CC BY-NC-ND 4.0 · Laryngorhinootologie 2020; 99(S 02): S126
DOI: 10.1055/s-0040-1710907
Abstracts
Oncology

Adipose Tissue derived Stem Cells as potential Stem Cell Source for the Tumor Microenvironment of Head-and Neck Tumors – Characterization of Proliferation-, Invasion- and Migration in Co-Culture

Anna MARIA Stefanie Buchberger
1   Klinik und Poliklinik für Hals-Nasen-Ohrenheilkunde, Klinikum rechts der Isar, Technische Universität München, München
,
D Utz
2   Klinik für Radioonkologie, Universitätsklinikum Tübingen, Tübingen
,
A Dierks
1   Klinik und Poliklinik für Hals-Nasen-Ohrenheilkunde, Klinikum rechts der Isar, Technische Universität München, München
,
B Wollenberg
1   Klinik und Poliklinik für Hals-Nasen-Ohrenheilkunde, Klinikum rechts der Isar, Technische Universität München, München
,
M Siegl
1   Klinik und Poliklinik für Hals-Nasen-Ohrenheilkunde, Klinikum rechts der Isar, Technische Universität München, München
,
A Pickhard
1   Klinik und Poliklinik für Hals-Nasen-Ohrenheilkunde, Klinikum rechts der Isar, Technische Universität München, München
› Author Affiliations
› Further Information
Also available at
 

Introduction Up to date, origin and function of mesenchymal stem cells (MSC) in the tumor microenvironment (TME) are controversially discussed. Adipose tissue derived stem cells (ASC) from the peritumoral fat tissue could be a possible stem cell source. The objective of this work was the characterization of the interaction of ASCs with head-and-neck squamous cell carcinoma (HNSCC) cells.

Material and Method In co-culture experiments with HNSCC cell lines and peritumoral as well as non-tumor-associated primary ASC cell lines invasion-, migration-, and proliferation assays were carried out to investigate the interaction. With ELISA and PCR (IL-6, IL-20RA, IL-20RB, IL-22RA1, IL-24) analyzed possible mechanisms of mutual affectation. Additionally, the effect of a 2Gy radiation (RTx) as well as stimulation with IL-6 was tested.

Results Migration and invasion behavior of the ASCs was significantly reflationary through co-culture with HNSCCS. HNSCCs showed a comparably less invasive and migratory performance. After RTx of the tumor supernatants ASC invasiveness and migration decreased pronounced while RTx of the HNSCC cell lines party led to an increase in ASC invasion. HNSCCs showed a significantly increased expression level of IL-24 through co-culture while IL-20 RB expression was significantly reduced. ASCs showed high expression levels of IL 6 which were additionally increased through 2Gy RTx. Proliferation of HNSCC was inhibitable due to IL-6, the expression level of IL-20 RB increased.

Discussion An increase in invasiveness and migration of ASCs in co-culture with HNSCCS could point towards the peritumoral fat tissue as possible stem cell origin for TME associated MSCs. Understanding the role of ASCs in the TME could lead to new therapeutic options.

Poster-PDF A-1341.PDF



Publication History

Article published online:
10 June 2020

© 2020. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

© Georg Thieme Verlag KG
Stuttgart · New York