J Reconstr Microsurg 1995; 11(3): 231-235
DOI: 10.1055/s-2007-1006537

© 1995 by Thieme Medical Publishers, Inc.

Characterization of Neurotrophic Activity in the Silicone-Chamber Model for Nerve Regeneration

Nils Danielsen, Silvio Varon
  • Departments of Experimental Research and Hand Surgery, Malmö University Hospital, University of Lund, Malmö, Sweden, and Department of Biology, University of California, San Diego, La Jolla, California
Further Information

Publication History

Accepted for publication 1994

Publication Date:
08 March 2008 (online)


In the present study, the authors reevaluated the temporal course and properties of neurotrophic activities present in the fluid accumulating in the silicone-chamber model for nerve regeneration. The fluid collected from silicone chambers was tested in four different dissociated neuronal cell cultures. Furthermore, the activity of the chamber fluid was examined, using a cell blot technique. There was one major peak of neurotrophic activity and this activity peaked early, about 3 to 6 hr after nerve injury. Results also indicate that the chamber fluid contains at least two types of neurotrophic activities, namely nerve growth factor and ciliary neurotrophic factor.