Drug Res (Stuttg) 2017; 67(09): 527-533
DOI: 10.1055/s-0043-109001
Original Article
© Georg Thieme Verlag KG Stuttgart · New York

Evaluation of Bone Mineral Density in Children with Type I Diabetes Mellitus and Relationship to Serum Levels of Osteopontin

Forough Saki1, *, Abdolkarim Sheikhi2, *, Gholam Hosein Ranjbar Omrani1, Hamid Karimi1, Mohammad Hossein Dabbaghmanesh1, Seyed Nouraddin Mousavinasab3
  • 1Shiraz Endocrinology and Metabolism Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
  • 2Department of Immunology, Faculty of Medicine, Dezful University of Medical Sciences, Dezful, Iran
  • 3Department of Biostatistics, Faculty of Health, Mazandaran University of Medical Sciences, Sari, Iran
Further Information

Publication History

received 27 November 2016

accepted 11 April 2017

Publication Date:
12 May 2017 (eFirst)



Type I diabetes mellitus (T1DM) patients are at risk of osteoporosis and fracture due to the osteoblast and osteoclast malfunction. Osteopontin (OPN) as the major non-collagenous bone matrix protein is produced by osteoblasts and osteoclasts and involve in bone resorption, formation and remodeling. To evaluate the serum levels of OPN, bone mineral density (BMD) and correlation in patients with T1DM this study was designed.


In this case-control study, 87 children with T1DM and 87 age/sex-matched healthy subjects were enrolled. Blood samples were tested for OPN levels by ELISA. Dual energy X-ray absorptiometry was used to measure BMD.


The mean levels of BMD in patients was significantly lower than control group (p<0.0001). There was no significant difference between patients and healthy subjects regarding the levels of OPN. However, in patients with high levels of OPN (mean+1.5 standard deviation) the BMD was significantly lower than other patients (p<0.0001). Totally there was a negative correlation between serum levels of OPN and BMD in patients with T1DM (p<0.016).


These results indicated that the BMD in T1DM is less than healthy children and elevated level of OPN in patients could be associated with low BMD. A linear negative correlation between serum OPN and total BMD of T1DM patients compared to control group was noticed in this study indicating that the amount of serum OPN could be effective on BMD and a good predicting factor for osteoporosis in patients.

* The first and second authors are of equal rights.