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Semin Reprod Med 2002; 20(1): 045-050
DOI: 10.1055/s-2002-23519
Copyright © 2002 by Thieme Medical Publishers, Inc., 333 Seventh Avenue, New York, NY 10001, USA. Tel.: +1(212) 584-4662

Cryopreservation of Immature and Mature Human Oocytes

J. D. Wininger, H. I. Kort
  • Reproductive Biology Associates, Atlanta, Georgia
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Publication History

Publication Date:
02 April 2002 (online)

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ABSTRACT

Cryopreservation of oocytes facilitates the long-term storage of oocytes for patients in danger of losing ovarian function. It also alleviates many of the ethical concerns associated with embryo cryopreservation. Problems associated with metaphase II oocyte cryopreservation include zona pellucida hardening and spindle damage. The cryopreservation of germinal vesicle-stage oocytes has been undertaken as a means of circumventing the problem of spindle damage in mature oocytes. One of the main disadvantages of immature oocyte cryopreservation is the fact that in vitro maturation is required post-thaw. The majority of live births from oocyte cryopreservation have involved the use of 1,2-propanediol and slow freezing protocols. Various methods have been used in an attempt to improve survival rates. These include vitrification and use of novel cryopreservatives. Future areas of concentration should include in vitro maturation, vitrification, and alternate cryopreservatives.

KEYWORD

Cryopreservation - oocyte - metaphase II - germinal vesicle

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