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Thromb Haemost 1999; 81(06): 984-988
DOI: 10.1055/s-0037-1614610
DOI: 10.1055/s-0037-1614610
Letters to the Editor
Stimulation of Proteinase Activated Receptor-2 Causes Endothelial Cells to Promote Blood Coagulation In Vitro
Further Information
Publication History
Received
07 December 1998
Accepted after revision
23 February 1999
Publication Date:
09 December 2017 (online)
Summary
Proteolytically activated receptors define a new subclass among the G-protein coupled receptors. Proteinase activated receptor-2 (PAR-2), the second member to be identified of this growing receptor subclass, can be activated by trypsin and trypsin-like serine proteases such as mast cell tryptase. PAR-2 is expressed in endothelial cells. Here we have studied if activation of PAR-2 changes the coagulation properties of cultured human umbilical vein endothelial cells. We show that activation of PAR-2 induces rapid and transient formation of tissue factor mRNA with a maximum level 1 hour after receptor stimulation. The increased mRNA level was accompanied by an increased tissue factor activity at the endothelial cell surface, shortening coagulation time in a standard clotting assay. The level of tissue factor activity after PAR-2 activation was comparable with the effects of thrombin receptor (PAR-1) activation although neither of the two protease receptors were as strong inducers of tissue factor as tumor necrosis factor-α.
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References
- 1 Edgington TS, Mackman N, Brand K, Ruf W. The structural biology of expression and function of tissue factor. Thromb Haemost 1991; 66: 67-79.
- 2 Colucci M, Balconi G, Lorenzet R, Pietra A, Locati D, Donati MB, Semeraro N. Cultured human endothelial cells generate tissue factor in response to endotoxin. J Clin Invest 1983; 71: 1893-6.
- 3 Vu T-KH, Hung DT, Wheaton I V, Coughlin SR. Molecular cloning of a functional thrombin receptor reveals a novel proteolytic mechanism of receptor activation. Cell 1991; 64: 1057-68.
- 4 Nystedt S, Emilsson K, Wahlestedt C, Sundelin J. Molecular cloning of a potential proteinase activated receptor. Proc Natl Acad Sci USA 1994; 91: 9208-12.
- 5 Ishihara H, Connolly AJ, Zeng D, Kahn ML, Zheng YW, Timmons C, Tram T, Coughlin SR. Protease-activated receptor 3 is a second thrombin receptor in humans. Nature 1997; 386: 502-6.
- 6 Xu W-F. Andersen H, Whitmore TE, Presnell SR, Yee DP, Ching A, Gilbert T, Davie EW, Foster DC. Cloning and characterization of human protease-activated receptor 4. Proc Natl Acad Sci USA 1998; 95: 6642-6.
- 7 Molino M, Barnathan ES, Numerof R, Clark J, Dreyer M, Cumashi A, Hoxie JA, Schechter N, Woolkalis M, Brass LF. Interactions of mast cell tryptase with thrombin receptors and PAR-2. J Biol Chem 1997; 272: 4043-9.
- 8 D’Andrea MR, Derian CK, Leturcq D, Baker SM, Brunmark A, Ling P, Darrow AL, Santulli RJ, Brass LF, Andrade-Gordon P. Characterization of protease-activated receptor-2 inmmunoreactivity in normal human tissues. J Histochem Cytochem 1998; 46: 157-64.
- 9 Molino M, Woolkalis MJ, Reavey-Cantwell J, Praticó D, Andrade-Gordon P, Barnathan ES, Brass LF. Endothelial cell thrombin receptors and PAR-2; two protease-activated receptors located in a single cellular environment. J Biol Chem 1997; 272: 11133-41.
- 10 Nystedt S, Ramakrishnan V, Sundelin J. The proteinase-activated receptor 2 is induced by inflammatory mediators in human endothelial cells. J Biol Chem 1996; 271: 14910-5.
- 11 Scarborough RM, Naughton MA, Teng W, Hung DT, Rose J, Vu T-KH, Wheaton I V, Turck CW, Coughlin SR. Thethered ligand agonist peptides. Structural requirements for thrombin receptor activation reveal mechanism of proteolytic unmasking of agonist function. J Biol Chem 1992; 267: 13146-9.
- 12 Jaffe EA, Nachman RL, Becker CG, Minick RC. Culture of human endothelial cells derived from umbilical veins. J Clin Invest 1973; 52: 2745-56.
- 13 Thornton SC, Mueller SN, Levine EM. Human endothelial cells: use of heparin in cloning and long-term serial cultivation. Science 1983; 222: 623-5.
- 14 Xie WQ, Rothblum I L. Rapid, small-scale RNA isolation from tissue culture cells. BioTechniques 1991; 11: 325-7.
- 15 Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K.. Current protocols in molecular biology. John Wiley & Sons, Inc.; 1994
- 16 Dejana E, Bussolino F, Wang JM, Mantovani A. Cytokine regulation of endothelial cells. In Cytokines a practical approach Balkwill FR. ed IRL Press at Oxford University Press; 1991: 215-28.
- 17 Nawroth PP, Handley DA, Esmon CT, Stern DM. Interleukin 1 induces endothelial cell procoagulant while suppressing cell-surface anticoagulant activity. Proc Natl Acad Sci USA 1986; 83: 3460-4.
- 18 Archipoff G, Beretz A, Freyssinet J-M, Klein-Soyer C, Brisson C, Caze-nave J-P.. Heterogeneous regulation of constitutive thrombomodulin or inducible tissue-factor activities on the surface of human saphenous-vein endothelial cells in culture following stimulation by interleukin-1, tumor necrosis factor, thrombin or phorbol ester. Biochem J 1991; 273:: 679-84.
- 19 Blackhart BD, Emilsson K, Ngyuen D, Teng W, Martelli AJ, Nystedt S, Sundelin J, Scarborough RM. Ligand cross-reactivity within the protease-activated receptor family. J Biol Chem 1996; 271: 16466-71.
- 20 Østerud B, Flaegstad T. Increased tissue thromboplastin activity in mono-cytes of patients with meningococcal infection: Related to an unfavorable prognosis. Thromb Haemost 1983; 49: 5-7.
- 21 Drake TA, Cheng J, Chang A, Taylor Jr FB. Expression of tissue factor, thrombomodulin and E-selectin in baboons with lethal Escherichia coli sepsis. Am J Path 1993; 142: 1458-70.
- 22 Taylor Jr FB, Chang A, Ruf W, Morrissey JH, Hinshaw L, Catlett R, Blick K, Edgington TS. Lethal E. Coli septic shock is prevented by blocking tissue factor with monoclonal antibody. Circ Shock 1991; 33: 127-34.
- 23 Contrino J, Hair G, Kreutzer DL, Rickles FR. In situ detection of tissue factor in vascular endothelial cells: Correlation with the malignant phenotype of human breast disease. Nat Med 1996; 2: 209-14.
- 24 Solovey A, Gui L, Key NS, Hebbel RP. Tissue factor expression by endothelial cells in sickle cell anemia. J Clin Invest 1998; 101: 1899-904.
- 25 Hatakeyama K, Asada Y, Marutsuka K, Sato Y, Kamikubo Y, Sumiyoshi A. Localization and activity of tissue factor in human aortic atherosclerotic lesions. Atherosclerosis 1997; 133: 213-9.
- 26 Bugge TH, Xiao Q, Kombrinck KW, Flick MJ, Holmbäck K, Danton MJS, Colbert MC, Witte DP, Fujikawa K, Davie EW, Degen JL. Fatal embryonic bleeding events in mice lacking tissue factor, the cell-associated initiator of blood coagulation. Proc Natl Acad Sci USA 1996; 93: 6258-63.
- 27 Carmeliet P, Mackman N, Moons L, Luther T, Gressens P, Van Vlaenderen I, Demunck H, Kasper M, Breier G, Evrard P, Müller M, Risau W, Edgington T, Collen D. Role of tissue factor in embryonic blood vessel development. Nature 1996; 383: 73-5.
- 28 Suh TT, Holmbäck K, Jensen NJ, Daugherty CC, Small K, Simon DI, Potter SS, Degen JL. Resolution of spontaneous bleeding events but failure of pregnancy in fibrinogen-deficient mice. Gen Dev 1995; 9: 2020-33.
- 29 Rosen ED, Chan JCY, Idusogie E, Clotman F, Vlasuk G, Luther T, Jalbert LR, Albrecht S, Zhong L, Lissens A, Schoonjans L, Moons L, Collen D, Castellino FJ, Carmeliet P. Mice lacking factor VII develop normally but suffer fatal perinatal bleeding. Nature 1997; 390: 290-3.
- 30 Emilsson K, Wahlestedt C, Sun M, Nystedt S, Owman C, Sundelin J. Vascular effects of proteinase-activated receptor 2 agonist peptide. J Vasc Res 1997; 34: 267-72.
- 31 Muramatsu I, Laniyonu A, Moore GJ, Hollenberg MD. Vascular actions of thrombin receptor peptide. Can J Physiol Pharmacol 1992; 70: 996-1003.