Effect of Traditional Treatment of Diabetes Mellitus with Xanthosoma sagittifolium on the Male Reproductive System of Alloxan-Induced Diabetic Wistar Rats

Abstract

Introduction

The management of diabetes mellitus (DM) targets glycemic control as well as prevention or reversal of other complications associated with the metabolic disorder. One of such complications is the infertility which has been traced to oxidative stress and DNA damage caused by DM.

Subjects and methods

In this study, experimentally-induced diabetic male Wistar rats were fed with Xanthosoma sagittifolium (cocoyam) for 14 days and its effect on sperm morphology and characteristics, as well as histology of the testes were assessed. X. sagittifolium corm feed (Xs) were reconstituted with commercial rat feed to contain 25, 50, 75 or 100% X. sagittifolium on a weight/weight basis.

Result

One primary and 7 secondary cell abnormalities were observed with overall increased total abnormal sperm cells in the diabetic rats. Sperm cell motility was significantly (p<0.05) reduced in all diabetic rats, but diabetic rats fed with Xs showed increased motility and liveability. Sperm volume was unchanged, but the depressed sperm count in the untreated diabetic rats was increased in Xs-fed rats. Histology of Xs-fed diabetic rats showed reduction in seminiferous tubule diameter and luminal diameter which was significantly enlarged in untreated diabetic rats.

Conclusion

X. sagittifolium significantly (p<0.05) prevented further deterioration of sperm cell quality and reversed cell apoptosis as presented by increased sperm motility, liveability and count. In the overall, the chances of fertilization by sperm cells from diabetic patients will be increased as well as achievement of good glycemic control and prevention of further damage by the disease condition.

Key words

Xanthosoma sagittifolium - diabetes mellitus - andrology

• References

• 1 Halim EM. Lowering of blood sugar by water extract of Azadirachta indica and Abroma augusta in diabetes rats. Indian J Exp Biol 2003; 41: 636-640
• 2 Heinrich M, Pieroni A, Bremner P. Plants as medicines. In: Prance G, Nesbitt M. (ed.) The cultural history of plants. Routledge; 2005: 205-238 ISBN 0-415-92746-3
• 3 Pandey A, Tripathi P, Pandey R. et al. Alternative therapies useful in the management of diabetes: A systematic review. J Pharm Bioallied Sci 2011; 3: 504-512
• 4 Kaur J. A comprehensive review on metabolic syndrome. Cardiology Research and Practice 2014; 1–22: 943162
• 5 Veghari G, Sedaghat M, Joshaghani H. et al. Association between socio-demographic factors and diabetes mellitus in the north of Iran: A population-based study. Int J Diab Mellitus 2010; 2: 154-157
• 6 McDonald PAJ, Montenegro GJA, Cruz GCE. et al. Prevalence, sociodemographic distribution, treatment and control of diabetes mellitus in Panama. Diabetology & Metabolic Syndrome 2013; 5: 69-77
• 7 Wong ND. Intensified screening and treatment of the metabolic syndrome for cardiovascular risk reduction. Preventive Cardiology 2005; 8: 47-54
• 8 Mallidis C, Agbaje I, McClure N. et al. The influence of diabetes mellitus on male reproductive function: a poorly investigated aspect of male infertility. Urologe A 2011; 50: 33-37
• 9 Loeken MR. A new role for pancreatic insulin in the male reproductive axis. Diabetes 2012; 61: 1667-1668
• 10 Schoeller EL, Albanna G, Frolova AI. et al. Insulin rescues impaired spermatogenesis via the hypothalamic-pituitary-gonadal axis in akita diabetic mice and restores male fertility. Diabetes 2012; 61: 1869-1878
• 11 Agbaje IM, Rogers DA, McVicar CM. et al. Insulin dependant diabetes mellitus: Implications for male reproductive function. Hum Reprod 2007; 22: 1871-1877
• 12 Jangir RN, Jain GC. Diabetes mellitus induced impairment of male reproductive functions: a review. Curr Diabetes Rev 2014; 10: 147-157
• 13 Agbaje IM, McVicar CM, Schock BC. et al. Increased concentrations of the oxidative DNA adduct 7,8-dihydro-8-oxo-2-deoxyguanosine in the germ-line of men with type 1 diabetes. Reprod Biomed Online 2008; 16: 401-409
• 14 Mulholland J, Mallidis C, Agbaje I. et al. Male diabetes mellitus and assisted reproduction treatment outcome. Reprod Biomed Online 2011; 22: 215-219
• 15 Manyam BY. Diabetes mellitus, Ayurveda, and yoga. Comment. J Altern Complement Med. 2004; 10: 223-225
• 16 Dham S, Shah V, Hirsch S. et al. The role of complementary and alternative medicine in diabetes. Curr Diab Rep 2006; 6: 251-258
• 17 Acharya D, Anshu S. Indigenous herbal medicines: Tribal formulations and traditional herbal practices. Jaipur, India: Aavishkar publishers distributor; 2008. ISBN 978-81-7910-252-7 pp 440
• 18 Artus M, Croft P, Lewis M. The use of CAM and conventional treatments among primary care consulters with chronic musculoskeletal pain. BMC Family Practice 2007; 8: 26-40
• 19 Bloom BS, Retbi A, Dahan S. et al. Evaluation of randomized controlled trials on complementary and alternative medicine. International Journal of Technology Assessment in Health Care 2000; 16: 13-21
• 20 Oyeyemi MO, Ubiogoro O. Spermiogram and morphological characteristics in testicular and epididymal spermatozoa of large white boar in Nigeria. Int J Morphol 2005; 23: 235-239
• 21 Ramalho-Santos J, Amaral S, Oliveira PJ. Diabetes and the impairment of reproductive function: Possible role of mitochondria and reactive oxygen species. Current Diabetes Reviews 2008; 4: 46-54 (9)
• 22 Agarwal A, Said TM. Oxidative stress, DNA damage and apoptosis in male infertility: a clinical approach. BJU International 2005; 95: 503-507
• 23 Aitken RJ, De Iuliis GN, McLachlan RI. Biological and clinical significance of DNA damage in the male germ line. International Journal of Andrology 2008; 32: 46-56
• 24 Adeniji DA, Oyeyemi MO, Olugbenga JB. Sperm morphological characteristics and mating behavior of proviron treated west african dwarf bucks with testicular degeration. Int J Morph 2010; 28: 623-626
• 25 Ajala OO, Oyeyemi MO, Akusu MO. et al. The effects of scrotal insulation on the testicles and spermatozoa characteristics of west African dwarf goats. Sokoto J of Veterinary Sciences 2001; 3: 44-50
• 26 Oyeyipo IP, Raji Y, Emikpe BO. et al. Effects of nicotine on sperm characteristics and fertility profile in adult male rats: A possible role of cessation. J Reprod Infertil 2011; 12: 201-207
• 27 Yoshida M, Kawano N, Yoshida K. Control of sperm motility and fertility: diverse factors and common mechanisms. Cell Mol Life Sci 2008; 65: 3446-3457