Monitoring Biocatalytic Transformations Mediated by Polyketide Synthase Enzymes in Cell Lysate via Fluorine NMR

Abstract

The biocatalytic employment of modular polyketide synthase enzymes in cell lysate has become a viable route to preparative quantities of synthetically valuable polyketide fragments. We report the quantitative, uninvasive, and continuous monitoring of such biocatalytic reactions by observing trifluoromethyl-bearing substrates via ¹⁹F NMR spectroscopic analysis. To demonstrate the utility of this technique, we followed reactions catalyzed by a thioesterase and several ketoreductases.

Key words

biosynthesis - biocatalysis - polyketide synthase enzymes - polyketide - fluorine NMR

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