Purkinje Cell Dendritic Atrophy Induced by Prenatal Stress Is Mitigated by Early Environmental Enrichment

Rodrigo Pascual; Martina Valencia; Carlos Bustamante

doi:10.1055/s-0034-1395344

Neuropediatrics, Table of Contents

Neuropediatrics 2015; 46(01): 037-043
DOI: 10.1055/s-0034-1395344

Original Article

Georg Thieme Verlag KG Stuttgart · New York

Purkinje Cell Dendritic Atrophy Induced by Prenatal Stress Is Mitigated by Early Environmental Enrichment

Rodrigo Pascual

¹Laboratorio de Neurociencias, Escuela de Kinesiología, Facultad de Ciencias, Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile

,

Martina Valencia

¹Laboratorio de Neurociencias, Escuela de Kinesiología, Facultad de Ciencias, Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile

,

Carlos Bustamante

¹Laboratorio de Neurociencias, Escuela de Kinesiología, Facultad de Ciencias, Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile

› Author Affiliations

Abstract

Background Prenatal stress (PS) in experimental animals causes long-lasting changes in Purkinje cell dendritic morphology. Furthermore, these structural changes are associated with an increase in anxiogenic behaviors in the elevated plus maze (EPM) and open-field (OF) test.

Objectives As environmental enrichment (EE) has significant restorative effects on brain neurons and behavior, the aim of this study was to evaluate if postweaning EE mitigates the decrease in Purkinje cell dendritic expansion and exploratory behavior induced by PS in mice.

Materials and Methods Restraint stress was induced from gestational day 14 (G14) to G21. Approximately 50% of the PS animals were submitted to the EE paradigm between postnatal days 22 (P22) and P52. At P52 and P82, male animals were behaviorally evaluated, and then the morphology of the cerebellar vermal Purkinje cells was analyzed.

Results We found that EE significantly ameliorates the Purkinje cell dendritic atrophy and anxiety-like behavior in the EPM.

Conclusion Our data show that long-lasting Purkinje cell dendritic impairments and anxiety-like behavior can be mitigated by postweaning EE.

Keywords

prenatal stress - Purkinje cells - environmental enrichment - fetal programming

Full Text

References

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