CC BY-NC-ND 4.0 · Laryngorhinootologie 2020; 99(S 02): S135-S136
DOI: 10.1055/s-0040-1710939
Abstracts
Oncology

Characterization of adenosine receptor A2A (ADORA2A) mediated effects on the immunogenic tumor microenvironment (TME) in a head and neck squamous cell carcinoma (HNSCC) mouse model

M Brand
1   Universitätsklinikum Ulm, HNO-Klinik, Kopf-Hals-Chirurgie, Ulm
,
J Krüger
1   Universitätsklinikum Ulm, HNO-Klinik, Kopf-Hals-Chirurgie, Ulm
,
J Greve
1   Universitätsklinikum Ulm, HNO-Klinik, Kopf-Hals-Chirurgie, Ulm
,
Marie Nicole Theodoraki
1   Universitätsklinikum Ulm, HNO-Klinik, Kopf-Hals-Chirurgie, Ulm
,
J Döscher
1   Universitätsklinikum Ulm, HNO-Klinik, Kopf-Hals-Chirurgie, Ulm
,
S Laban
1   Universitätsklinikum Ulm, HNO-Klinik, Kopf-Hals-Chirurgie, Ulm
,
PJ Schuler
1   Universitätsklinikum Ulm, HNO-Klinik, Kopf-Hals-Chirurgie, Ulm
,
TK Hoffmann
1   Universitätsklinikum Ulm, HNO-Klinik, Kopf-Hals-Chirurgie, Ulm
,
C Brunner
1   Universitätsklinikum Ulm, HNO-Klinik, Kopf-Hals-Chirurgie, Ulm
› Author Affiliations
 

Introduction The immunogenic tumor microenvironment (TME) is a main focus of cancer research. Many different immunosuppressive mechanisms have been identified. Over the last years it has become evident that the adenosine signalling pathway and its suppressive function on effector cells of the immune system influence tumor growth and therapeutic success.

Materials & Methods The cellular immune system of an immunocompetent HNSCC mouse model (C3H/HeN) was analysed by flow cytometry and immunohistochemistry. Inhibition of the adenosine signalling pathway was achieved by systemic blocking of the adenosine receptor A2A (ADORA2A).

Results Tumor formation leads to a complex immune response in immunocompetent mice. Beside significant alterations in the immunogenic microenvironment of the tumor, the immune cell populations obtained from blood, spleen and bone marrow showed significant changes over time. In mice, inhibition of ADORA2A on lymphocytes leads to a significant reduction in tumor mass and to an increased migration of regulatory T-cells and mature B-cells into the tumor tissue. In addition, germinal center-like-structures within the tumor tissue have been detected.

Conclusion We characterized a powerful tool to investigate the influence of different immune cell populations on tumor growth as well as for the investigation of new immunomodulatory cancer therapies. We showed a complex immunogenic reaction in different organs during tumor growth. By blocking the adenosine signaling pathway on lymphocytes, a drastic change in the immunogenic microenvironment of the tumor promotes antitumor function in HNSCC bearing C3H/HeN mice. Therefore, the adenosine signaling pathway represent a promising target for new therapeutic strategies for HNSCC treatment.

Poster-PDF A-1397.PDF



Publication History

Article published online:
10 June 2020

© 2020. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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