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Drug Res (Stuttg) 2022; 72(05): 259-267
DOI: 10.1055/a-1785-4005
Original Article

Celecoxib has Preventive and Therapeutic Benefits against Nephrotoxicity Caused by Gentamicin in Mice

Ahmed M. Abd-Eldayem
1   Department of Pharmacology, Faculty of Medicine, Assiut University, Assiut, Egypt
2   Al-Ghad International Colleges of Applied Medical Sciences, Abha, Saudi Arabia
,
Marwa A. Dahpy
3   Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Assiut University, Assiut, Egypt
4   Department of Medical Biochemistry and Molecular Biology, Armed Forces College of Medicine, Cairo, Egypt
,
Dalia M. Badary
5   Department of Pathology, Faculty of Medicine, Assiut University, Assiut, Egypt
,
Sulaiman Mohammed Alnasser
6   Department of Pharmacology and Toxicology, Unaizah College of Pharmacy, Qassim University, Qassim, Saudi Arabia
,
Mohammad Salem Hareedy
1   Department of Pharmacology, Faculty of Medicine, Assiut University, Assiut, Egypt
› Author Affiliations Funding This research received no specific grant from any funding agency in the public, commercial, private, or not-for-profit sectors.
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Abstract

It’s crucial to comprehend the impact of oxidative stress and pro-inflammatory cytokines in the gentamicin-induced kidney injury mechanism. Celecoxib was administered orally either before or after intraperitoneal therapy with gentamicin in mice. The serum levels of creatinine (SCr), blood urea nitrogen (BUN), IL-6, and TNF-α were measured by ELISA test, as well as the levels of the kidney tissue malondialdehyde (MDA), and glutathione (GSH) were also estimated spectrophotometrically. The renal expression of nuclear factor-κB (NF-κB), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and cyclooxygenase 2 (COX-2) mRNAs were evaluated by qPCR. Histopathological evaluation and Immunohistochemical examination of kidney NF-κB, IL-6, and COX-2 were also, performed. Celecoxib successfully prevented gentamicin-induced kidney damage as indicated by reducing blood BUN, SCr, and tissue MDA levels and increasing renal tissue GSH levels as well as lowering the blood IL-6 and TNF-α in comparison to mice received gentamicin. Furthermore, celecoxib has inhibited COX-2, NF-κB, IL-6, and TNF-α expression in the renal tissue. It is noteworthy that celecoxib therapy after gentamicin administration brought about substantially the same results as celecoxib treatment before gentamicin injection in mice. Our results showed the role of celecoxib as a therapeutic tool for gentamicin-induced nephrotoxicity as well as raised its beneficial prophylactic role in this medical challenge by attenuating oxidative stress and inflammation.

Keywords

Celecoxib - Gentamicin - Nephrotoxicity - Oxidative stress - Inflammatory markers - Mice.


Publication History

Received: 21 January 2022

Accepted: 28 February 2022

Article published online:
31 March 2022

© 2022. Thieme. All rights reserved.

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