Subscribe to RSS
Download PDF
DOI: 10.1055/s-0038-1632539
Comparison of Histomorphometric Measurements of Equine Sternal Cancellous Bone with Bone from other Skeletal Sites
Publication History
Received:03 October 1997
Accepted:05 January 1998
Publication Date:
10 February 2018 (online)
Summary
Histomorphometric analysis of bone biopsy specimens in humans is a reliable techniqu e which provides information on bone status and turnover. A techniqu e of sternal biopsy has been recently developed in “standin g horses” . The purpose of the present study was to demonstrate that the histomorphometric analysis of sternal cancellous bone was representative of other skeletal sites in horses.
Post-mortem cancellous bone samples were obtained from the sternum, 12th rib, ilium, 1st lumbar vertebra and the tibia, of seven adult horses and embedded in methylmethacrylate. Sections, 7 μm thick, were cut and were stained with Goldner’s green trichrome. Histomorphometric parameters were measured by a semi-automated method. Sections from the sternum were qualitativel y similar to those from the rib, ilium and vertebra. Histomorphometric measurements were easily performed on the sternal, ilium, rib and vertebral sections. The tibial sections were not suitable for evaluation. The sternal histomorphometric parameters of bone formation and resorption were overall representative of those from other skeletal sites.
The present study demonstrated that sternal cancellous bone is acceptable for bone histomorphometry in horses as it reflects overall skeletal osseous activity.
This study compares qualitatively and quantitatively cancellous bone from the sternum, 12th rib, ilium, first lumbar vertebra and the tibia of seven adult horses. Sternal cancellous bone is acceptable for bone histomorphometry and reflects overall skeletal osseous activity.
-
REFERENCES
- 1 Anderson C, Danylchuk KD. Bone-remodeling rates of the Beagle: a comparison between different sites on the same rib. Am J Vet Res 1978; 39: 1763-5.
- 2 Carter DH, Barnes JM, Aaron JE. Histomorphometry of fresh frozen iliac crest bone biopsies. Calcif Tissue Int 1989; 44: 387-92.
- 3 Chavassieux PM, Arlot ME, Meunier PJ. Intersample variation in bone histomorphometry: comparison between parameter values measured on two contiguous transiliac bone biopsies. Calcif Tissue Int 1985; 37: 345-50.
- 4 Ching SV, Norrdin RW. Histomorphometric comparison of measurements of trabecular bone remodeling in iliac crest biopsy sites and lumbar vertebrae in cats. Am J Vet Res 1990; 51: 447-50.
- 5 Christiansen P, Steiniche T, Vesterby A, Mosekilde L, Hessov I, Melsen F. Primary hyperparathyroidism: iliac crest trabecular bone volume, structure, remodeling, and balance evaluated by histomorphometric methods. Bone 1992; 13: 41-9.
- 6 Compston JE, Vedi S, Stellon AJ. Interobserver and intra-observer variation in bone histomorphometry. Calcif Tissue Int 1986; 38: 67-70.
- 7 Désévaux C. Étude du sternum comme site de biopsie osseuse chez le cheval debout et représentativé de l'os trabéculaire sternal à l'analyse histomorphométrique. Master's degree, University of Montreal 1996
- 8 Kimmel DB, Recker RR, Lappe JM. Histomorphometry of normal pre- and postmenopausal women. Bone 1992; 13 A 18.
- 9 Malluche HH, Faugere MC. Methodology of mineralized bone histology. In Atlas of Mineralized Bone Histology Malluche HH, Faugère MC. (eds). Basel: Karger; 1986: 17-48.
- 10 Malluche HH, Meyer W, Sherman D, Massry SG. Quantitative bone histology in 84 normal American subjects. Micromorphometric analysis and evaluation of variance in iliac bone. Calcif Tissue Int 1982; 34: 449-55.
- 11 Malluche HH, Sherman D, Meyer W, Massry SG. A new semiautomatic method for quantitative static and dynamic bone histology. Calcif Tissue Int 1982; 34: 439-48.
- 12 Meghji S. Bone remodelling. Br Dent J 1992; 172: 235-42.
- 13 Mellish RWE, Ferguson-Pell MW, Cochran GVB, Lindsay R, Dempster DW. A new manual method for assessing two-dimensional cancellous bone structure: comparison between iliac crest and lumbar vertebra. J Bone Miner Res 1991; 6: 689-96.
- 14 Melsen F, Mosekilde L. The role of bone biopsy in the diagnosis of metabolic bone disease. Orthop Clin North Am 1981; 12: 571-602.
- 15 Misheff MM, Stover SM, Pool RR. Corticocancellous bone biopsy from the 12th rib of standing horses. Vet Surg 1992; 21: 133-8.
- 16 Moore RJ, Durbridge TC, Woods AE, Vernon-Roberts B. Variation in histomorphometric estimates across different sites of the iliac crest. J Clin Pathol 1989; 42: 814-6.
- 17 Parfitt AM, Drezner MK, Glorieux FH, Kanis JA, Malluche H, Meunier PJ, Ott SM, Recker RR. Bone histomorphometry: standardization of nomenclature, symbols, and units. Report of the ASBMR Histomorphometry Nomenclature Committee. J Bone Miner Res 1987; 2: 595-610.
- 18 Parisien MV, McMahon D, Pushparaj N, Dempster DW. Trabecular architecture in iliac crest bone biopsies: infra-individual variability in structural parameters and changes with age. Bone 1988; 9: 289-95.
- 19 Pødenphant J, Engel U. Regional variations in histomorphometric bone dynamics from the skeleton of an osteoporotic woman. Calcif Tissue Int 1987; 40: 184-8.
- 20 Pødenphant J, Gotfredsen A, Nilas L, Nrgaard H, Braendstrup O. Iliac crest biopsy: representativity for the amount of mineralized bone. Bone 1986; 7: 427-30.
- 21 Pødenphant J, Gotfredsen A, Nilas L, Nrgrd H, Braendstrup O, Christiansen C. Iliac crest biopsy: an investigation on certain aspects of precision and accuracy. Bone Miner 1986; 1: 279-87.
- 22 Recker RR. Bone biopsy and histomorphometry in clinical practice. Rheum Dis Clin North Am 1994; 20: 609-27.
- 23 Recker RR, Kimmel DB, Parfitt AM, Davies KM, Keshawarz N, Hinders S. Static and tetracycline-based bone histomorphometric data from 34 normal postmenopausal females. J Bone Miner Res 1988; (03) 133-44.
- 24 Richardson GL, Pool RR, Pascoe JR. Wheat JD. Autogenous cancellous bone grafts from the sternum in horses. Vet Surg 1986; 15: 9-15.
- 25 Savage CJ, Jeffcott LB, Melsen F, Østblom LC. Bone biopsy in the horse. 1. Method using the wing of ilium. J Vet Med 1991; A38: 776-83.
- 26 Savage CJ, McCarthy RN, Jeffcott LB. Histomorphometric assessment of bone biopsies from foals fed diets high in phosphorus and digestible energy. Equine Vet J 1993; (Suppl. 16) Suppl 89-93.
- 27 Savage CJ, Tidd LC, Melsen F, Jeffcott LB, Østblom L. Bone biopsy in the horse. 2. Evaluation of histomorphometric examination. J Vet Med 1991; A38: 784-92.
- 28 Savage CJ, Tidd LC, Østblom LC, Melsen F, Jeffcott LB. Bone biopsy in the horse. 3. Normal histomorphometric data according to age and sex. J Vet Med 1991; A38: 793-7.
- 29 Sedlin ED, Frost HM, Villanueva AR. The eleventh rib biopsy in the study of metabolic bone disease. Henry Ford Hosp Med Bull 1963; 11: 217-9.
- 30 Vesterby A. Star volume of marrow space and trabeculae in iliac crest: sampling procedure and correlation to star volume of first lumbar vertebra. Bone 1990; 11: 149-55.
- 31 Vesterby A, Gundersen HJ, Melsen F. Star volume of marrow space and trabeculae of the first lumbar vertebra: sampling efficiency and biological variation. Bone 1989; 10: 7-13.
- 32 Vesterby A, Gundersen HJ, Melsen F, Mosekilde L. Normal post-menopausal women show iliac crest trabecular thickening on vertical sections. Bone 1989; 10: 333-9.
- 33 Weinstein RS. Clinical use of bone biopsy. In Disorders of bone and mineral metabolism Coe FL, Favus MJ. (eds). New York: Raven Press; 1992: 455-74.
- 34 Wright CD, Vedi S, Garrahan NJ, Stanton M, Duffy SW, Compston JE. Combined inter-observer and inter-method variation in bone histomorphometry. Bone 1992; 13: 205-8.