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CC BY 4.0 · Rev Bras Ginecol Obstet 2022; 44(11): 1047-1051
DOI: 10.1055/s-0042-1759630
Original Article
Assisted Fertilization

Comparison of the Effects of GMCSF-Containing and Traditional Culture Media on Embryo Development and Pregnancy Success Rates

Fatih Adanacıoglu 
1   Turan Cetin Private IVF Center, Adana, Turkey
,
Çağlar Çetin 
2   Department of Obstetrics and Gynecology, Bezmialem Vakif University School of Medicine, Istanbul, Turkey
,
Gozde Tokat 
3   Department of Embryology, Turan Cetin Private IVF Center, Adana, Turkey
,
Durdane Adanacıoglu 
4   Adana State Hospital, Adana, Turkey
,
Ayse Filiz Gokmen Karasu 
2   Department of Obstetrics and Gynecology, Bezmialem Vakif University School of Medicine, Istanbul, Turkey
,
Mehmet Turan Çetin 
1   Turan Cetin Private IVF Center, Adana, Turkey
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Abstract

Objective The use of granulocyte macrophage colony-stimulating factor (GM-CSF)-containing medium, which is a commercial medium that is used for cultivation of embryos in in vitro fertilization (IVF) treatments, has been suggested to increase the efficiency of this procedure in patients with previous multiple unsuccessful attempts. In this retrospective study, we analyzed GM-CSF-containing embryo culture media compared with traditional culture media in terms of development of embryos, pregnancy, and ongoing pregnancy success and live birth rates.

Methods This is a prospective case control study conducted in a single center. A total of 131 unexplained infertility patients were included in the study. A cohort of 69 patients whose embryos were cultured in GM-CSF-containing medium and a control group of 62 age-matched patients whose embryos were cultured in conventional Sage One Step medium were included in the study. The major study outcomes were achievement of pregnancy and ongoing pregnancy rate at 12 weeks of gestation.

Results The pregnancy and ongoing pregnancy rates of the patients whose embryos were cultured in GM-CSF-containing medium were 39.13% and 36.23%, respectively. These were higher than the rates of the control group, which were 30.65% and 29.03%, respectively, although this difference was not statistically significant. In addition, the 5th-day embryo transfer percentage in the GM-CSF group was higher than in the control group (34.78% versus 27.4%).

Conclusion The main findings of our study were that there was no difference between the GM-CSF-enhanced medium and the control group in terms of our major study outcomes. However, blastomere inequality rate and embryo fragmentation rates were lower in the GM-CSF group.

Keywords

in vitro fertilization - embryo culture - embryo transfer - granulocyte macrophage colony-stimulating factor

Contributions

All authors participated in the concept and design of the present study; analysis and interpretation of data; draft or revision of the manuscript, and they have approved the manuscript as submitted. All authors are responsible for the reported research.




Publikationsverlauf

Eingereicht: 02. Januar 2022

Angenommen: 09. September 2022

Artikel online veröffentlicht:
29. Dezember 2022

© 2022. Federação Brasileira de Ginecologia e Obstetrícia. This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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