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Thromb Haemost 2018; 118(03): 480-489
DOI: 10.1055/s-0038-1629901
Coagulation and Fibrinolysis
Schattauer GmbH Stuttgart

Effects of Low- and High-Dose Chemotherapy Agents on Thrombogenic Properties of Extracellular Vesicles Derived from Breast Cancer Cell Lines

Anat Aharon
,
Anni Rebibo Sabbah
,
Liron Issman
,
Hila Berkovich
,
Reem Copty
,
Yeshayahu Talmon
,
Benjamin Brenner
 Grants or Other Financial Support This study was supported by the Israel Science Foundation (ISF), grant no. 1413/21, and Rappaport Family Institute Grant 2012–2014.
Further Information

Publication History

11 September 2017

02 January 2018

Publication Date:
28 February 2018 (online)

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Abstract

Background The involvement of extracellular vesicles (EVs) in cancer-associated thrombosis (CT) is unclear. This study aimed to explore the properties of EVs derived from breast cancer (BC) cells following exposure to high- or low-dose chemotherapeutic agents and evaluate thrombogenic effects of these EVs on endothelial cells (ECs).

Methods EVs were isolated from BC cell lines (non-metastatic MCF7, high-metastatic MDA-MB-231), pre-exposed to serum-free medium (control), with or without increasing doses of doxorubicin or paclitaxel. EV structure and size were studied using electron microscopy and Nano-sight. Antigen levels were measured by fluorescence-activated cell sorting (FACS). EV effects on EC thrombogenicity were assessed using FACS, factor Xa chromogenic assay and RT-PCR.

Results Serum-free medium BC cell resulted in EV shedding that additionally increased when MDA-MB-231 cells were exposed to high doses of both agents. Tissue factor (TF) levels were similarly low (9–13%) in all EVs compared with the high expression on their parental MDA-MB-231 cells (76–83%). EVs derived from MDA-MB-231 cells stimulated with high-dose doxorubicin demonstrated significantly (fivefold; p < 0.001) elevated levels of negatively charged phospholipids, a 97% decrease in TF pathway inhibitor (TFPI) levels and a sixfold increase (p < 0.001) in procoagulant activity. These EVs also enhanced EC thrombogenicity. Effects of EVs originating from MCF7 cells were less pronounced.

Conclusion These findings suggest that thrombogenic properties of BC-derived EVs may depend on the type and dose of the applied chemotherapy agent and may also be affected by the cell metastatic nature.

Keywords

extracellular vesicles - breast cancer cells - endothelial cells - high-dose chemotherapy - thrombogenicity
 

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