Remote Postconditioning Attenuates Ischemia/Reperfusion Injury in Rat Skeletal Muscle through Mitochondrial ATP-Sensitive K+ Channel–Dependent Mechanism

Jung Il Lee; Kyung Wook Nha; Jin Soo Suh; Suk Kyu Choo; Jung Ho Park; Jong Woong Park

doi:10.1055/s-0033-1348037

Journal of Reconstructive Microsurgery, Table of Contents

J Reconstr Microsurg 2013; 29(09): 571-578
DOI: 10.1055/s-0033-1348037

Original Article

Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

Remote Postconditioning Attenuates Ischemia/Reperfusion Injury in Rat Skeletal Muscle through Mitochondrial ATP-Sensitive K⁺ Channel–Dependent Mechanism

Jung Il Lee

¹Department of Orthopedic Surgery, Inje University Ilsan Paik Hospital, Goyang, Korea

,

Kyung Wook Nha

¹Department of Orthopedic Surgery, Inje University Ilsan Paik Hospital, Goyang, Korea

,

Jin Soo Suh

¹Department of Orthopedic Surgery, Inje University Ilsan Paik Hospital, Goyang, Korea

,

Suk Kyu Choo

¹Department of Orthopedic Surgery, Inje University Ilsan Paik Hospital, Goyang, Korea

,

Jung Ho Park

²Department of Orthopedic Surgery, Korea University Ansan Hospital, Ansan, Korea

,

Jong Woong Park

²Department of Orthopedic Surgery, Korea University Ansan Hospital, Ansan, Korea

› Author Affiliations

Abstract

The authors investigated whether a remote postconditioning (remote post-con) procedure attenuated skeletal muscle ischemia/reperfusion (I/R) injury. We determined the optimal protocol of remote post-con and investigated its mechanism. Ischemia was induced for 3 hours in rat left hindlimb and three protocols of remote post-con were applied in right hindlimb just before the end of ischemia. The first (10-second group) involved two cycles of 10 seconds of occlusion followed by 10 seconds of reperfusion. The second (5-minute group) involved two cycles of 5 minutes of occlusion/reperfusion. The third (10-minute group) involved two cycles of 10 minutes of occlusion/reperfusion. In 5- and 10-minute groups, wet/dry ratio and muscle fiber edema were significantly lower than control group. Muscle contractility was preserved in 5- and 10-minute groups. An injection of 5-hydroxydecanoate (a specific blocker of mitochondrial ATP-sensitive K⁺ [mK_ATP] channels) impaired this effect. This study demonstrates that remote post-con preserves muscle contractility and reduces tissue edema and necrosis, possibly through the activation of mK_ATP channels. We suggest that two cycles of 5 minutes of occlusion followed by 5 minutes of reperfusion are optimal protocols of remote post-con in skeletal muscle I/R injury.

Keywords

Ischemia/reperfusion injury - postconditioning - remote postconditioning - ATP-sensitive K⁺ channel

Full Text

References

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