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Plant Biol (Stuttg) 2001; 3(4): 357-363
DOI: 10.1055/s-2001-16459
Original Paper
Georg Thieme Verlag Stuttgart ·New York

An Auxin Transport Inhibitor Interferes With Unicellular Gravitropism in Protonemata of the Moss Ceratodon purpureus

J. Schwuchow, W. Michalke, R. Hertel
  • Institut für Biologie III der Albert-Ludwigs-Universität Freiburg, Freiburg, Germany
Further Information

Publication History

January 2, 2001

April 23, 2001

Publication Date:
16 August 2001 (online)

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Abstract

Gravitropism of the protonemata of the moss Ceratodon purpureus (Hedw.) Brid. was studied after treatment with auxin transport inhibitors and auxin-related substances. The phytotropins NPA (naphthylphthalamic acid) and PBA (pyrenoylbenzoic acid), known to block auxin efflux in higher plants, strongly inhibited gravitropic curvature of the apical protonemal cell. At 3 μM NPA or PBA, approximately 60 % inhibition of curvature was observed; growth rates were less affected. Tyrphostin A47, a known antagonist of NPA effects in higher plants, released the inhibition of moss protonemal gravitropism and restored the full curvature response. Exogenous IAA, even at high concentrations (40 μM), did not interfere with protonemal gravitropism. To account for the results, modified hypotheses for auxin transport and action are discussed.

Abbreviations

IAA: indole-3-acetic acid

1-NAA: 1-naphthaleneacetic acid

NPA: N-1-naphthylphthalamic acid

PBA: 2-(1-pyrenoyl)benzoic acid

TIBA: 2,3,5-triiodobenzoic acid

tyrphostin A47: 3,4-dihydroxybenzylidene-thiocyanoacetamide

Key words

Auxin transport - Ceratodon - gravitropism - moss - NPA - tyrphostin

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R. Hertel

Institut Biologie III Universität

Schänzlestr. 1
79104 Freiburg
Germany

Email: hertel@uni-freiburg.de

Section Editor: M. Koornneef

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