Physiological Comparisons among Four Related Bromus Species with Varying Ecological Amplitude: Polyamine and Aromatic Amine Composition in Response to Salt Spray and Drought

Abstract

We examined the amine composition in response to experimental osmotic or salt treatments of populations from four related Bromus species in relation to the environment of their origin. We studied populations from (1) a sand dune protected from salt spray, and (2) a sand dune subject to salt spray. The study of amine composition and contents, the first in Bromus, revealed a significant effect of origin for Spd and slightly for Spm, and higher levels were found in offspring of plants originating from an environment subject to sea spray. In all populations and species, plants subjected to osmotic and salt treatments showed significant decreases in Put, Spd, Spm and Dop levels. This was accompanied by significant increases in the oxidation product Dap and the amplitude of this response coincided with the presence of salt spray observed in the environment of origin of seeds, except for populations of B. rigidus. Interspecific differences in amine composition and contents were also detected. Higher levels of Tyr and Dop were found in B. sterilis and B. diandrus, respectively. For Dap and Tyr, B. rigidus, the species with the narrowest ecological amplitude in Brittany, showed the lowest response to either treatment. Our study of amine variations in Bromus populations and species illustrate the links between the amplitude of amine response to experimental salt or osmotic treatments and (1) the exposure of populations to sea spray; and (2) the ecological amplitude of Bromus species. Finally, experimental stress resulted in a strong decrease of interspecific variability in relation to amine metabolism in these four species.

Abbreviations

AA: ascorbic acid

AcCad: acetylcadaverine

APX: ascorbate peroxidase

Dap: 1,3-diaminopropane

Dop: dopamine

DW: dry weight

EDTA: ethylene diamine tetraacetic acid

FW: fresh weight

HPLC: high performance liquid chromatography

IAA: indol acetic acid

Oct: octopamine

Phe: phenethylamine

Put: putrescine

Spd: spermidine

Spm: spermine

Tyr: tyramine

Bromus L. - polyamine - aromatic amine - salt and osmotic treatments - populations - ecological amplitude

References

• 01 Ali,  R. M.. (2000);  Role of putrescine in salt tolerance of Atropa belladonna plant.  Plant Science. 152 173-179
• 02 Aziz,  A.,, Martin-Tanguy,  J.,, and Larher,  F.. (1998);  Stress-induced changes in polyamine and tyramine levels can regulate proline accumulation in tomato leaf discs treated with sodium chloride.  Physiologia Plantarum. 104 195-202
• 03 Bencsik,  K.,, Kremmer,  T.,, Boldizsar,  M.,, Tamas,  J.,, Mak,  M.,, and Paldi,  E.. (1998);  High-performance liquid chromatography determination and standardization of agmatine.  Journal of Chromatography A. 824 175-180
• 04 Blenau,  W., and Baumann,  A.. (2001);  Molecular and pharmacological properties of insect biogenic amine receptors: lessons from Drosophila melanogaster and Apis mellifera. .  Archives of Insect Biochemistry and Physiology. 48 13-38
• 05 Bonneau,  L.,, Carré,  M.,, Dreumont,  C.,, and Martin-Tanguy,  J.. (1994);  Polyamine metabolism during seedling development in rice.  Plant Growth Regulation. 15 83-92
• 06 Bouchereau,  A.,, Aziz,  A.,, Larher,  F.,, and Martin-Tanguy,  J.. (1999 a);  Polyamines and environmental challenges: recent development.  Plant Science. 140 103-125
• 07 Bouchereau,  A.,, Duhazé,  C.,, Martin-Tanguy,  J.,, Guégan,  J. P.,, and Larher,  F.. (1999 b);  Improved analytical methods for determination of nitrogenous stress metabolites occuring in Limonium species.  Journal of Chromatography A. 836 209-221
• 08 Debat,  V., and David,  P.. (2001);  Mapping phenotypes: canalization, plasticity and developmental stability.  Trends in Ecology and Evolution. 16 555-561
• 09 Des Abbayes,  H.,, Claustres,  G.,, Corillon,  R.,, and Dupont,  P.. (1971) Flore et végétation du Massif Armoricain. I. Flore vasculaire. Saint Brieuc; Presses Universitaires de Bretagne (ed.) p. 1226
• 10 Erdei,  L.,, Horvath,  F.,, Tari,  I.,, Pecsvaradi,  A.,, Szegletes,  Z.,, and Dulai,  S.. (2001);  Differences in photorespiration, glutamine synthetase and polyamines between fragmented and closed stands of Phragmites autralis. .  Aquatic Botany. 69 165-176
• 11 Esnault,  M. A.. (1984);  Etudes sur la variabilité morphologique de Bromus madritensis. .  Phytomorphology. 34 91-99
• 12 Esnault,  M. A., and Huon,  A.. (1987);  Etudes morphologiques et caryologiques de Bromus rigidus et Bromus diandrus Roth: relations taxomiques.  Bulletin de la Société Botanique Française. 134 299-304
• 13 Gagnieu,  A., and Laisne,  G.. (1949) La pratique des méthodes rapides en cytologie végétale. Paris; Imprimerie du Livre (ed.) 79 p.
• 14 Groppa,  M. A.,, Tomaro,  M. L.,, and Benavides,  M. P.. (2001);  Polyamines as protectors against cadmium or copper-induced oxidative damage in sunflower leaf discs.  Plant Science. 161 481-488
• 15 Guinaudeau,  H., and Bruneton,  J.. (1993) Isoquinoline alkaloids. Alkaloids and Sulphur Compounds/Methods in Plant Biochemistry, Vol. 8. Waterman, P. G., ed./Dey, P. M. and Harborne, J. B., eds. London; Academic Press pp. 373-419
• 16 Hennion,  F., and Martin-Tanguy,  J.. (1999);  Amine distribution and content in several parts of the subantarctic endemic species Lyallia kerguelensis (Hectorellaceae).  Phytochemistry. 52 247-251
• 17 Hennion,  F., and Martin-Tanguy,  J.. (2000);  Amines of the subantarctic crucifer Pringlea antiscorbutica are responsive to temperature conditions.  Physiologia Plantarum. 109 232-243
• 18 Hoagland,  D. R., and Arnon,  D. I.. (1938);  The water-culture method for growing plant without soil.  Californian Agricultural and Experimental Station Circ. 347 1-39
• 19 Kawano,  T.,, Pinontoan,  R.,, Uozumi,  N.,, Miyake,  C.,, Asada,  K.,, Kolattukudy,  P. E.,, and Muto,  S.. (2000);  Aromatic monoamine-induced immediate oxidative burst leading to an increase in cytosolic Ca²⁺ concentration in tobacco suspension culture.  Plant and Cell Physiology. 41 1251-1258
• 20 Lee,  T. M.,, Lur,  H. S.,, and Chu,  C.. (1997);  Role of abscisic acid in chilling tolerance of rice (Oryza sativa L.) seedlings. II Modulation of free polyamine levels.  Plant Science. 126 1-10
• 21 Leshem,  Y. Y., and Kuiper,  P. J. C.. (1996);  Is there a GAS (general adaptation syndrome) response to various types of environmental stress?.  Biologia Plantarum. 38 1-18
• 22 Lovaas,  E., and Olsen,  J. E.. (1998);  No induction of polyamines and radical scavenging antioxidants in Nicotiana tabaccum exposed to iron excess, as investigated by the DPPH assay and differential spectroscopy.  Journal of Plant Physiology. 153 401-408
• 23 Martin-Tanguy,  J.. (2001);  Metabolism and function of polyamines: recent development (new approaches).  Plant Growth and Regulation. 34 135-148
• 24 Oja,  T.. (2002);  Genetic divergence and intraspecific differentiation in the Bromus madritensis complex (Poaceae) based on isozyme data.  Biochemical Systematics and Ecology. 30 433-449
• 25 Oja,  T., and Jaaska,  V.. (1996);  Isoenzyme data on the genetic divergence and allopolypolidy in the section Genea of the grass genus Bromus (Poaceae).  Hereditas. 125 249-255
• 26 Pedrol,  N.,, Ramos,  P.,, and Reigosa,  M. J.. (2000);  Phenotypic plasticity and acclimatation to water deficits in velvet-grass: a long-term greenhouse experiment. Changes in leaf morphology, photosynthesis and stress-induced metabolites.  Journal of Plant Physiology. 157 383-393
• 27 Ponchet,  M.,, Martin-Tanguy,  J.,, Marais,  A.,, and Martin,  C.. (1982);  Hydroxycinnamoyl acid amines and aromatic amines in the inflorescences of some Araceae species.  Phytochemistry. 21 2865-2869
• 28 Racz,  I.,, Lasztity,  D.,, Darko,  E.,, Hidvégi,  E.,, and Szigeti,  Z.. (2000);  Paraquat resistance of horseweed (Erigeron canadensis L.) is not caused by polyamines.  Pesticide Biochemistry and Physiology. 68 1-10
• 29 Roy,  M., and Ghosh,  B.. (1996);  Polyamines, both common and uncommon, under heat stress in rice (Oryza sativa) callus.  Physiologia Plantarum. 98 196-200
• 30 Sharma,  M. L.. (1999);  Polyamine metabolism under abiotic stress at higher plants: salinity, drought and high temperature.  Physiology and Molecular Biology in Plants. 5 103-113
• 31 Shen,  W.,, Kazuyoshi,  N.,, and Tachibana,  S.. (2000);  Involvement of polyamines in the chilling tolerance of cucumber cultivars.  Plant Physiology. 124 431-439
• 32 Smekens,  M. J., and van Tienderen,  P. H.. (2001);  Genetic variation and plasticity of Plantago coronopus under saline conditions.  Acta Oecologica. 22 187-200
• 33 Smith,  T. A.. (1975);  Recent advances in the biochemistry of plant amines.  Phytochemistry. 14 865-890
• 34 Smith,  T. A., and Davies,  A. W.. (1985);  Separation and quantification of polyamines in plant tissue by high performance liquid chromatography of their dansyl derivates.  Plant Physiology. 78 89-91
• 35 Szopa,  J.,, Wilczylski,  G.,, Fiehn,  O.,, Wenczel,  A.,, and Willmitzer,  L.. (2001);  Identification and quantification of catecholamines in potato plants (Solanum tuberosum) by GC-MS.  Phytochemistry. 58 315-320
• 36 Tiburcio,  A. F.,, Kaur-Sawnay,  R.,, and Galston,  A. W.. (1990);  Polyamine metabolism.  The Biochemistry of Plants. 16 283-325
• 37 Turner,  L. B., and Stewart,  G. R.. (1986);  The effect of water stress upon polyamine levels in Barley (Hordeum vulgare L.) leaves.  Journal of Experimental Botany. 37 170-177
• 38 Vierling,  E., and Kimpel,  J. A.. (1992);  Plant responses to environmental stress.  Current Opinion in Biotechnology. 3 154-170

Section Editor: M. Riederer