Abstract
New, simple and cost effective infrared spectroscopic method has been developed for
the estimation of zidovudine (CAS 30516-87-1) in bulk and tablet dosage form. The
quantitative analysis of zidovudine was carried out in solid form using KBr pellet
method and in liquid form using quartz cuvette. These methods were developed and validated
for various parameters according to ICH guidelines. Linearity range was found to be
0.8–1.6% w/w in KBr pellet method and 250–1 500 μg ml−1 in solution. The proposed methods were successfully applied for the determination
of zidovudine in pharmaceutical formulation (tablets). The results demonstrated that
the proposed methods are accurate, precise and reproducible (relative standard deviation<2%),
while being simple, economical and less time consuming than other available methods
and can be used for estimation of zidovudine in different dosage forms.
Key words
CAS 30516-87-1 - zidovudine quantitative estimation - method validation - infrared
spectroscopy - KBr pellet method - quartz cuvette method
