korea, Republic of
,
HI Bae
3   Pathology, School of Medicine, Kyungpook National University, Daegu, Korea, Republic of
› Author Affiliations
Further Information

Publication History

Publication Date:
18 March 2019 (online)

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Aims:

Preclinical research is recently advanced for in vitro drug testing using cancer cell lines and xenograft tissues. However, obtaining tumor cells from patients with pancreatic cancer are limited. Recently, methods to culture and/or propagate tumor tissue using specimens obtained from endoscopic ultrasound-guided tissue sampling (EUS-TS) were introduced. The aim of our study was to evaluate the feasibility of a three-dimensional (3D) spheroid culture using EUS-TS in pancreatic cancer.

Methods:

Patients with locally advanced or distant metastasis were prospectively enrolled. After the acquisition of tissue specimen for pathologic diagnosis by EUS-TS, additional EUS-TS was performed for 3D spheroid culture. The acquired specimens were processed to cultivate in culture media and/or Matrigel and observed serially by phase contrast microscopy to evaluate the growth of tumor cells. After appropriate growth of tumor cells, the specimen was examined to compare the similarity of histology between human cancer tissue and 3D spheroid tumor.

Results:

A total of 21 patients with suspected pancreatic mass lesions were enrolled between June 2017 and March 2018. After excluding five patients, EUS-TS specimen for 3D culture was obtained from 16 patients, who were diagnosed as ductal adenocarcinoma. Formation and maintenance of 3D spheroid culture at 2nd week was successfully constructed in 13 cases (81.3%) and successful tumor growth with double size was established in 6 cases (37.5%). Establishment of spheroid culture for over 2nd passages was noted in 3 cases (18.8%). The histology of 3D culture specimen was similar to that of human tumor tissue.

Conclusions:

This model, which is successfully created by means of EUS-guided core biopsy at the time of initial tumor diagnosis, can be a promising method for construction of in vitro models in the process of drug development and testing for pancreatic cancer.