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J Brachial Plex Peripher Nerve Inj 2010; 05(01): e105-e115
DOI: 10.1186/1749-7221-5-18
Research article
Li et al; licensee BioMed Central Ltd.

Functional cooperation of of IL-1β and RGS4 in the brachial plexus avulsion mediated brain reorganization[*]

Jifeng Li
1   Lab of Hand function reconstruction, Huashan Hospital, Fudan University, Shanghai, China
,
Hui Zhao
1   Lab of Hand function reconstruction, Huashan Hospital, Fudan University, Shanghai, China
,
Pengbo Luo
1   Lab of Hand function reconstruction, Huashan Hospital, Fudan University, Shanghai, China
,
Yudong Gu
1   Lab of Hand function reconstruction, Huashan Hospital, Fudan University, Shanghai, China
› Author Affiliations
Subject Editor:
Further Information

Publication History

04 June 2010

07 December 2010

Publication Date:
19 September 2014 (online)

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Abstract

Backgrounds There is considerable evidence that central nervous system is continuously modulated by activity, behavior and skill acquisition. This study is to examine the reorganization in cortical and subcortical regions in response to brachial plexus avulsion.

Methods Adult C57BL/6 mice were divided into four groups: control, 1, 3 and 6 month of brachial plexus avulsion. IL-1β, IL-6 and RGS4 expression in cortex, brainstem and spinal cord were detected by BiostarM-140 s microarray and real-time PCR. RGS4 subcellular distribution and modulation were further analyzed by primary neuron culture and Western Blot.

Results After 1, 3 and 6 months of brachial plexus avulsion, 49 (0 up, 49 down), 29 (17 up, 12 down), 13 (9 up, 4 down) genes in cerebral cortex, 40 (8 up, 32 down), 11 (7 up, 4 down), 137 (63 up, 74 down) in brainstem, 27 (14 up, 13 down), 33 (18 up, 15 down), 60 (29 up, 31 down) in spinal cord were identified. Among the regulated gene, IL-1β and IL-6 were sustainable enhanced in brain stem, while PKACβ and RGS4 were up-regulated throughout cerebral cortex, brainstem and spinal cord in 3 and 6 month of nerve injury. Intriguingly, subcellular distribution of RGS4 in above three regions was dependent on the functional correlation of PKA and IL-1β.

Conclusion Data herein indicated that brachial plexus avulsion could efficiently initiate and perpetuate the brain reorganization. Network involved IL-1β and RGS4 signaling might implicate in the re-establish and strengthening of the local circuits at the cortical and subcortical levels.

*This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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