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Plant Biol (Stuttg) 2003; 5(6): 608-614
DOI: 10.1055/s-2003-44689
Original Paper

Georg Thieme Verlag Stuttgart · New York

Genetic Isolation and Diffusion of Wild Grapevine Italian and Spanish Populations as Estimated by Nuclear and Chloroplast SSR Analysis

F. Grassi 1 , S. Imazio 1 , O. Failla 2 , A. Scienza 2 , R. Ocete Rubio 3 , M. A. Lopez 3 , F. Sala 1 , M. Labra 1
  • 1Department of Biology, University of Milan, Milano, Italy
  • 2Department of Crop Science, University of Milan, Via Celoria, 2, 201233 Milano, Italy
  • 3Laboratory of Applied Zoology, University of Sevilla, Avda Reina Mercedes, 641012 Sevilla, Spain
Further Information

Publication History

Publication Date:
02 February 2004 (online)

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Abstract

Genetic structure of six Italian and five Spanish populations of wild grapevine (Vitis vinifera ssp. silvestris) was investigated using nuclear and chloroplast SSR analysis. Results show that the Italian populations are characterised by high genomic diversity within populations, with a peak of heterozygosity (Ho = 0.7637) for a population collected in southern Italy. However, the low haplotype richness and the high level of genetic distance detected among the Spanish populations, combined with their low gene flow, shows that these populations suffered from a genetic erosion. Genetic relationship between Italian and Spanish populations was investigated and results showed genetic differentiation between the two populations. Using nuclear and chloroplast SSR markers, the ratio between pollen and seed diffusion was estimated. High pollen flow, as compared with seed flow, suggests that seed diffusion is made difficult, probably due to low germinability and survival of seedlings in the wild. Seed germination and young plant survival must be considered a priority target for in situ conservation programmes. By providing information on population history, genetic structure and gene flow, and by identifying areas harbouring high levels of wild grape variability, this study provides the basis for the preservation of biodiversity of the wild grapevine.

Key words

Biodiversity - chloroplast SSR - molecular tool - nuclear SSR - Vitis vinifera ssp. silvestris.

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F. Grassi

Dip. Biologia, Sez. Botanica Generale
University of Milan

Via Celoria 26

20133 Milano

Italy

Email: fabrizio.grassi@unimi.it

Section Editor: F. Salamini