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CC BY-NC-ND 4.0 · Int Arch Otorhinolaryngol 2021; 25(03): e416-e420
DOI: 10.1055/s-0040-1715587
Original Research

High CD4+ T-Cell/B-Cell Ratio in the Paranasal Sinus Mucosa of Patients with Eosinophilic Chronic Rhinosinusitis

Akiko Inoue 
1   Department of Otorhinolaryngology, Toho University Omori Medical Center, Tokyo, Japan
2   Department of Molecular Immunology, Toho University School of Medicine, Tokyo, Japan
,
Yuriko Tanaka 
2   Department of Molecular Immunology, Toho University School of Medicine, Tokyo, Japan
,
Shinya Ohira
1   Department of Otorhinolaryngology, Toho University Omori Medical Center, Tokyo, Japan
,
Kentaro Matsuura
1   Department of Otorhinolaryngology, Toho University Omori Medical Center, Tokyo, Japan
,
Motonari Kondo 
2   Department of Molecular Immunology, Toho University School of Medicine, Tokyo, Japan
,
Kota Wada
1   Department of Otorhinolaryngology, Toho University Omori Medical Center, Tokyo, Japan
› Author Affiliations Funding Information JSPS KAKENHI (Grant/Award Number: 'JP17K11367' 'JP26462588'), Toho University School of Medicine Yanase Takeshi Scholarship Fund. The Research Promotion Grant from Toho University Graduate School of Medicine (Grant/Award Number: '19-02'), Toho University School of Medicine Project Research Funding (Grant/Award Number: '28-22'), Toho University Grant for Research Initiative Program (Grant/Award Number: 2020).
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Abstract

Introduction Chronic rhinosinusitis (CRS) is commonly classified based on the presence or absence of nasal polyps (NPs). Eosinophil infiltration is observed in NPs of patients in Western countries. In contrast, in East Asian countries, including Japan, CRS with NPs (CRSwNP) is subdivided based on the presence (eosinophilic CRS [ECRS]) or absence (non-eosinophilic CRS [NECRS]) of eosinophils in NPs. However, detailed analyses of other immune cells, such as lymphocytes, in NPs have not been performed. Therefore, clarification of the types of cells that infiltrate NPs is important to understand CRS pathogenesis.

Objectives We analyzed the lymphocytes that infiltrate the paranasal sinus mucosa of ECRS and NECRS patients.

Methods Eighteen patients with CRSwNP participated in this study, out of whom 6 were NECRS patients, and 12 were ECRS patients. The mucosa specimens, collected from patients during sinus surgeries, were subjected to collagenase treatment to prepare single cell suspensions. Then, mononuclear cells were isolated, and CD4+ T, CD8+ T, and CD20+ B-cell populations were examined using flow cytometry.

Results In both NECRS and ECRS patients, CD8+ T-cells were dominant over CD4+ T-cells. Notably, CD4+ T-cell/B-cell ratio, but not CD8+ T-cell/B-cell or CD4+ T-cell/CD8+ T-cell ratios, was significantly higher in ECRS patients than in NECRS patients.

Conclusion The CD4+ T-cell/B-cell ratio can be used as a potential indicator to differentiate between ECRS and NECRS.

Keywords

paranasal sinus disease - nasal mucosa - CD4-positive T-lymphocytes


Publication History

Received: 24 April 2020

Accepted: 05 July 2020

Article published online:
30 September 2020

© 2020. Fundação Otorrinolaringologia. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commecial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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