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J Reconstr Microsurg 2005; 21(3): 191-196
DOI: 10.1055/s-2005-869826
BASIC SCIENCE REVIEW

Copyright © 2005 by Thieme Medical Publishers, Inc., 333 Seventh Avenue, New York, NY 10001, USA.

Evaluation of Fructose 1,6 diphosphate for Salvage of Ischemic Gracilis Flaps in Rats

Vipul Sud1 , Sheila G. Lindley2 , Olga McDaniel3 , Alan E. Freeland2 , Feng Zhang3 , Wanda Dorsett-Martin3 , Steven A. Bigler4 , William Lineaweaver1
  • 1Division Of Plastic Surgery, Department of Surgery, University of Mississippi Medical Center, Jackson, Mississippi
  • 2Department of Orthopedic Surgery and Division of Plastic Surgery, University of Mississippi Medical Center, Jackson, Mississippi
  • 3Department of Surgery, University of Mississippi Medical Center, Jackson, Mississippi
  • 4Department of Pathology, University of Mississippi Medical Center, Jackson, Mississippi
Further Information

Publication History

Accepted: October 8, 2004

Publication Date:
06 May 2005 (online)

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ABSTRACT

Fructose 1, 6 diphosphate (FDP), a metabolic intermediate, provides an alternative mechanism to circumvent the rate-limiting step in the Kreb's cycle. This agent has been observed to prevent the effects of ischemia on heart tissue and kidney function and the effects of endotoxic shock. It has been shown conclusively to minimize the adverse effects of ischemia-reperfusion injury in experimental pedicled skin flaps in animals. The present study was done to evaluate the effect of intra-arterial administration of FDP on salvage of ischemic microvascular transfer of gracilis muscle flaps in rats, with the premise that it might prolong the ischemia time of muscle flaps at room temperature, thus increasing chances of flap survival. Irrigation with FDP did not change the quantitative survival of the flaps, but there was qualitative improvement on histologic evaluation and DNA analysis. Decreased inflammatory damage and DNA fragmentation were seen at the 2.5-hr period. Histologic staining for mitochondrial oxygenation in gracilis muscle also showed increased uptake in the FDP-treated group vs. control at the 2.5-hr ischemia period. Further experiments with different modes of FDP administration should be carried out to identify more effective means of amelioration of flap ischemia.

KEYWORDS

Ischemia reperfusion injury - fructose diphosphate - microvascular transfer - gracilis muscle flaps in rats

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Sheila G LindleyM.D. 

Department of Orthopedic Surgery, University of Mississippi Medical Center

2500 N. State street, Jackson, MS 39216

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