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Plant Biol (Stuttg) 2002; 4(5): 619-624
DOI: 10.1055/s-2002-35436
Original Paper
Georg Thieme Verlag Stuttgart ·New York

Interaction between Cytokinesis-Related Callose and Cortical Microtubules in Dividing Cells of the Liverwort Riella helicophylla

P. Scherp 1 , R. Grotha 2 , U. Kutschera 2
  • 1 Biology Department, University of Louisiana, Lafayette, USA
  • 2 FB 19 Pflanzenphysiologie, Universität Kassel, Kassel, Germany
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Publikationsverlauf

Received: April 11, 2002; Revision of July 16, 2002

Accepted: September 10, 2002

Publikationsdatum:
15. November 2002 (online)

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Abstract

In juvenile walls of dividing cells of the liverwort Riella helicophylla the nitroso-derivative of photolysed Nifedipine (a calcium antagonist) stimulates the deposition of callose. This enhanced biosynthesis of β-1,3-glucan can only be observed in the cell plate, the juvenile cell walls and the walls of adjacent cells. An immunocytological analysis of this effect revealed that no cortical microtubules occurred at the sites of callose deposition. The cells of the control displayed a normal distribution of cortical microtubules at the plasma membrane as long as no callose was deposited along the corresponding walls. In a second set of experiments, inhibitors of microtubule polymerization and depolymerization (amiprophosmethyl and taxol, respectively) were used. At low concentrations, these substances also caused a significant stimulation of callose deposition in the plane of cell division. Based on these findings, we propose a regulatory model of callose and cellulose biosynthesis that depends on the binding of the cellulose/callose synthase complex to cortical microtubules that may be mediated by unknown binding protein(s).

Abbreviations

ND-NIF: nitroso-derivative of photolysed Nifedipine

APM: amiprophosmethyl

Key words

Callose deposition - cortical microtubules - Riella

References

  • 01 Baskin,  T. I.. (2001);  On the alignment of cellulose microfibrils by cortical microtubules: a review and a model.  Protoplasma. 215 150-171
    Suche in Google Scholar
  • 02 Granger,  C. L., and Cyr,  R. J.. (2000);  Microtubule reorganization in tobacco BY-2 cells stably expressing GFP-MBD.  Planta. 210 502-509
    Suche in Google Scholar
  • 03 Grotha,  R.. (1986);  Tetracyclines, verapamil and nifedipine induce callose deposition at specific sites in Riella helicophylla. .  Planta. 169 546-554
    Suche in Google Scholar
  • 04 Hong,  Z.,, Delauney,  A. J.,, and Verma,  D. P. S.. (2001 a);  A cell plate specific callose synthase and its interaction with phragmoplastin.  Plant Cell. 13 755-768
    Suche in Google Scholar
  • 05 Hong,  Z.,, Zhang,  Z.,, Olson,  J. M.,, and Verma,  D. P. S.. (2001 b);  A novel UDP-glucose transferase is part of the callose synthase complex and interacts with phragmoplastin at the forming cell plate.  Plant Cell. 13 769-779
    Suche in Google Scholar
  • 06 Howe,  M. A., and Underwood,  L. M.. (1903);  The genus Riella with descriptions of new species from North America and the Canary Islands.  Bull. Torr. Bot. Club. 30 214-224
    Suche in Google Scholar
  • 07 Kauss,  H.. (1987);  Some aspects of calcium-dependent regulation in plant metabolism.  Annu. Rev. Plant Physiol.. 38 47-72
    Suche in Google Scholar
  • 08 Kumagai,  F., and Hasezawa,  S.. (2001);  Dynamic organization of microtubules and microfilaments during cell cycle progression in higher plant cells.  Plant Biol.. 3 4-16
    Suche in Google Scholar
  • 09 Kutschera,  U., and Heiderich,  A.. (2002);  Sucrose metabolism and cellulose biosynthesis in sunflower hypocotyls.  Physiol. Plant.. 114 372-379
    Suche in Google Scholar
  • 10 Samuels,  A. L.,, Giddings,  T. H. jr.,, and Staehelin,  L. A.. (1995);  Cytokinesis in tobacco BY-2 and root tip cells: a new model of cell plate formation in higher plants.  J. Cell Biol.. 13 1345-1357
    PubMedSuche in Google Scholar
  • 11 Scherp,  P.. (2001) Untersuchungen zum Vorkommen von Callose im Pflanzenreich und zur Regulation der Biosynthese des β-1,3-Glucans in Zellplatten und jungen Zellwänden bei Riella helicophylla und Arabidopsis thaliana unter Berücksichtigung der Wirkung von Nifedipin.  Universität Kassel; Dissertation
    Suche in Google Scholar
  • 12 Scherp,  P.,, Grotha,  R.,, and Kutschera,  U.. (2001 a);  Occurrence and phylogenetic significance of cytokinesis-related callose in green algae, bryophytes, ferns and seed plants.  Plant Cell Rep.. 20 143-149
    CrossrefPubMedSuche in Google Scholar
  • 13 Scherp,  P.,, Grotha,  R.,, and Kutschera,  U.. (2001 b);  Deposition of cytokinesis-related callose in Riella helicophylla and Arabidopsis thaliana: Effects of photolytically altered Nifedipine.  Plant Biol.. 3 311-318
    Thieme ConnectPubMedSuche in Google Scholar
  • 14 Stange,  L.. (1984);  Meristem organization and morphogenesis in Gemmae of Riella helicophylla (Bory et. Mont.) Mont..  J. Hattori Bot. Lab.. 56 195-200
    PubMedSuche in Google Scholar
  • 15 Stange,  L.. (1992);  Self-organized regulation of cell growth in Riella helicophylla (Bory et Mont.) Mont.  Crypt. Bot.. 3 40-44
    PubMedSuche in Google Scholar
  • 16 Studhalter,  R. A., and Cox,  M. E.. (1941);  The gemmaling of Riella americana. .  The Bryologist. 44 77-93
    PubMedSuche in Google Scholar
  • 17 Verma,  D. P. S.. (2001);  Cytokinesis and building of the cell plate in plants.  Annu. Rev. Plant Physiol. Plant Mol. Biol.. 52 751-784
    CrossrefPubMedSuche in Google Scholar
  • 18 Verma,  D. P. S., and Hong,  Z.. (2001);  Plant callose synthase complexes.  Plant Mol. Biol.. 47 693-701
    CrossrefPubMedSuche in Google Scholar

U. Kutschera

Fachbereich Biologie
Universität Kassel

Heinrich-Plett-Straße 40
34109 Kassel
Germany

eMail: kut@uni-kassel.de

Section Editor: S. M. Wick