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CC BY-NC-ND 4.0 · Journal of Gastrointestinal Infections
DOI: 10.1055/s-0045-1812477
Original Article

Utility of the NEUT-RI and NEUT-GI Parameters of Sysmex XN 2000 in Acute Intestinal Bacterial Infections

Autoren

  • Luciana Biscozzo

    1   Department of Clinical Pathology and CERICSAL (CEntro di RIcerca Clinico SALentino), “Veris delli Ponti Hospital,” Scorrano, Lecce, Italy

  • Maria Cazzato

    2   Department of Gastroenterology and CERICSAL (CEntro di RIcerca Clinico SALentino), “Veris delli Ponti Hospital,” Scorrano, Lecce, Italy

  • Mauro Serra

    2   Department of Gastroenterology and CERICSAL (CEntro di RIcerca Clinico SALentino), “Veris delli Ponti Hospital,” Scorrano, Lecce, Italy

  • Primaldo Paiano

    2   Department of Gastroenterology and CERICSAL (CEntro di RIcerca Clinico SALentino), “Veris delli Ponti Hospital,” Scorrano, Lecce, Italy

  • Mario Laneve

    1   Department of Clinical Pathology and CERICSAL (CEntro di RIcerca Clinico SALentino), “Veris delli Ponti Hospital,” Scorrano, Lecce, Italy

  • Francesca De Giorgi

    3   CERICSAL (CEntro di RIcerca Clinico SALentino), “Veris delli Ponti Hospital,” Scorrano, Lecce, Italy

  • Alberto Argentiero

    3   CERICSAL (CEntro di RIcerca Clinico SALentino), “Veris delli Ponti Hospital,” Scorrano, Lecce, Italy

  • Giovanni Pecorella

    4   Department of Obstetrics and Gynecology, and CERICSAL (CEntro di RIcerca Clinico SALentino), “Veris delli Ponti Hospital,” Scorrano, Lecce, Italy

  • Martina Licchelli

    4   Department of Obstetrics and Gynecology, and CERICSAL (CEntro di RIcerca Clinico SALentino), “Veris delli Ponti Hospital,” Scorrano, Lecce, Italy

  • Andrea Tinelli‡   *

    4   Department of Obstetrics and Gynecology, and CERICSAL (CEntro di RIcerca Clinico SALentino), “Veris delli Ponti Hospital,” Scorrano, Lecce, Italy

  • Luigi Tagliaferro  ‡   *

    1   Department of Clinical Pathology and CERICSAL (CEntro di RIcerca Clinico SALentino), “Veris delli Ponti Hospital,” Scorrano, Lecce, Italy

Funding None.
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Abstract

Background

Bacterial infections constitute a predominant factor contributing to morbidity and mortality; effective management of patients necessitates an accurate and timely diagnosis.

Objectives

To evaluate, in the early diagnosis of intestinal bacterial infection, the quantitative variations in the values of two parameters related to the functional state and the performance of neutrophils, called NEUT-RI (neutrophil-reactivity intensity) and NEUT-GI (neutrophil-granularity intensity), correlating them with white blood cells (WBCs), erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), and procalcitonin (PCT).

Methods

A total of 57 intestinal bacterial infection–positive patients were retrospectively compared with 65 healthy subjects (control group). The identification of pathogens in fecal material samples was conducted through a molecular method, real-time multiplex PCR (FilmArray GI Panel BioFire, Biomerieux). WBC, NEUT-RI, and NEUT-GI were analyzed using the Sysmex XN 2000 hematology analyzer (Sysmex, Kobe, Japan). The Test-1 Alifax instrument was used to determine the ESR, while CRP and PCT were determined on the Beckman DxC AU 700 and Biomerieux VIDAS 3 instruments. Statistical evaluation was conducted employing Jamovi software.

Results

NEUT-RI and NEUT-GI were higher in subjects with intestinal bacterial infection, compared with healthy subjects: p < 0.001 and p = 0.002, respectively; in distinguishing subjects with infection from healthy subjects, NEUT-RI and NEUT-GI achieved both significant but moderate accuracy, with receiver operating characteristic area under the curve of 0.788 for NEUT-RI and 0.669 for NEUT-GI.

Conclusion

The parameters NEUT-RI and NEUT-GI allow us to measure the activation state of neutrophils, which are key actors in the innate immune response. Based on the case series examined, they appear to support the diagnosis of bacterial intestinal infection with moderate efficacy.

Keywords

intestinal bacterial infections - neutrophil-granularity intensity (NEUT-GI) - neutrophil-reactivity intensity (NEUT-RI)

Authors Contributions

L.B., L.T., and A.T. contributed to the design of the study and data analysis. L.B., M.C., M.S., and P.P. were responsible for data collection, while M. Licchelli and M. Laneve developed the methodology. G.P. and A.A. handled data curation, and A.A. together with F.D.G. managed the ethical committee documentation. Manuscript writing was carried out by L.B. and F.D.G., with revisions by A.T. and L.T. Study supervision was provided by A.T. and L.T.


Data Availability Statement

Data sharing is not applicable to this article as no new data were created or analyzed in this study.


Ethical Approval Statement

The study protocol was approved by the Local Ethics Committee (prot. 1840/CEL).


Consent to Participate

Not necessary, as this is a retrospective observational study.


* These authors contributed equally to this work.




Publikationsverlauf

Eingereicht: 29. Januar 2025

Angenommen: 30. Juli 2025

Artikel online veröffentlicht:
09. Oktober 2025

© 2025. Gastrointestinal Infection Society of India. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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