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DOI: 10.1055/s-0032-1331225
Synthesis and Biological Evaluation of a Novel Series of Pyrazolylcoumarins as Anti-Inflammatory and Antioxidant Agents
Synthesis of 3-(1H-pyrazol-5-yl)-2H-chromen-2-one derivativesCorrespondence
Publication History
received 10 July 2012
accepted 13 November 2012
Publication Date:
08 January 2013 (online)
- Abstract
- Introduction
- Experimental
- Pharmacological Evaluation
- Results and Discussion
- Conclusion
- References
Abstract
A new series of pyrazolylcoumarins (4a-k) was synthesized by reaction of appropriate dibromochalcones (3a-k) with phenyl hydrazine in ethanol. Structures of all new synthesized compounds were characterized on the basis of elemental analysis and spectral data (UV, IR and 1H NMR). The title compounds were screened for in vivo anti-inflammatory activities at a dose of 200 mg/kg b.w. Among the 11 prepared compounds, Compounds 4c, d, h and i exhibited significant anti-inflammatory activity in model of acute inflammation such as carrageenan-induced rat edema paw while compound 4d showed considerable activity in model of chronic inflammation such as adjuvant-induced arthritis along with minimum ulcerogenic index and were compared with diclofenac (13.5 mg/kg b.w.) as a standard drug. Additionally, the synthesized compounds were evaluated for their in vitro antioxidant activity. Compound 4k was found to be the most active antioxidant in the series compared with standard vitamin C.
#
Introduction
Nonsteroidal anti-inflammatory drugs (NSAIDs) have been recognized as important class of therapeutic agents for the alleviation of pain and inflammation associated with a number of pathological conditions. However, long term use of NSAIDs has been associated with several side effects such as gastrointestinal mucosal damage, bleeding, intolerance and renal toxicity [1] [2] [3] [4]. Thus, there is an urgent need for new targets that are required for the design and development of novel anti-inflammatory agents as an alternative to NSAIDs. Extensive research has been directed towards improving their pharmacological profile that led to the discovery of multiple isoforms of cyclooxygenase (COX) that are differently regulated [5] [6]. The discovery of the inducible isoform of cyclooxygenase enzyme (COX-2) spurred the search for anti-inflammatory agents devoid of the undesirable effects associated with classical NSAIDs. Recently, a novel class of selective COX-2 inhibitors has been discovered. Among this class, celecoxib was shown to be a potent and gastrointestinal (GI) safe anti-inflammatory analgesic agent. It is considered a typical model of pyrazole containing, diaryl-heterocyclic template that is known to selectively inhibit COX-2 [7]. Furthermore, several other compounds containing pyrazole functionality were also reported to exhibit anti-inflammatory activity [8] [9] [10] [11] [12].
Reactive oxygen species (ROS) are implicated in the induction and prolongation of inflammatory process [13]. Interestingly a number of therapeutically useful NSAID’s have been shown to act by virtue of their free radical scavenging activity[14] [15] [16]. Antioxidants are the compounds that prevent oxidative damage induced by free radicals and ROS. Thus, antioxidant therapy has also gained immense importance in the treatment of the above mentioned diseases [17].
Coumarin derivatives are an interesting class of heterocyclic systems, since coumarin ring consist an essential core moiety for a variety of natural and synthetic biologically active compounds [18] [19] [20] [21]. The biological activities include anticoagulation, antibiotic, antifungal, antipsoriasis, antitumor, anti-HIV, anti-inflammatory, etc. Moreover, since the combination of 2 pharmacophores on the same scaffold is a well established approach to the synthesis of more potent drugs, we decided to incorporate the pyrazole ring moiety in the coumarin derivatives and to examine the influence of this modification on their activities as anti-inflammatory and free radical scavengers.
#
Experimental
All research chemicals were purchased from Sigma-Aldrich (St. Louis, Missouri, USA) or Lancaster Co. (Ward Hill, MA, USA) and used as such for the reactions. Solvents except laboratory reagent grade were dried and purified according to the literature when necessary.
Reactions were monitored by thin-layer chromatography (TLC) on pre-coated silica gel plates from E. Merck and Co. (Darmstadt, Germany). Melting points of synthesized compounds were determined in Thermonik (Mumbai, India) melting point apparatus and are uncorrected, UV spectra were recorded on Thermospectronic (Rochester, NY, USA) and IR spectra were recorded on Thermo Nicolet IR200 FT-IR Spectrometer (Madison, WI, USA) by using KBr pellets. The 1HNMR were recorded on Bruker AVANCE 300 (Bruker, Rheinstetten/Karlsruhe, Germany) using DMSO-d6 as solvent. Chemical shifts are reported in δ ppm units with respect to TMS as internal standard. The purity of compounds was examined by TLC on silica gel plate using chloroform and methanol (10:1) as mobile phase and iodine vapours as visualizing agent. The anti-inflammatory activity was carried out using digital plethysmometer (Ugo-Basile, Italy). A series of 3-[3-(substituted phenyl)-1-phenyl-1H-pyrazol-5-yl]-2H-chromen-2-one (4a-k) was synthesized by earlier reported method [23]. The physico-chemical and spectral data of synthesized compounds 4a-k is summarized in [Table 1] [2] respectively.
Compound |
R a |
Yield (%) |
M. P. (0 C) |
Rf b |
Formula |
M. W. |
---|---|---|---|---|---|---|
a All compounds purified by Column Chromatography using Chloroform:Methanol (10:1) as mobile phase; b Chloroform:Methanol 10:1 as a mobile phase and iodine vapours as visualizing agent |
||||||
4 a |
H- |
53 |
158–260 |
0.38 |
C24H16N2O2 |
364 |
4 b |
4-OMe- |
83 |
120–122 |
0.27 |
C25H18N2O3 |
394 |
4 c |
4-Cl- |
76 |
98–100 |
0.48 |
C24H15ClN2O2 |
398 |
4 d |
2,4-(Cl)2- |
92 |
126–130 |
0.24 |
C24H14 Cl2N2O2 |
433 |
4 e |
4-NMe2- |
70 |
148–150 |
0.46 |
C26H21N3O2 |
407 |
4 f |
3-NO2- |
67 |
100–102 |
0.25 |
C24H15N3O4 |
409 |
4 g |
4-Me- |
74 |
90–92 |
0.36 |
C24H18N2O2 |
378 |
4 h |
3-OMe- |
83 |
136–138 |
0.32 |
C25H18N2O3 |
394 |
4 i |
4-F- |
64 |
190–192 |
0.29 |
C24H15FN2O2 |
382 |
4 j |
2-NO2- |
71 |
208–210 |
0.33 |
C24H15N3O4 |
409 |
4 k |
4-OH- |
76 |
182–184 |
0.42 |
C24H16N2O3 |
380 |
Compd |
R |
UV (CH3OH) |
IR (KBr, cm-1) |
1H-NMR (DMSO-d6 δ, ppm) |
Elemental Analysis (CHN) |
---|---|---|---|---|---|
4 a |
H- |
λmax 290 |
1679.0 (coumarin, C=O), 1634.74 (C=N), 1491.00 (C=C). |
8.13 (s, 1H, 4-H of coumarin) 7.35–8.11 (m, 14H, Ar-H), 7.16 (s, 1H, 4-H of pyrazole). |
Anal. Calcd for C24H16N2O2
|
4 b |
4-OMe- |
λmax 272 |
1681.97 (coumarin, C=O), 1624.74 (C=N), 1490.05 (C=C). |
8.13 (s, 1H, 4-H of coumarin), 7.01–7.88 (m, 13H, Ar-H), 7.15 (s, 1H, 4-H of pyrazole), 3.81 (s, 3H, -OCH3). |
Anal. Calcd for C25H18N2O3
|
4 c |
4-Cl- |
λmax 302 |
1682.05 (coumarin, C=O), 1614.43 (C=N), 1486.72 (C=C). |
8.15 (s, 1H, 4-H of coumarin), 7.31–8.13 (m, 13H, Ar-H), 7.27 (s, 1H, 4-H of pyrazole). |
Anal. Calcd for C24H15ClN2O2
|
4 d |
2,4-(Cl)2- |
λmax 286 |
1682.63 (coumarin, C=O), 1616.31 (C=N), 1484.97 (C=C). |
8.15 (s, 1H, 4-H of coumarin), 7.31–8.08 (m, 12H, Ar-H), 7.28 (s, 1H, 4-H of pyrazole). |
Anal. Calcd for C24H14Cl2N2O2
|
4 e |
4-NMe2- |
λmax 276 |
1673.65 (coumarin, C=O), 1604.94 (C=N), 1449.36 (C=C). |
8.13 (s, 1H, 4-H of coumarin), 6.77–7.88 (m, 13H, Ar-H), 7.19 (s, 1H, 4-H of pyrazole), 3.02 (s, 6H, -N(CH3)2). |
Anal. Calcd for C26H21N3O2
|
4 f |
3-NO2- |
λmax 286 |
1685.23 (coumarin, C=O), 1611.11 (C=N), 1449.36 (C=C). |
8.69 (s, 1H, 4-H of coumarin), 7.16–7.88 (m, 13H, Ar-H), 7.14 (s, 1H, 4-H of pyrazole). |
Anal. Calcd for C24H15N3O4
|
4 g |
4-Me- |
λmax 282 |
1715.41 (coumarin, C=O), 1672.78 (C=N), 1552.98 (C=C). |
8.13 (s, 1H, 4-H of coumarin), 7.12–7.88 (m, 13H, Ar-H), 7.23 (s, 1H, 4-H of pyrazole), 2.34 (s, 3H, -CH3). |
Anal. Calcd for C25H18N2O2
|
4 h |
3-OMe- |
λmax 292 |
1681.97 (coumarin, C=O), 1624.94 (C=N), 1490.05 (C=C). |
8.13 (s, 1H, 4-H of coumarin), 7.02–7.88 (m, 13H, Ar-H), 7.26 (s, 1H, 4-H of pyrazole), 3.81 (s, 3H, -OCH3). |
Anal. Calcd for C25H18N2O3
|
4 i |
4-F- |
λmax 312 |
1682.05 (coumarin, C=O), 1614.43 (C=N), 1486.72 (C=C). |
8.13 (s, 1H, 4-H of coumarin), 7.28–7.88 (m, 13H, Ar-H), 7.16 (s, 1H, 4-H of pyrazole). |
Anal. Calcd for C24H15FN2O2
|
4 j |
2-NO2- |
λmax 276 |
1689.61 (coumarin, C=O), 1609.40 (C=N), 1458.55 (C=C). |
8.16 (s, 1H, 4-H of coumarin), 7.37–8.13 (m, 13H, Ar-H), 6.99 (s, 1H, 4-H of pyrazole). |
Anal. Calcd for C24H15N3O4
|
4 k |
4-OH- |
λmax 274 |
3208.00 (-OH), 1671.33 (coumarin, C=O), 1607.79,1505.92 (C=C). |
9.46 (s, 1H, -OH), 8.13 (s, 1H, 4-H of coumarin), 6.85–7.66 (m, 13H, Ar-H), 7.15 (s, 1H, 4-H of pyrazole) |
Anal. Calcd for C24H16N2O3
|
#
Pharmacological Evaluation
Albino mice of either sex weighing 20–25 g were used for acute toxicity studies and analgesic activity. Healthy male albino adult rats weighing 150–230 g were used for various pharmacological screenings. Animals were procured from Department of Pharmacology, RVS College of Pharmaceutical Sciences, Sulur, Tamilnadu, India, (1012/C/06/CPCSEA) and housed individually in polypropylene cages, maintained under standard conditions of alternating 12 h light and dark cycles at a constant temperature (25±2°C and 35–60% relative humidity). Animals were fed with standard rat pellet diet, (Hindustan Lever Ltd., Mumbai, India) and water ad libitum.
Acute toxicity [24]
The acute toxicity test was carried out according to the Organization for Economic Co-operation and Development (OECD) guidelines to establish the effective dose of test compounds after obtaining ethical clearance from Animal Ethics Committee of RVS College of Pharmaceutical Sciences, Sulur (India). Albino mice of either sex weighing between 20 and 25 g were grouped into 12 groups of 6 animals each, starved for 24 h with water ad libitum prior to test. On the day of the experiment animals were administered with different compounds to different groups in an increasing dose of 10, 20, 100, 200, 1 000 and 2 000 mg/kg body weight orally. The animals were then observed continuously for 3 h for general behavioral, neurological, autonomic profiles and then every 30 min for next 3 h and finally for next 24 h or till death.
#
Acute anti-inflammatory activity
In vivo acute anti-inflammatory activity was evaluated using carrageenan-induced rat paw edema assay model of inflammation by adopting the method of Winter et al. [25] for the compounds listed in [Table 3]. Male albino rats (170–220 g) were fasted with free access to water at least 12 h prior to experiments and were divided randomly into 13 groups of 6 each. Control group received 1 ml of 0.5% sodium carboxymethyl cellulose (sodium CMC), standard group received 13.5 mg/kg of diclofenac and test groups received 200 mg/kg of synthesized compounds (4a-k). The rats were dosed orally, 1 h later; a subplantar injection of 0.05 ml of 1% solution of carrageenan in sterile distilled water was administered to the left hind footpad of each animal. The paw edema volume was measured with a digital plethysmometer at 0, 1, 2, 3, 4, 5 h after carrageenan injection. Paw edema volume was compared with vehicle control group and percent reduction was calculated as 1-(edema volume in the drug treated group/edema volume in the control group)×100.
Compound |
R |
Paw edema volume in ml (% inhibition) |
|||||
---|---|---|---|---|---|---|---|
0 h |
1 h |
2 h |
3 h |
4 h |
5 h |
||
The results are expressed as mean±SEM (n=6). Each value in parenthesis indicates the percentage inhibition. Significance was calculated by using one-way ANOVA. The difference in results was considered significant when p<0.05. *p<0.05 vs. control at 200 mg/kg b.w; **p<0.01 vs. control at 200 mg/kg b.w; ***p<0.01 vs. control at 13.5 mg/kg b.w |
|||||||
Control |
0.50±0.026 |
0.89±0.02 |
1.11±0.030 |
1.18±0.012 |
1.26±0.008 |
1.22±0.007 |
|
4a |
H- |
0.47±0.020 |
0.81±0.019 |
0.92±0.011 |
0.84±0.019 |
0.87±0.021 |
0.81±0.021 |
4b |
4-OMe- |
0.46±0.009 |
0.78±0.017 |
0.90±0.014 |
0.94±0.012 |
0.88±0.021 |
0.81±0.019 |
4c |
4-Cl |
0.45±0.021 |
0.76±0.019 |
0.79±0.019 |
0.76±0.015 |
0.64±0.019 |
0.55±0.017 |
4d |
2,4-(Cl)2 |
0.48±0.018 |
0.80±0.030 |
0.74±0.019 |
0.69±0.024 |
0.64±0.024 |
0.61±0.023 |
4e |
4-NMe2 |
0.49±0.04 |
0.79±0.010 |
0.78±0.014 |
0.71±0.013 |
0.66±0.017 |
0.61±0.017 |
4f |
3-NO2 |
0.48±0.016 |
0.82±0.029 |
0.88±0.021 |
0.84±0.019 |
0.82±0.020 |
0.78±0.019 |
4g |
4-Me |
0.47±0.019 |
0.80±0.022 |
0.93±0.011 |
0.85±0.017 |
0.83±0.020 |
0.82±0.024 |
4h |
3-OMe |
0.46±0.017 |
0.81±0.017 |
0.76±0.017 |
0.71±0.018 |
0.63±0.013 |
0.54±0.026 |
4i |
4-F |
0.44±0.013 |
0.75±0.017 |
0.79±0.010 |
0.75±0.014 |
0.63±0.016 |
0.50±0.013 |
4j |
2-NO2 |
0.49±0.019 |
0.79±0.023 |
0.92±0.014 |
0.88±0.014 |
0.84±0.019 |
0.83±0.019 |
4k |
4-OH |
0.48±0.015 |
0.76±0.025 |
0.88±0.021 |
0.80±0.025 |
0.74±±0.026 |
0.67±0.024 |
Diclofenac |
0.50±0.022 |
0.76±0.014 |
0.79±0.017 |
0.59±0.018 |
0.46±0.020 |
0.41±0.013 |
#
Chronic anti-inflammatory activity
In vivo chronic anti-inflammatory activity was assessed using adjuvant-induced arthritis assay model of inflammation by utilizing the earlier reported method of Newbould [26]. Normally fed male albino rats weighing between 150 and 230 g were used for the experiment. On day 1, 0.1 ml of heat-killed Mycobacterium tuberculosis (Freund’s adjuvant complete) was injected into the left hind footpad of each rat. The rats were kept in cages for 15 days. On day 15, all animals with ‘developed’ arthritis were used for the study and were divided into 6 groups of 6 rats each for various treatments as shown in [Table 4]. Control group received 1 ml of 0.5% sodium CMC, standard group received 13.5 mg/kg b.w. of diclofenac. Test groups received 200 mg/kg b.w. of selected compounds 4c, d, h and i. All compounds were administered orally from day 16 to 19. The hind paw volumes, body weight and degree of secondary lesions were recorded daily from day 16 to 19. The decrease or increase, in mean paw volume per group per day was calculated as a percent inhibition from day 15 onwards.
Compound |
Anti-inflammatory activitya |
||
---|---|---|---|
Paw edema volume (mean±SEM) |
% Inhibition after treatment (on day 19) |
||
Day 15b |
Day 19c |
||
The results are expressed as mean±SEM (n=6). Significance was calculated by using one-way ANOVA with Dunnet’s t- test. The difference in results was considered significant when p<0.05. *p<0.01 vs. control at 200 mg/kg b.w; **p<0.01 vs. control at 13.5 mg/kg b.w. |
|||
a Inhibitory activity in Freund’s adjuvant-induced rat paw edema |
|||
b Paw edema volume before treatment |
|||
c Paw edema volume after treatment |
|||
Control |
0.97±0.028 |
0.88±0.018 |
|
4c |
0.85±0.030 |
0.52±0.026 |
41.34±2.52* |
4d |
0.86±0.020 |
0.48±0.027 |
45.28±2.73* |
4h |
0.82±0.018 |
0.68±0.020 |
23.43±2.74* |
4i |
0.85±0.028 |
0.61±0.022 |
30.70±2.33* |
Diclofenac |
0.81±0.025 |
0.39±0.020 |
55.67±2.18** |
#
Ulcerogenic activity [27]
Albino rats of either sex were divided into control, standard and different test groups of 6 animals each group (170–250 g). They were starved for 48 h (water ad libitum) prior to drug administration. Control group received only 0.5% sodium CMC solution, standard group was orally administered with acetylsalicylic acid in sodium CMC solution and test compounds 4c, d, h and i were administered orally at the dose of 200 mg/kg b.w. All animals were sacrificed after 7 h of drug administration. Stomach was removed and placed on saline-soaked filter paper until inspection. A longitudinal incision along the greater curvature was made with fine scissors. The stomach was everted over the index finger and the presence or absence of gastric irritationwas determined. The ulcer index for each group was determined according to a previously reported method [36] by counting the number of lesions (x) in each of 5 size classes (y). The classes were defined as y=1 (pinpoint lesion), y=2 (lesions<1 mm diameter), y=3 (lesions 1–2 mm diameter), y=4 (lesions 2–4 mm diameter) and y=5 (lesions>4 mm diameter). The ulcer index was calculated using ∑5i=1 xiyi
#
Antioxidant Activity
Free radical scavenging activity of compounds was determined using 1,1-diphenyl-2-picryl hydrazyl (DPPH) free radical [28]. Briefly, 2 mL samples of various concentrations (100–1 000 μg/ml) were added to 2 mL of 100 μM DPPH solution. After 20 min incubation at room temperature, the absorbance was read against a blank at 517 nm. The change in absorbance with respect to the control (containing DPPH only without sample, expressed as 100% free radicals) was calculated as percentage scavenging using following the equation:·
(A517blank − A517sample)÷A517blank×100%.·
The reading was taken in triplicate and mean used for calculation of IC50. The IC50 (mean±SEM) stand for the concentration required for 50% inhibition of DPPH radicals and was calculated from ORIGIN PC version 6.0 software.
#
Statistical analysis
Data are presented as arithmetic mean±SEM. Statistical analysis was performed by one way variance (ANOVA) followed by Dunnett’s test,“p” value of less than 0.05 was considered as statistically significant using GRAPHPAD PRISM PC version 4.03 by GraphPad software INC-2005.
#
#
Results and Discussion
Synthesis
The synthesis of 3-[3-(substituted phenyl)-1-phenyl-1H-pyrazol-5-yl]-2H-chromen-2-one (4a-k) was carried out as presented in [Fig. 1]. Starting material 3-acetyl-2H-chromen-2-one 1 was synthesized by the reaction of salicylaldehyde with ethylacetoacetate in the presence of a catalytic amount of piperidine at room temperature following the literature procedure [22]. 3-[(2E)-3-(substitutedphenyl)prop-2-enoyl]-2H-chromen-2-one (chalcones, 2a-k) were synthesized by Claisen–Schmidt condensation of 3-acetyl-2H-chromen- 2-one 1 with various substituted benzaldehydes in the presence of a mixture of piperidine and n-butanol. Efforts to convert compounds 2a-k into target molecules 4a-k under a variety of conditions were not successful. Hence, an alternative method was adopted. This involved the bromination of chalcones 2a-k and subsequent ring closure using phenyl hydrazine. Bromination of chalcones 2a-k was carried out in chloroform using bromine in chloroform to yield dibromo compounds 3a-k, which were cyclized with phenyl hydrazine in the presence of triethylamine in absolute ethanol to afford the 3-[3-(substituted phenyl)-1-phenyl-1H-pyrazol-5-yl]-2H-chromen-2-one 4a-k. The physicochemical and spectral analysis data of the synthesized compounds are depicted in [Table 1] [2], respectively.
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#
Acute toxicity study
From the preliminary toxicity studies, it was observed that, all the test compounds have revealed good safety profile till the uppermost dose (2 000 mg/kg). No mortality of animals observed even after 24 h but there were few changes in the behavioral response like alertness, touch response and restlessness. Therefore, 1/10th of the maximum tolerated dose i. e., 200 mg/kg b.w. was chosen for the various pharmacological evaluations.
#
Acute anti-inflammatory activity
[Table 3] reveals the in vivo acute anti-inflammatory activity of a novel series of 3-[3-(substituted phenyl)-1-phenyl-1H-pyrazol-5-yl]-2H-chromen-2-one (4a-k) at a dose of 200 mg/kg in carrageenan-induced paw edema method. Carrageenan-induced edema is a non-specific inflammation resulting from a complex of diverse mediators. Since edema of this type is highly sensitive to NSAIDs, carrageenan has been accepted as a useful agent for studying new anti-inflammatory agents. This model reliably predicts the anti-inflammatory efficacy of the NSAIDs, and during the second phase it detects compounds that are anti-inflammatory agents as a result of inhibition of prostaglandin amplification [35]. As shown in [Table 3], the entire investigated compounds exhibited moderate to good anti-inflammatory activity with the percentage inhibition of edema formation ranged from 31.51 to 66.43, while the reference drug diclofenac (13.5 mg/kg) showed 66.43% inhibition at fifth hour. Compound 4c (35.1 and 54.57%) and 4d (41.60 and 50.06%) showed good inhibitory activity at third and fifth hour respectively, while the most active compounds 4i (35.82 and 58.52%) and 4h (39.63 and 55.25%) among the series, exhibited the excellent inhibitory activity at third and fifth hour, respectively. The anti-inflammatory activity of these compounds was comparable with that of the standard drug diclofenac, although not equal. Compounds that showed significant anti-inflammatory activity profile were further tested for chronic anti-inflammatory and ulcerogenic activities.
#
Chronic anti-inflammatory activity
[Table 4] explains the in vivo chronic anti-inflammatory activity of compounds 4c, d, h and i in adjuvant-induced arthritis in rats. Injection of Freund’s complete adjuvant to the hind footpad in rats produced arthritis and rats with developed arthritis were chosen for the study. On day 15, there were not many variations in the edema volumes of different groups. Administration of 4c, d, h and i at a dose of 200 mg/kg and diclofenac (13.5 mg/kg) for 3 days (from day 16 to day 19) in rats has produced significant reduction in paw volume (by day 19) when compared to the arthritic control group. Compounds 4c (41.32%), 4d (45.28%) and 4i (30.75%) were found to possess a substantial inhibition of edema formation when compared to 4h (23.58%) while diclofenac (55.66%) exhibited significant anti-inflammatory activity.
#
Ulcerogenic activity
The major drawback of NSAIDs is their gastric ulcer formation due to gastric irritation. The extent of ulcerogenic effect was evaluated for compounds 4c, d, h and i in rat stress model at the therapeutic dose (i. e., 200 mg/kg b.w.). The gastric ulcerogenic potential was evaluated by calculating the ulcer index in treated and control animals. Results are given in [Table 5] that indicates these four compounds (ulcer index ranges from 1.8 to 4.6) cause less gastric ulceration and disruption of gastric epithelial cells at the abovementioned oral dose as compared to acetylsalicylic acid (ulcer index 5.1). Hence gastric tolerance to these compounds was better than that of standard drug.
Compound |
Ulcer Index (±SEM) |
---|---|
The results are expressed as mean±SEM (n=6). Data analysed by one-way ANOVA with Dunnet’s t-test. *p<0.05 significant from control; **p<0.01 significant from control |
|
Control |
1.4 (±3.10) |
4c |
1.8 (±0.42) |
4d |
2.67 (±0.28)* |
4h |
2.12 (±0.11) |
4i |
4.6 (±1.02) |
Acetylsalicylic acid |
5.1 (±0.88)** |
#
Antioxidant activity
It has been reported that scavenging of ROS might be beneficial in reducing the development of inflammation. In order to determine the extent of scavenging effect, all compounds were tested for antioxidant activity using 1,1-diphenyl-2-picryl hydrazyl (DPPH) free radical. All compounds showed antioxidant activity ([Table 6]). Compounds 4d and k showed 1.03±0.067 and 1.07±0.088 IC50 as compared with standard ascorbic acid (0.026±0.022 mM) and butylated hydroxy toluene (0.13±0.012 mM).
Compound |
DPPH scavenging activity IC50 (±SEM) (mM)a |
---|---|
a The results are expressed as IC50±SEM (n=3) (mM), the concentration of the test compound that provides 50% scavenging of the DPPH radicals already available in the solution. Butylated hydroxy toluene (BHT) and Ascorbic acid (ASC) are used as the standard reference |
|
4 a |
1.77±0.012 |
4 b |
1.51±0.012 |
4 c |
1.27±0.133 |
4 d |
1.03±0.067 |
4 e |
1.23±0.016 |
4 f |
1.15±0.045 |
4 g |
1.37±0.167 |
4 h |
1.59±0.023 |
4 i |
1.32±0.059 |
4 j |
1.52±0.025 |
4 k |
1.07±0.080 |
Butylated Hydroxy Toluene. |
0.13±0.012 |
Ascorbic Acid |
0.026±0.022 |
#
#
Conclusion
The objective of the present study was to synthesize and investigate the anti-inflammatory and antioxidant activities of a new series of pyrazolylcoumarins with the hope of discovering new structure leads serving as dual anti-inflammatory–antiantioxidant agents. The substitution pattern of the pyrazole ring was rationalized so as to be correlated to the vicinal diaryl heterocycles template. Amongst the tested compounds, 4h and 4i showed pronounced acute anti-inflammatory activity (55 and 58% respectively) that was comparable to diclofenac (66%), whereas, compounds 4c and 4d displayed good anti-inflammatory activity (54 and 50% respectively) while compound 4d showed considerable activity in model of chronic inflammation such as adjuvant-induced arthritis.. However, none of the newly synthesized compounds was found to be superior over the reference drug. In addition, the tested compounds were also found to possess less degree of ulcerogenic potential as compared to aspirin. Compounds 4d and 4k having dichloro and hydroxyl moiety demonstrated maximum antioxidant activity.
The compound with p-chlorophenyl (4c) substitution was found to be active among tested compounds as anti-inflammatory as well as antioxidant agents, amd less degree of ulcerogenic potential. Here strong electron withdrawing (–Cl) substitution at ortho and para position in phenyl ring responsible for activity. This shows that the antioxidant activity influence the anti-inflammatory activity. Finally compound 4c could be identified as the most biologically active member within this study with an interesting dual anti-inflammatory and antioxidant profile. Further in-vitro COX-1 and 2 inhibitory activity of pyrazolylcoumarins class needs to be investigated using celecoxib as standard with the hope of finding new anti-inflammatory agent.
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Conflict of Interest
Authors hereby declare that there are no financial/commercial conflicts of interest.
Acknowledgement
The authors are thankful to Principal, RVS College of Pharmaceutical Science, Sulur, Coimbtore We are grateful to The Director, SAIF, Punjab University for providing spectral analysis.
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References
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- 13 Freeman B, Crop J. Biology of disease, free radicals and tissue injury. Lab Invest 1982; 47: 412-426
- 14 Vane J. The mode of action of asprin-like drugs. Agents React 1978; 6: 430-431
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- 16 Roberfroid M, Viche H, Remede J. Free Radicals in Drug Research. In: Testa B. (ed.). adv. Drug Research Res. Vol 16. Academic Press; London: 1987: 2-43
- 17 Velavan S, Naghlendran K, Mahesh R et al. In vitro antioxidant activity of Asparagus racemosus root. Phcogmag 1998; 26-32
- 18 Kennedy R, Thornes R. Coumarins: Biology, Applications and Mode of Action. Wiley & Sons; Chichester, UK: 1997
- 19 Murray RDH, Mendez J, Brown SA. The Natural Coumarins, Occurrence, Chemistry and Biochemistry. John Wiely & Sons; New York: 1982
- 20 Fylaktakidou KC, Hadjipavlou-Litina DJ, Litinas KE et al. Natural and Synthetic Coumarin Derivatives with Antiinflammatory/Antioxidant Activities. Curr Pharm Design 2004; 10: 3813-3833
- 21 Lacy A, O’Kennedy R. Studies on coumarins and coumarin-related compounds to determine their therapeutic role in the treatment of cancer. Curr Pharm Des 2004; 10: 3797-3811
- 22 Knoevenagel E. Knoevenagel Condensation. Chem Ber 1898; 31: 2596
- 23 Aragade P, Venkatnarayanan R, Maddi VS et al. J. Synthesis and antibacterial activity of a new series of 3-[3-(substituted phenyl)-1-phenyl-1H-pyrazol-5-yl]-2H-chromen-2-one derivative. Enzy Inhib Med Chem 2012; 27: 849-853
- 24 OECD/OCDC, OECD ‘‘Guidelines for Testing of Chemicals’’ Revised Draft Guidelines 423; Acute Oral Toxicity Class Method, Revised Document, October 2000
- 25 Winter CA, Risley EA, Nuss GW. Carrageenin-induced edema in hind paw of the rat as an assay for antiiflammatory drugs. Proc Soc Exp Biol Med 1962; 111: 544-547
- 26 Newbould BB. Chemotherapy of arthritis induced in rats by Mycobacterial adjuvant. Br j pharmacol chemother 1963; 21: 127-136
- 27 Vogel HG. (ed.). Drug Discovery and Evaluation: Pharmacological Assays. third ed. Springer-Verlag; Berlin, Heidelberg: 2002
- 28 Blois MS. Antioxidant determination by the use of a stable free radical. Nature 1958; 181: 1199-1200
Correspondence
-
References
- 1 Sontag SJ. Prostaglandins in peptic ulcer disease: An overview of current status and future directions. Drug 1986; 32: 445-457
- 2 Allison MC, Howatson AG, Torrance CJ et al. Gastrointestinal damage associated with the use of nonsteroidal antiinflammatory drugs. N Engl J Med 1992; 327: 749-754
- 3 Flower RJ. The development of COX2 inhibitors. Nature Reviews Drug Discovery 2003; 2: 179-191
- 4 Clive DM, Stoff JS. Renal syndromes associated with non-steroidal antiinflammatory drugs. N Engl J Med 1984; 310: 563-570
- 5 Song Y, Connor DT, Doubleday R et al. Synthesis, structure activity relationship and in-vivo evaluation of substituted ditetrabutyl phenols as a novel class of potent selective and orally active cyclooxygenase-2 inhibitors, 1-thiazolone and Oxazolone series. J Med Chem 1999; 42: 1151-1160
- 6 Chandrasekharan NV, Dai H, Roos KLT et al. COX-3, a cyclooxygenase-1 variant inhibited by acetaminophen and other analgesic/antipyretic drugs: cloning, structure and expression. Proc Natl Acad Sci USA 2002; 99: 13926-13931
- 7 Palomer A, Cabré F, Pascual J et al. Identification of novel cyclooxygenase-2 selective inhibitors using pharmacophore models. J Med Chem 2002; 45 (07) 1402-1411
- 8 Gadad AK, Kittur BS, Kapsi SG et al. Synthesis, analgesic and anti-inflammatory activities of some 1-acyl/aracyl-5-aminopyrazole derivatives. Arzneim Forsch 1996; 46: 1082-1085
- 9 Penning TD, Talley JJ et al. Synthesis and biological evaluation of the 1,5-diarylpyrazole class of cyclooxygenase-2 inhibitors: Identification of 4-5-(4-methylphenyl)-3-(trifluoromethyl)-1 H-pyrazol-1-yl]benzenesulfonamide (SC-58635, Celecoxib). J Med Chem 1997; 40: 1347-1365
- 10 Tsuji K, Nakamura K, Ogino T et al. Studies on anti-inflammatory agents. VI. Synthesis and pharmacological properties of 2,3-diarylthiophenes. Chem Pharm Bull 1998; 46: 279-286
- 11 Connolly PJ, Wetter SK, Beers KN et al. N-hydroxyurea and hydroxamic acid inhibitors of cyclooxygenase and 5-lipoxygenase. Bioorg Med Chem Lett 1999; 9: 979-986
- 12 Boschelli D, Connor D, Bornemeier DA et al. 1,3,4-oxadiazole, 1, 3, 4-thiadiazole, and 1, 2, 4-triazole analogs of the fenamates: In vitro inhibition of cyclooxygenase and 5-lipoxygenase activities. J Med Chem 1993; 36: 1802-1810
- 13 Freeman B, Crop J. Biology of disease, free radicals and tissue injury. Lab Invest 1982; 47: 412-426
- 14 Vane J. The mode of action of asprin-like drugs. Agents React 1978; 6: 430-431
- 15 Halliwell B, Gutteridge JMC. Free radicals in biology and medicine. 2nd ed. Oxford: Clarendon Press; 1989: 427
- 16 Roberfroid M, Viche H, Remede J. Free Radicals in Drug Research. In: Testa B. (ed.). adv. Drug Research Res. Vol 16. Academic Press; London: 1987: 2-43
- 17 Velavan S, Naghlendran K, Mahesh R et al. In vitro antioxidant activity of Asparagus racemosus root. Phcogmag 1998; 26-32
- 18 Kennedy R, Thornes R. Coumarins: Biology, Applications and Mode of Action. Wiley & Sons; Chichester, UK: 1997
- 19 Murray RDH, Mendez J, Brown SA. The Natural Coumarins, Occurrence, Chemistry and Biochemistry. John Wiely & Sons; New York: 1982
- 20 Fylaktakidou KC, Hadjipavlou-Litina DJ, Litinas KE et al. Natural and Synthetic Coumarin Derivatives with Antiinflammatory/Antioxidant Activities. Curr Pharm Design 2004; 10: 3813-3833
- 21 Lacy A, O’Kennedy R. Studies on coumarins and coumarin-related compounds to determine their therapeutic role in the treatment of cancer. Curr Pharm Des 2004; 10: 3797-3811
- 22 Knoevenagel E. Knoevenagel Condensation. Chem Ber 1898; 31: 2596
- 23 Aragade P, Venkatnarayanan R, Maddi VS et al. J. Synthesis and antibacterial activity of a new series of 3-[3-(substituted phenyl)-1-phenyl-1H-pyrazol-5-yl]-2H-chromen-2-one derivative. Enzy Inhib Med Chem 2012; 27: 849-853
- 24 OECD/OCDC, OECD ‘‘Guidelines for Testing of Chemicals’’ Revised Draft Guidelines 423; Acute Oral Toxicity Class Method, Revised Document, October 2000
- 25 Winter CA, Risley EA, Nuss GW. Carrageenin-induced edema in hind paw of the rat as an assay for antiiflammatory drugs. Proc Soc Exp Biol Med 1962; 111: 544-547
- 26 Newbould BB. Chemotherapy of arthritis induced in rats by Mycobacterial adjuvant. Br j pharmacol chemother 1963; 21: 127-136
- 27 Vogel HG. (ed.). Drug Discovery and Evaluation: Pharmacological Assays. third ed. Springer-Verlag; Berlin, Heidelberg: 2002
- 28 Blois MS. Antioxidant determination by the use of a stable free radical. Nature 1958; 181: 1199-1200
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