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Thromb Haemost 2001; 85(01): 36-41
DOI: 10.1055/s-0037-1612660
Review Article
Schattauer GmbH

Anti-αvβ3 Antibodies in Chronic Immune Thrombocytopenic Purpura

Satoru Kosugi
1   Department of Internal Medicine and Molecular Science, Graduate School of Medicine, Osaka University, Osaka, and Department of Blood Transfusion, Osaka University Hospital, Osaka, Japan
,
Yoshiaki Tomiyama
1   Department of Internal Medicine and Molecular Science, Graduate School of Medicine, Osaka University, Osaka, and Department of Blood Transfusion, Osaka University Hospital, Osaka, Japan
,
Shigenori Honda
1   Department of Internal Medicine and Molecular Science, Graduate School of Medicine, Osaka University, Osaka, and Department of Blood Transfusion, Osaka University Hospital, Osaka, Japan
,
Hirokazu Kashiwagi
1   Department of Internal Medicine and Molecular Science, Graduate School of Medicine, Osaka University, Osaka, and Department of Blood Transfusion, Osaka University Hospital, Osaka, Japan
,
Masamichi Shiraga
1   Department of Internal Medicine and Molecular Science, Graduate School of Medicine, Osaka University, Osaka, and Department of Blood Transfusion, Osaka University Hospital, Osaka, Japan
,
Seiji Tadokoro
1   Department of Internal Medicine and Molecular Science, Graduate School of Medicine, Osaka University, Osaka, and Department of Blood Transfusion, Osaka University Hospital, Osaka, Japan
,
Teruo Kiyoi
1   Department of Internal Medicine and Molecular Science, Graduate School of Medicine, Osaka University, Osaka, and Department of Blood Transfusion, Osaka University Hospital, Osaka, Japan
,
Yoshiyuki Kurata
1   Department of Internal Medicine and Molecular Science, Graduate School of Medicine, Osaka University, Osaka, and Department of Blood Transfusion, Osaka University Hospital, Osaka, Japan
,
Yuji Matsuzawa
1   Department of Internal Medicine and Molecular Science, Graduate School of Medicine, Osaka University, Osaka, and Department of Blood Transfusion, Osaka University Hospital, Osaka, Japan
› Author Affiliations
Further Information

Publication History

Received 15 March 2000

Accepted after resubmission 03 August 2000

Publication Date:
08 December 2017 (online)

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Summary

In chronic immune thrombocytopenic purpura (ITP), anti-GPIIb-IIIa (αIIbβ3) autoantibodies have been detected in serum and/or platelet-associated IgG (PAIgG) and considered as one of the major causes. We examined whether anti-αvβ3 antibodies might be present in ITP cases because of the similarity between αvβ3 and GPIIb-IIIa (αIIbβ3). Modified antigen capture ELISA (MACE) using human umbilical vein endothelial cells (HUVEC) showed the presence of serum anti-αvβ3 antibodies in 23 of 80 ITP patients (29%). Cross-adsorption studies between platelets and HUVEC demonstrated that most of anti-αvβ3 and anti-GPIIb-IIIa antibodies exclusively reacted with αvβ3 and GPIIb-IIIa, respectively. Platelet-associated anti-GPIIb-IIIa antibodies did not react with αvβ3, either. Interestingly, patients having anti-αvβ3 antibodies showed significantly lower platelet counts than negative patients. These results indicate the serum anti-αvβ3 antibodies are different ones from the classical anti-GPIIb-IIIa (αIIbβ3) antibodies and would provide a new insight into the pathophysiology of ITP as well as the autoantigenic epitopes on β3 integrins.

Keywords

ITP - autoantibody - β3 integrin - GPIIb-IIIa - αvβ3
 

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