Endoscopy 2024; 56(S 02): S95
DOI: 10.1055/s-0044-1782895
Abstracts | ESGE Days 2024
Oral presentation
Future perspectives in imaging and tissue acquisition for pancreatic lesions 26/04/2024, 15:30 – 16:30 Room 8

Feasibility of endoscopic ultrasound-guided fine needle biopsy for generating patient-derived organoids of pancreatic ductal adenocarcinoma: preliminary results from a single-center prospective database

F. Borrelli de Andreis
1   Digestive Endoscopy Unit, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, Rome, Italy
2   Digestive Endoscopy Unit, Fatebenefratelli Gemelli Isola Tiberina, Rome, Italy
,
V. Panzeri
3   Department of Neuroscience, Section of Human Anatomy, Catholic University of the Sacred Heart, Rome, Italy
,
V. Ruta
3   Department of Neuroscience, Section of Human Anatomy, Catholic University of the Sacred Heart, Rome, Italy
,
E. Cesari
4   GSTeP Organoids Research Core Facility, Fondazione Policlinico A. Gemelli IRCCS, Rome, Italy
,
F. Attili
1   Digestive Endoscopy Unit, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, Rome, Italy
,
C. Spada
1   Digestive Endoscopy Unit, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, Rome, Italy
,
C. Sette
3   Department of Neuroscience, Section of Human Anatomy, Catholic University of the Sacred Heart, Rome, Italy
› Institutsangaben
› Weitere Informationen
Auch verfügbar aufeRef
 

Aims Pancreatic ductal adenocarcinoma (PDAC) is notorious for its aggressive nature and grim prognosis, underscoring the urgent need for personalized pre-clinical models to develop targeted therapies. Patient-derived organoids (PDOs) are three-dimensional models of human tumors that have emerged as promising research tools. We therefore aimed to assess the feasibility of endoscopic ultrasound-guided fine needle biopsy (EUS-FNB) for generating PDAC-PDOs.

Methods A single-center prospective database was established to collect data from suspected PDAC patients undergoing EUS-FNB for histological confirmation between May and October 2023. All biopsies were performed using a 22G-needle. Two to three biopsies were taken for histological examination before performing a single-pass FNB to establish PDO cultures. The primary outcome was the successful propagation of PDAC-PDOs for at least three passages (P3). Secondary outcomes included sample adequacy, diagnostic rate, and safety.

Results A total of 22 patients (M:F 13:9, median age 66.5 years, range 55-86) were enrolled. One case was subsequently diagnosed as pancreatic lymphoma and excluded. PDAC-PDOs were successfully established in 9 out of 21 PDAC cases at P3, resulting in a success rate of 43%. The median size of the 9 PDAC from which PDOs were generated was 4 cm (range 1.2-5). 5 masses were located in the pancreatic head, 1 in the neck, 1 in the body, and 2 in the tail. Sample adequacy and diagnostic rates were both achieved in 100% of subjects. The presence of a biliary stent during EUS-FNB did not impact the success rate of PDAC-PDO generation (n=1 in the generated PDAC-PDOs group vs n=3 in the non-generated PDAC-PDOs group; p=0.254). No adverse events related to the procedure occurred. [1]

Conclusions This study demonstrates that PDAC-PDOs can be successfully generated through EUS-FNB sampling with a single-pass fine needle biopsy, without compromising the safety profile of the procedure.



Publikationsverlauf

Artikel online veröffentlicht:
15. April 2024

© 2024. European Society of Gastrointestinal Endoscopy. All rights reserved.

Georg Thieme Verlag KG
Rüdigerstraße 14, 70469 Stuttgart, Germany

 

  • References

  • 1 Chen S, Wang M, Liu L. et al. Ultrasound-guided fine-needle aspiration/biopsy-based pancreatic organoids establishment: an alternative model for basic and preclinical research. Gastroenterology Report Volume 11 2023
    PubMed