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DOI: 10.1055/s-2008-1082547
© Sonntag Verlag in MVS Medizinverlage Stuttgart GmbH & Co. KG
Beerenextrakte in Nahrungsergänzungsmitteln
Heikle Deklaration und Analytik der Anthocyane und Proanthocyanidine[*]Publikationsverlauf
Publikationsdatum:
24. Juli 2008 (online)

Zusammenfassung
Zubereitungen aus verschiedenen Beeren werden als Nahrungsergänzungsmittel mit unterschiedlichen »Health Claims« vermarktet, wobei die Effekte der enthaltenen Anthocyane und Proanthocyanidine hervorgehoben werden. Ein Vergleich der Präparate ist gegenwärtig aufgrund fehlender Vorgaben für die Deklaration und Problemen bei der Analytik der Polyphenole praktisch unmöglich. Diese Übersicht fasst Möglichkeiten für eine einheitlichere Erfassung der Inhaltsstoffe ausgewählter Beeren zusammen, um Ärzten, Apothekern und Konsumenten mehr Transparenz zu bieten.
Summary
Berry extracts in food supplements: tricky analysis of anthocyanins and proanthocyanidins for a proper declaration
Food supplements containing extracts from berries are marketed with different health claims underlining the effects of the anthocyanins and proanthocyanidins. Comparison of the products is almost impossible due to incomplete declarations and problems in the analyses of polyphenols. This overview compiles possibilities for a more uniform determination of these compounds in selected berries, which could provide better transparency to physicians, pharmacists and consumers.
Schlüsselwörter
Preiselbeere - Cranberry - Schwarze Holunderbeere - Kahle Apfelbeere - Schwarze Johannisbeere - Heidelbeere - Anthocyane - Proanthocyanidine - Analytik
Keywords
Lingonberry - cranberry - elderberry - chokeberry - black currant - bilberry - anthocyanins - proanthocyanidins - analytics
1 Gekürzte und aktualisierte Fassung der englischsprachigen Erstpublikation: Krenn L, Steitz M, Schlicht C, Kurth H, Gaedcke F. Anthocyanin- and proanthocyanidin-rich extracts of berries in food supplements – analysis with problems. Pharmazie 2007; 62: 803–812
Literatur
- 1
Krenn L, Steitz M, Schlicht C, et al..
Anthocyanin- and proanthocyanidin-rich extracts of berries in food supplements – analysis
with problems.
Pharmazie.
2007;
62
803-812
Reference Ris Wihthout Link
- 2
Kong JM, Chia LS, Goh NK, et al..
Analysis and biological activities of anthocyanins.
Phytochemistry.
2003;
64
923-933
Reference Ris Wihthout Link
- 3
Wrolstadt RE, Durst RW, Giusti MM, Rodriguez-Saona LE.
Analysis of anthocyanins in nutraceuticals.
ACS Symposium Series.
2002;
803 (Quality Management of Nutraceuticals)
42-62
Reference Ris Wihthout Link
- 4
Lea AGH, Arnold GM.
The phenolics of ciders: Bitterness and adstringency.
J Sci Food Agric.
1978;
29
478-483
Reference Ris Wihthout Link
- 5
Prior RL, Gu L.
Occurrence and biological significance of proanthocyanidins in the American diet.
Phytochemistry.
2005;
66
2264-2280
Reference Ris Wihthout Link
- 6 Rohr GE, Meier B, Sticher O.
Analysis of procyanidins . In: Rahman AU, ed. Studies in Natural Products Chemistry, Vol. 21 (Bioactive Natural Products – Part B). Amsterdam; Elsevier Science 2000: 497-570Reference Ris Wihthout Link - 7
Gu L, Kelm M, Hammerstone JF, et al..
Screening of foods containing proanthocyanidins and their structural characterization
using LC-MS/MS and thiolytic degradation.
J Agric Food Chem.
2003;
51
7513-7521
Reference Ris Wihthout Link
- 8
Beattie J, Crozier A, Duthie GG.
Potential health benefits of berries.
Current Nutrition & Food Science.
2005;
1
71-86
Reference Ris Wihthout Link
- 9
Santos-Buelga C, Scalbert A.
Proanthocyanidins and tannin-like compounds – nature, occurrence, dietary intake and
effects on nutrition and health.
J Sci Food Agric.
2000;
80
1094-1117
Reference Ris Wihthout Link
- 10
Rasmussen SE, Frederiksen H, Struntze Krogholm K, Poulsen L.
Dietary proanthocanidins: Occurrence, dietary intake, bioavailability, and protection
against cardiovascular disease.
Mol Nutr Food Res.
2005;
49
150-174
Reference Ris Wihthout Link
- 11
Wu X, Beecher GR, Holden JM, et al..
Concentrations of anthocyanins in common foods in the United States and estimation
of normal consumption.
J Agric Food Chem.
2006;
54
4069-4075
Reference Ris Wihthout Link
- 12
Zheng W, Wang SY.
Oxygen radical absorbing capacity of phenolics in blueberries, cranberries, chokeberries,
and lingonberries.
J Agric Food Chem.
2003;
51
502-509
Reference Ris Wihthout Link
- 13
Kähkönen MP, Hopia AI, Heinonen M.
Berry phenolics and their antioxidant activity.
J Agric Food Chem.
2001;
49
4076-4082
Reference Ris Wihthout Link
- 14
Kähkönen MP, Heinämäki J, Ollilainen V, Heinonen M.
Berry anthocyanins: isolation, identification and antioxidant activities.
J Sci Food Agric.
2003;
83
1403-1411
Reference Ris Wihthout Link
- 15
Bomser J, Madhavi DL, Singletary K, Smith MA.
In vitro anticancer activity of fruit extracts from Vaccinium species.
Planta Med.
1996;
62
212-216
Reference Ris Wihthout Link
- 16
Foo LY, Lu Y, Howell AB, Vorsa N.
The structure of cranberry proanthocyanidins which inhibit adherence of uropathogenic
P-fimbriated Escherichia coli in vitro.
Phytochemistry.
2000;
54
173-181
Reference Ris Wihthout Link
- 17
Malien-Aubert C, Dangles O, Amiot MJ.
Color stability of commercial anthocyaninbased extracts in relation to the phenolic
composition. Protective effects by intra- and intermolecular copigmentation.
J Agric Food
Chem.
2001;
49
170-176
Reference Ris Wihthout Link
- 18
Strigl AW, Leitner E, Pfannhauser W.
Qualitative und quantitative Analyse der Anthocyane in Schwarzen Apfelbeeren (Aronia melanocarpa Michx. Ell.) mittels TLC, HPLC und UV/VIS-Spektrometrie.
Z Lebensm Unters Forsch.
1995;
201
266-268
Reference Ris Wihthout Link
- 19
Määttä KR, Kamal-Eldin A, Törrönen AR.
High-performance liquid chromatography (HPLC) analysis of phenolic compounds in berries
with diode array and electrospray ionization mass spectrometric (MS) detection: Ribes species.
J Agric Food Chem.
2003;
51
6736-6744
Reference Ris Wihthout Link
- 20
Määttä K, Kamal-Eldin A, Törrönen R.
Phenolic compounds in berries of black, red, green, and white currants (Ribes sp.)
Antioxidants & Redox Signaling.
2001;
3
981-993
Reference Ris Wihthout Link
- 21
Canter PH, Ernst E.
Anthocyanosides of Vaccinium myrtillus (bilberry) for night vision – a systematic review of placebocontrolled trials.
Surv Ophthalmol.
2004;
49
38-50
Reference Ris Wihthout Link
- 22 ESCOP .
Myrtilli fructus . ESCOP Monographs. 2nd ed. Exeter: European Scientific Cooperative on Phytotherapy. 2003: 345-350Reference Ris Wihthout Link - 23
Cunningham DG, Vannozzi S, O’Shea E, Turk R.
Analysis and standardization of cranberry products.
ACS Symposium Series.
2002;
803 (Quality Management of Nutraceuticals)
151-166
Reference Ris Wihthout Link
- 24
Avorn J, Monane M, Gurwitz JH, et al..
Reduction of bacteriuria and pyuria after ingestion of cranberry juice.
JAMA.
1994;
271
751-754
Reference Ris Wihthout Link
- 25
Kontiokari T, Sundqvist K, Nuutinen M, et al..
Randomised trial of cranberry-lingonberry juice and Lactobacillus GG drink for the prevention of urinary tract infections in women.
BMJ (Clinical Research ED.).
2001;
322
1-5
Reference Ris Wihthout Link
- 26
Stothers L.
A randomized trial to evaluate effectiveness and cost effectiveness of naturopathic
cranberry products as prophylaxis against urinary tract infection in women.
Can J Urol.
2002;
9
1558-1562
Reference Ris Wihthout Link
- 27
Bailey DT, Dalton C, Daugherty FJ, Tempesta MS.
Can a concentrated cranberry extract prevent recurrent urinary tract infections in
women? A pilot study.
Phytomedicine.
2007;
14
237-241
Reference Ris Wihthout Link
- 28
Zafriri D, Ofek I, Adar R, et al..
Inhibitory activity of cranberry juice on adherence of type 1 and type P fimbriated
Escherichia coli to eucaryotic cells.
Antimicrob Agents Chemother.
1989;
33
92-98
Reference Ris Wihthout Link
- 29
Ahuja S, Kaack B, Roberts J.
Loss of fimbrial adhesion with the addition of Vaccinium macrocarpon to the growth medium of P-fimbriated Escherichia coli
.
J Urology.
1998;
159
559-562
Reference Ris Wihthout Link
- 30
Foo LY, Lu Y, Howell AB, Vorsa N.
A-type proanthocyanidin trimers from cranberry that inhibit adherence of uropathogenic
P-fimbriated Escherichia coli
.
J Nat Prod.
2000;
63
1225-1228
Reference Ris Wihthout Link
- 31
Zirk MM, Aluko RE, Taylor CG.
Cranberry (Vaccinium macrocarpon) proanthocyanidins and their effects on urinary tract infections.
Curr Topics Nutraceutical Res.
2004;
2
153-159
Reference Ris Wihthout Link
- 32
Di Martino P, Agniel R, David K, et al..
Reduction of Escherichia coli adherence to uroepithelial bladder cells after consumption of cranberry juice: a
double-blind, randomized placebo-controlled cross-over trial.
World J Urol.
2006;
24
21-27
Reference Ris Wihthout Link
- 33
Liu Y, Black MA, Caron L, Camesano TA.
Role of cranberry juice on molecular-scale surface characteristics and adhesion behaviour
of Escherichia coli
.
Biotechnol Bioengeneering.
2006;
93
297-305
Reference Ris Wihthout Link
- 34
Gupta K, Chou MY, Howell A, et al..
Cranberry products inhibit adherence of P-fimbriated Escherichia coli to primary cultured bladder and vaginal epithelial cells.
J Urol.
2007;
177
2357-2360
Reference Ris Wihthout Link
- 35
Hui J, Choy J, Suwandaratne SP, et al..
Antimicrobial activity of Vaccinium macrocarpon (cranberry) produced proanthocyanidin (PAC) on the growth and adhesion properties
of Staphylococcus aureus
.
J Food Nutr.
2004;
9
29-33
Reference Ris Wihthout Link
- 36
Howell AB, Reed JD, Krueger CG, et al..
A-type cranberry proanthocyanidins and uropathogenic bacterial anti-adhesion activity.
Phytochemistry.
2005;
66
2281-2291
Reference Ris Wihthout Link
- 37
Zhang L, Ma J, Pan K, et al..
Efficacy of cranberry juice on Helicobacter pylori infection: a double-blind, randomized placebo-controlled trial.
Helicobacter.
2005;
10
139-145
Reference Ris Wihthout Link
- 38
Shmuely H, Yahav J, Samra Z, et al..
Effect of cranberry juice on eradication of Helicobacter pylori in patients treated with antibiotics and a proton pump inhibitor.
Mol Nutr Food Res.
2007;
51
746-751
Reference Ris Wihthout Link
- 39
Ruel G, Pomerleau S, Couture P, Lemieux S.
Favourable impact of low-calorie cranberry juice consumption on plasma HDL-cholesterol
concentrations in men.
Br J Nutr.
2006;
96
357-364
Reference Ris Wihthout Link
- 40
Cheng HY, Lin TC, Yang CM, et al..
In vitro anti-HSV-2 activity and mechanism of action of proanthocyanidin A-1 from
Vaccinium vitis-idaea
.
J Sci Food Agric.
2005;
85
10-15
Reference Ris Wihthout Link
- 41
Wu X, Gu L, Prior RL, McKay S.
Characterization of
anthocyanins and proanthocyanidins in some cultivars of Ribes, Aronia, and Sambucus and their antioxidant capacity.
J Agric Food Chem.
2004;
52
7846-7856
Reference Ris Wihthout Link
- 42
Bermudez-Soto MJ, Tomas-Barberan FA.
Evaluation of commercial red fruit juice concentrates as ingredients for antioxidant
functional juices.
Eur Food Res Technol.
2004;
219
133-141
Reference Ris Wihthout Link
- 43
Oszmianski J, Wojdylo A.
Aronia melanocarpa phenolics and their antioxidant activity.
Eur Food Res Technol.
2005;
221
809-813
Reference Ris Wihthout Link
- 44
Matsumoto M, Hara H, Chiji H, Kasai T.
Gastroprotective effect of red pigments in black chokeberry fruit (Aronia melanocarpa Elliot) on acute gastric hemorrhagic lesion in rats.
J Agric Food Sci.
2004;
52
2226-2229
Reference Ris Wihthout Link
- 45
Niedworok J, Jankowska B, Kowalczyk E, et al..
Antiulcer activity of anthocyanin from Aronia melanocarpa Elliot.
Herba Polonica.
1997;
43
222-227
Reference Ris Wihthout Link
- 46
Valcheva-Kuzmanova S, Borisova P, Galunska B, et al..
Hepatoprotective effect of the natural fruit juice from Aronia melanocarpa on carbon tetrachloride-induced acute liver damage in rats.
Exp Toxicol Pathol.
2004;
56
195-201
Reference Ris Wihthout Link
- 47
Valcheva-Kuzmanova S, Marazova K, Krasnaliev I, et al..
Effect of Aronia melanocarpa fruit juice on indomethacin-induced gastric mucosal damage and oxidative stress in
rats.
Exp Toxicol Pathol.
2005;
56
385-392
Reference Ris Wihthout Link
- 48
Valcheva-Kuzmanova S, Kuzmanov K, Mihova V, et al..
Antihyperlipidemic effect of Aronia melanocarpa fruit juice in rats fed a high-cholesterol diet.
Plant Foods Hum Nutr.
2007;
62
19-24
Reference Ris Wihthout Link
- 49
Valcheva-Kuzmanova S, Kuzmanov K, Tancheva S, Belcheva A.
Hypoglycemic and hypolipidemic effects of Aronia melanocarpa fruit juice in streptozotocininduced diabetic rats.
Methods Find Exp Clin Pharmacol.
2007;
29
101-105
Reference Ris Wihthout Link
- 50
Naruszewicz M, Laniewska I, Millo B, Dluzniewski M.
Combination therapy of statin with flavonoids rich extract from chokeberry fruits
enhanced reduction in cardiovascular risk markers in patients after myocardial infarction
(MI).
Atherosclerosis.
2007;
194
e179-e184
Reference Ris Wihthout Link
- 51
Benvenuti S, Pellati F, Melegari M, Bertelli D.
Polyphenols, anthocyanins, ascorbic acid, and radical scavenging activity of Rubus, Ribes, and Aronia
.
J Food Sci.
2004;
69
164-169
Reference Ris Wihthout Link
- 52
Knox YM, Hayashi K, Suzutani T, et al..
Activity of anthocyanins from fruit extracts of Ribes nigrum L. against influenza A and B viruses.
Acta Virologica.
2001;
45
209-215
Reference Ris Wihthout Link
- 53
Knox YM, Suzutani T, Yosida I, Azuma M.
Anti-influenza virus activity of crude extract of Ribes nigrum L.
Phytother Res.
2003;
17
120-122
Reference Ris Wihthout Link
- 54
Suzutani T, Ogasawara M, Yoshida I, et al..
Anti-herpesvirus activity of an extract of Ribes nigrum L.
Phytother Res.
2003;
17
609-613
Reference Ris Wihthout Link
- 55
Zakay-Rones Z, Varsano N, Zlotnik M, et al..
Inhibition of several strains of influenza virus in vitro and reduction of symptoms
by an elderberry extract (Sambucus nigra L.) during an outbreak of influenza B Panama.
J Altern Complement Med.
1995;
1
361-369
Reference Ris Wihthout Link
- 56
Zakay-Rones Z, Thom E, Wollan T, Wadstein J.
Randomized study of the efficacy and safety of oral elderberry extract in the treatment
of Influenza A and B virus infections.
J Int Med Res.
2004;
2
132-140
Reference Ris Wihthout Link
- 57
Barak V, Halperin T, Kalickman I.
The effect of sambucol, a black elderberry-based, natural product, on the production
of human cytokines: I. Inflammatory cytokines.
European Cytokine Network.
2001;
12
290-296
Reference Ris Wihthout Link
- 58
Barak V, Birkenfeld S, Halperin T, Kalickman I.
The effect of herbal remedies on the production of human inflammatory and antiinflammatory
cytokines.
Israel Med Assoc J.
2002;
4
919-922
Reference Ris Wihthout Link
- 59
Schofield P, Mbugua DM, Pell AN.
Analysis of condensed tannins: a review.
Anim Feed Sci Tech.
2001;
91
21-40
Reference Ris Wihthout Link
- 60
Prodanov MP, Dominguez JA, Blazquez I, et al..
Some aspects of quantitative/ qualitative assessment of commercial anthocyanin-rich
extracts.
Food Chem.
2005;
90
585-596
Reference Ris Wihthout Link
- 61
Wu X, Prior R.
Systematic identification and characterization of anthocyanins by HPLC-ESI-MS/MS in
common foods in the United States: fruits and berries.
J Agric Food Chem.
2005;
53
2589-2599
Reference Ris Wihthout Link
- 62
Nakajima J, Tanaka I, Seo S, et al..
LC/PDA/ESI-MS profiling and radical scavenging activity of anthocyanins in various
berries.
J Biomed Biotech.
2004;
5
241-247
Reference Ris Wihthout Link
- 63
Määttä-Riihinen KR, Kähkönen MP, Törrönen AR, Heinonen IM.
Catechins and procyanidins in berries of Vaccinium species and their antioxidant activity.
J Agric Food Chem.
2005;
53
8485-8491
Reference Ris Wihthout Link
- 64
Gu L, Kelm M, Hammerstone JF, et al..
Fractionation of polymeric procyanidins from lowbush blueberry and quantification
of
procyanidins in selected foods with an optimized normal-phase HPLC-MS fluorescent
detection
method.
J Agric Food Chem.
2002;
50
4852-4860
Reference Ris Wihthout Link
1 Gekürzte und aktualisierte Fassung der englischsprachigen Erstpublikation: Krenn L, Steitz M, Schlicht C, Kurth H, Gaedcke F. Anthocyanin- and proanthocyanidin-rich extracts of berries in food supplements – analysis with problems. Pharmazie 2007; 62: 803–812
2 Die Früchte des Holunders sind eigentlich Steinfrüchte und jene der Apfelbeere Sammelbalgfrüchte.
ao. Univ.-Prof. Dr. Liselotte Krenn
Institut für Pharmakognosie
Universität Wien
PharmaCenterVienna
Althanstraße 14
A-1090 Wien
Österreich