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Horm Metab Res 2015; 47(01): 72-77
DOI: 10.1055/s-0034-1390489
Immediate Interest
© Georg Thieme Verlag KG Stuttgart · New York

Modification of Human Islet Preparation: An Effective Approach to Improve Graft Outcome After Islet Transplantation?

M. Zhao
1   Department of Diabetes and Endocrinology, Division of Diabetes and Nutrients King’s College London, London, UK
,
P. Choudhary
1   Department of Diabetes and Endocrinology, Division of Diabetes and Nutrients King’s College London, London, UK
,
P. Srinivasan
2   Institute of Liver Studies, King’s College Hospital, London, UK
,
H. Tang
1   Department of Diabetes and Endocrinology, Division of Diabetes and Nutrients King’s College London, London, UK
,
N. Heaton
2   Institute of Liver Studies, King’s College Hospital, London, UK
,
M. Fung
1   Department of Diabetes and Endocrinology, Division of Diabetes and Nutrients King’s College London, London, UK
,
A. Barthel
3   Department of Medicine III, Dresden, Germany
4   Endokrinologikum RUHR, Bochum, Germany
,
S. R. Bornstein
1   Department of Diabetes and Endocrinology, Division of Diabetes and Nutrients King’s College London, London, UK
3   Department of Medicine III, Dresden, Germany
5   Centre for Diabetes Research, Paul Langerhans Institute Dresden, Dresden, Germany
,
S. A. Amiel
1   Department of Diabetes and Endocrinology, Division of Diabetes and Nutrients King’s College London, London, UK
,
G. C. Huang
1   Department of Diabetes and Endocrinology, Division of Diabetes and Nutrients King’s College London, London, UK
› Author Affiliations
Further Information

Publication History

received 18 August 2014

accepted 22 September 2014

Publication Date:
05 November 2014 (online)

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Abstract

Revascularisation of transplanted islets is an essential prerequisite for graft survival and function. However, current islet isolation procedures deprive the islets of endothelial tubulets. This may have a detrimental effect on the revascularisation process of islets following transplantation. We hypothesise that modification of the isolation procedure that preserves islet endothelial vessels may improve the islet revascularisation process following transplantation. Here, we present a modified islet isolation method by which a substantial amount of endothelial cells still attached to the islets could be preserved. The islets with preserved endothelial cells isolated by this method were revascularised within 3 days, not observed in islets isolated by standard methods. Further, we observed that grafts of islets isolated by standard methods had more patches of dead tissue than islet grafts obtained by the modified method, indicating that attached endothelial cells may play an important role in the islet revascularisation process and potentially help to improve the transplantation outcome.

Key words

human islet - transplantation - endothelium cells - revascularisation
 

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