Background and Study Aims: The present authors have already reported that mucosal autofluorescence intensity
was increased in gastric lesions induced by nonsteroidal anti-inflammatory drugs.
The aim of this study was to clarify whether the observation of mucosal autofluorescence
with a newly established endoscopic fluorescence analyzing system could help us to
recognize indomethacin-induced gastric lesion formation in vivo.
Materials and Methods: Gastric mucosal fluorescence intensity was measured time-sequentially after indomethacin
treatment in rats, using a fluorescence endoscope system. The concentration of thiobarbituric
acid-reactive substances in gastric mucosa was measured as an indicator of tissue
lipid peroxidation. Fluorescent substances from rat stomachs were analyzed using high
performance liquid chromatography.
Results: Treatment with indomethacin induced a time-dependent increase of fluorescence intensity.
The concentration of thiobarbituric acid-reactive substances was also increased after
the treatment. Pretreatment with radical scavenging reagent constrained the increase
of both the fluorescence intensity and the concentration of thiobarbituric acid-reactive
substances. The fluorescence products were coproporphyrin, protoporphyrin and mesoporphyrin.
Conclusions: These findings indicate that the intensity of porphyrin fluorescence increased in
gastric mucosal lesions induced by oxygen radicals. Endoscopic observation of mucosal
fluorescence was shown to aid the sensitive and objective diagnosis of gastric injuries.
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H. Matsui, M. D.
Division of Gastroenterology · Institute of Clinical Medicine · University of Tsukuba
1-1-1 Ten-nohdai, Tsukuba · Ibaraki 305-8575 · Japan
Fax: + 81-298-527408 ·
eMail: hmatsui@md.tsukuba.ac.jp
