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Effect of Nosodes on Lettuce, Parasitized or Not by Meloidogyne enterolobiiFunding Partial funding support was obtained though the crowd-funding platform Catarse (https://www.catarse.me/). Catarse had no interference in any aspect of this work or its publication.
Background Nosodes are homeopathic preparations (HPs) obtained from tissues or substances associated with the targeted disease or from culture of the pathogenic agent. Nosodes are thought to modulate host resistance, easing symptoms or promoting cure. A few studies have been published about control of plant-parasitic nematodes with HPs, but none with nosodes. Conceptually, nosodes prepared from nematode infective stages might interact with the plant's pathogen-recognition system and initiate or modulate plant resistance to nematodes.
Objective Our goal was to investigate whether nosodes prepared from second-stage juveniles (J2) of Meloidogyne enterolobii can affect the moderate resistance already existing in the lettuce cultivar ‘Elisa’.
Methods Nosodes at the Hahnemannian concentrations (cH) 6, 18, 30 and 42 were applied on lettuce plants through irrigation, with a constant daily dosage. The nosode treatment started at the seedling stage, before nematode inoculation with 3,000 eggs + J2 per plant. A series of absolute and relative controls, and 10 replicates per treatment, were employed. At harvest, variables related to plant growth and nematode reproduction were assessed.
Results The nosode at 6, 18, and 30cH reduced (p <0.05) the nematode reproduction factor and root density. The nosode effect was cH-dependent since nematode reproduction was favored by treatment with 42cH. The nosode also affected (p <0.05) lettuce roots, which presented higher or lower fresh weight and volume depending on the cH applied and the condition—parasitized or not.
Conclusion Nosodes obtained from Meloidogyne J2 may affect plant parasitism by nematodes, possibly by interfering with plant resistance. The nature—positive or negative—and intensity of the nosode effect depends on the cH applied to the plants. Further studies are necessary to identify which cH values are more effective in reducing nematode reproduction without causing negative side effects on plant growth.
• Nosodes applied though irrigation can affect plant parasitism by nematodes.
• The nosode effect on nematode reproduction is cH-dependent.
• The nosode effect on plant growth is cH-dependent.
• The potential of nosodes to control plant-parasitic nematodes is worth further investigation.
Received: 17 November 2020
Accepted: 05 January 2021
Article published online:
04 May 2021
© 2021. Faculty of Homeopathy. This article is published by Thieme.
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- 1 Betti L, Trebbi G, Nani D. et al. Models with plants, microorganisms and viruses for basic research in homeopathy. In: Bonamin LV. ed. Signals and Images. Dordrecht: Springer; 2008: 97-111
- 2 Betti L, Trebbi G, Majewsky V. et al. Use of homeopathic preparations in phytopathological models and in field trials: a critical review. Homeopathy 2009; 98: 244-266
- 3 Carneiro SMTPG, Oliveira BG, Ferreira IF. Efeito de medicamentos homeopáticos, isoterápicos e substâncias em altas diluições em plantas: revisão bibliográfica. Rev Homeopatia 2011; 74: 9-32
- 4 Carneiro SMTPG, Teixeira MZ. Homeopatia e controle de doenças de plantas e seus patógenos. Sci Agrár Parana 2018; 17: 250-262
- 5 Sankar K, Jadhav AP. Nosodes and sarcodes. Indian J Tradit Knowl 2017; 16: 158-163
- 6 Andersen EJ, Ali S, Byamukama E, Yen Y, Nepal MP. Disease resistance mechanisms in plants. Genes (Basel) 2018; 9: 339
- 7 Carneiro SMTPG, Romano EDB, Pignoni E, Teixeira MZ, Vasconcelos MEC, Gomes JC. Effect of biotherapic of Alternaria solani on the early blight of tomato-plant and the in vitro development of the fungus. Int J High Dilution Res 2010; 9: 147-155
- 8 Rossi F, Melo PCT, Pascholatu SF. et al. Aplicação de bioterápicovisando induzir resistência em tomateiro contra mancha bacteriana. Rev Bras Agroecologia 2007; 2: 858-861
- 9 Shah-Rossi D, Heusser P, Baumgartner S. Homeopathic treatment of Arabidopsis thaliana plants infected with Pseudomonas syringae . ScientificWorldJournal 2009; 9: 320-330
- 10 Diniz LP, Maffia LA, Dhingra OD, Casali VWD, Santos RHS, Mizubuti ESG. Avaliação de produtos alternativos para controle da requeima do tomateiro. Fitopatol Bras 2006; 31: 171-179
- 11 Rauber LP, Boff MIC, Silva Z, Ferreira A, Boff P. Manejo de doenças e pragas da batateira pelo uso de preparados homeopáticos e variabilidade genética. Rev Bras Agroecologia 2007; 2: 1008-1011
- 12 Ferreira ICPV, Araújo AV, Gomes JG, Sales NLP. Preparados homeopáticos, extrato de bartimão e urina de vaca: alternativas para o controle da fusariose do abacaxi. Rev Bras Agroecologia 2009; 4: 2948-2951
- 13 Lorenzetti E, Standarlin JR, Treib EL. et al. Antimicrobial action against of Macrophomina phaseolina and control of the grey stem in soybean by homeopathic remedies nosode and sulphur. Afr J Agric Res 2016; 11: 3412-3427
- 14 Trebbi G, Nipoti P, Bregola V. et al. Ultrahigh diluted arsenic reduces spore germination of Alternaria brassicicola and dark leaf spot in cauliflower. Hortic Bras 2016; 34: 318-325
- 15 MacCarter J. Molecular approaches toward resistance to plant-parasitic nematodes. In: Howard Berg R, Taylor CG. eds. Cell Biology of Plant Nematode Parasitism. Plant Cell Monographs. Dorcrecht: Springer; 2008: 239-267
- 16 Datta SC. Effects of Cina on root-knot disease of mulberry. Homeopathy 2006; 95: 98-102
- 17 Sukul NC, Ghosh S, Sukul A, Sinhababu SP. Amelioration of root-knot disease of lady's finger plants by potentized Cina and Santonin. Homeopathy 2006; 95: 144-147
- 18 Swarowsky RA, Stangarlin JR, Kuhn OJ. et al. Influence of high dilutions of Cina for the control of Meloidogyne incognita in tomato plants. Am J Plant Sci 2014; 5: 3695-3701
- 19 Dias MH, Barbosa JA, Peters FF. et al. Controle alternativo de Meloidogyne incognita em tomateiro. Sci Agrár Parana 2016; 15: 421-426
- 20 Mioranza TM, Stangarlin JR, Müller MA. et al. Control of Meloidogyne incognita in tomato plants with highly diluted solutions of Thuya occidentalis and their effects on plant growth and defense metabolism. Semin Cienc Agrar 2017; 38: 2187-2220
- 21 Hanif A, Dawar S. Application of homeopathic medicines in addition with synthetic chemicals in the control of root rot and root knot pathogens and on the growth of crop plants. Int J Biol Biotechnol 2018; 15: 285-300
- 22 Hanif A, Dawar S. Effect of homeopathic nematicide pellets on plant-nematode interaction under controlled conditions. Pak J Bot 2019; 51: 367-375
- 23 Hanif A, Dawar S. Use of homeopathic pellets and globules diffusates against root rot and root knot pathogens on the growth of crop plants. In: Noor MA. ed. Research and Development in Agricultural Sciences, vol. 1. Hooghly: Book Publisher International; 2020: 1-46
- 24 Ferreira TM, Mangeiro MZ, Almeida AM, Souza RM. Effect of homeopathic preparations on lettuce, parasitized or not by Meloidogyne enterolobii . Homeopathy 2021; 110: 115-121
- 25 Jones J, Gheysen G, Fenoll C. Genomics and Molecular Genetics of Plant-Nematode Interactions. Dordrecht: Springer; 2011
- 26 Holbein J, Grundler FMW, Siddique S. Plant basal resistance to nematodes: an update. J Ex Bot 2016; 3: 2049-2061
- 27 Coolen WA, D'Herde CJ. A Method for the Quantitative Extraction of Nematodes from Plant Tissue. Ghent: State Nematology and Entomology Research Station; 1972
- 28 Agência Nacional de Vigilância Sanitária. Farmacopéia Homeopática Brasileira. 3rd ed.. Brasília: 2011. . Accessed November 15, 2020 at: http://portal.anvisa.gov.br/documents/33832/259147/3a_edicao.pdf/cb9d5888-6b7c-447b-be3c-af51aaae7ea8
- 29 Cotter HVT, Hicks CB, Simmons JA. Multiple egg harvests from Meloidogyne-infested tomato root systems. J Nematol 2003; 35: 331
- 30 Miranda GB, Souza RM, Viana AP. Assessment of methods and criteria for screening Psidium spp. for resistance to Meloidogyne enterolobii . Nematol Bras 2010; 34: 211-219
- 31 O'Neill ME, Mathews K. A weighted least squares approach to Levene's test of homogeneity of variance. Aust N Z J Stat 2000; 42: 81-100
- 32 Goverse A, Smant G. The activation and suppression of plant innate immunity by parasitic nematodes. Annu Rev Phytopathol 2014; 52: 243-265
- 33 Fenoll C, Grundler FMW, Ohl SA. Cellular and Molecular Aspects of Plant-Nematode Interactions. Dordrecht: Springer; 2012
- 34 Shukla N, Kaur P, Kumar A. Molecular aspects of plant-nematode interactions. Indian J Plant Physiol 2016; 21: 477-488
- 35 Majewsky V, Arlt S, Shah D. et al. Use of homeopathic preparations in experimental studies with healthy plants. Homeopathy 2009; 98: 228-243
- 36 Baumgartner S. The state of basic research on homeopathy. In: Witt C, Albrecht H. eds. New Directions in Homeopathy Research—Advice from an Interdisciplinary Conference. KVC-Verlag; 2009: 107-130
- 37 Baumgartner S. Status of basic research in homeopathy. In: von Ammon K, Baumgartner S, Behnke J. et al, eds. The Current State of Homeopathic Research. Kothen: Scientific Society for Homeopathy; 2016: 41-47